Details of the Target

General Information of Target

Target ID LDTP10870
Target Name Neurogenic locus notch homolog protein 4 (NOTCH4)
Gene Name NOTCH4
Gene ID 4855
Synonyms
INT3; Neurogenic locus notch homolog protein 4; Notch 4; hNotch4) [Cleaved into: Notch 4 extracellular truncation; Notch 4 intracellular domain]
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MITSAAGIISLLDEDEPQLKEFALHKLNAVVNDFWAEISESVDKIEVLYEDEGFRSRQFA
ALVASKVFYHLGAFEESLNYALGAGDLFNVNDNSEYVETIIAKCIDHYTKQCVENADLPE
GEKKPIDQRLEGIVNKMFQRCLDDHKYKQAIGIALETRRLDVFEKTILESNDVPGMLAYS
LKLCMSLMQNKQFRNKVLRVLVKIYMNLEKPDFINVCQCLIFLDDPQAVSDILEKLVKED
NLLMAYQICFDLYESASQQFLSSVIQNLRTVGTPIASVPGSTNTGTVPGSEKDSDSMETE
EKTSSAFVGKTPEASPEPKDQTLKMIKILSGEMAIELHLQFLIRNNNTDLMILKNTKDAV
RNSVCHTATVIANSFMHCGTTSDQFLRDNLEWLARATNWAKFTATASLGVIHKGHEKEAL
QLMATYLPKDTSPGSAYQEGGGLYALGLIHANHGGDIIDYLLNQLKNASNDIVRHGGSLG
LGLAAMGTARQDVYDLLKTNLYQDDAVTGEAAGLALGLVMLGSKNAQAIEDMVGYAQETQ
HEKILRGLAVGIALVMYGRMEEADALIESLCRDKDPILRRSGMYTVAMAYCGSGNNKAIR
RLLHVAVSDVNDDVRRAAVESLGFILFRTPEQCPSVVSLLSESYNPHVRYGAAMALGICC
AGTGNKEAINLLEPMTNDPVNYVRQGALIASALIMIQQTEITCPKVNQFRQLYSKVINDK
HDDVMAKFGAILAQGILDAGGHNVTISLQSRTGHTHMPSVVGVLVFTQFWFWFPLSHFLS
LAYTPTCVIGLNKDLKMPKVQYKSNCKPSTFAYPAPLEVPKEKEKEKVSTAVLSITAKAK
KKEKEKEKKEEEKMEVDEAEKKEEKEKKKEPEPNFQLLDNPARVMPAQLKVLTMPETCRY
QPFKPLSIGGIIILKDTSEDIEELVEPVAAHGPKIEEEEQEPEPPEPFEYIDD
Target Type
Clinical trial
Target Bioclass
Other
Family
NOTCH family
Subcellular location
Nucleus; Cell membrane
Function
Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system.
TTD ID
T49453
Uniprot ID
Q99466
DrugMap ID
TTXDIK2
Ensemble ID
ENST00000375023.3
HGNC ID
HGNC:7884
ChEMBL ID
CHEMBL3407321

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
22RV1 SNV: p.G1906Ter .
A2780 SNV: p.R1665Q .
AN3CA SNV: p.D769G .
CCK81 Deletion: p.G1891AfsTer13 .
CHL1 SNV: p.P995S .
COLO678 Deletion: p.E662LfsTer14
SNV: p.S913P		 .
COLO679 SNV: p.S676L .
HDMYZ SNV: p.A1799V .
HT115 SNV: p.S743F .
ICC3 SNV: p.Q1768L .
JURKAT Deletion: p.L628CfsTer65; p.P1485LfsTer20 .
LNCaP clone FGC SNV: p.P762L .
MDST8 SNV: p.P240S .
MELHO Deletion: p.K207PfsTer43 .
MEWO Insertion: p.C133LfsTer20
SNV: p.S1118R		 .
MFE296 Deletion: p.K2003NfsTer13 .
NALM6 SNV: p.P1397S .
NCIH1155 SNV: p.A162T .
NCIH2286 SNV: p.L389M .
NCIH23 SNV: p.P1049T .
NOMO1 SNV: p.I1509L .
OVK18 Deletion: p.G1154AfsTer150 .
RL952 SNV: p.P1994S .
SHP77 Substitution: p.G426A .
SKMEL2 SNV: p.R1158Ter .
SKMM2 SNV: p.P124S .
SKNSH SNV: p.L1152F .
SW948 SNV: p.M1338I .
TE4 SNV: p.G1603E; p.G1607A .
TYKNU SNV: p.A1347T .
UMUC3 SNV: p.A1650G .
YSCCC SNV: p.G1352A .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
OPA-S-S-alkyne
 Probe Info  		K1372(9.22)  LDD3494  [1]

References

1 A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell. Nat Commun. 2024 Apr 8;15(1):2997. doi: 10.1038/s41467-024-47033-w.
Mass spectrometry data entry: PXD042888