Details of the Target

General Information of Target

Target ID LDTP10742
Target Name Alpha-protein kinase 1 (ALPK1)
Gene Name ALPK1
Gene ID 80216
Synonyms
KIAA1527; LAK; Alpha-protein kinase 1; EC 2.7.11.1; Chromosome 4 kinase; Lymphocyte alpha-protein kinase
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MPTTQQSPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSP
KSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVK
SFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTRNILPDEGEPTDEETTGDISDSM
DFVLLNFAEMNKLWVRMQHQGHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQ
IVLTGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIII
ALIDRLALFAHREDGPGIPADIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMK
CYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKH
FHPLFEYFDYESRKSMSCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPDQPVEDPD
PEDFADEQSLVGRFIHLLRSEDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLA
FRYKENSKVDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIGFENHE
TVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRTQCALAASKL
LKKPDQGRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECLKKALKIANQCMDPSLQVQLF
IEILNRYIYFYEKENDAVTIQVLNQLIQKIREDLPNLESSEETEQINKHFHNTLEHLRLR
RESPESEGPIYEGLIL
Target Bioclass
Enzyme
Family
Protein kinase superfamily, Alpha-type protein kinase family, ALPK subfamily
Subcellular location
Cytoplasm, cytosol
Function
Serine/threonine-protein kinase that detects bacterial pathogen-associated molecular pattern metabolites (PAMPs) and initiates an innate immune response, a critical step for pathogen elimination and engagement of adaptive immunity. Specifically recognizes and binds ADP-D-glycero-beta-D-manno-heptose (ADP-Heptose), a potent PAMP present in all Gram-negative and some Gram-positive bacteria. ADP-Heptose-binding stimulates its kinase activity to phosphorylate and activate TIFA, triggering pro-inflammatory NF-kappa-B signaling. May be involved in monosodium urate monohydrate (MSU)-induced inflammation by mediating phosphorylation of unconventional myosin MYO9A. May also play a role in apical protein transport by mediating phosphorylation of unconventional myosin MYO1A. May play a role in ciliogenesis.
Uniprot ID
Q96QP1
Ensemble ID
ENST00000177648.13
HGNC ID
HGNC:20917

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
BXPC3 Substitution: p.R1084Q .
DU145 SNV: p.Q453E; p.A798S; p.S835N .
HCT116 Deletion: p.S938PfsTer14 .
HCT15 SNV: p.V7A; p.F61S; p.K1140Ter .
HT1197 SNV: p.V421I .
IPC298 SNV: p.R718C; p.P881S .
MCC13 SNV: p.G986R .
MDAMB453 Substitution: p.R1084Q .
MEWO SNV: p.E752K .
MFE319 Insertion: p.S1022IfsTer2 .
NB4 Substitution: p.R1084Q .
NCIH446 Substitution: p.R1084Q .
PC3 Substitution: p.R1084Q .
SNU308 SNV: p.G681V .
UACC257 Substitution: p.R1084Q .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C326(3.16)  LDD3397  [1]
IA-alkyne
 Probe Info  		C512(8.26)  LDD1706  [2]
IPM
 Probe Info  		N.A.  LDD2156  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0625  F8 Ramos C512(1.04)  LDD2187  [4]
 LDCM0574  Fragment12 Ramos C512(1.41)  LDD2191  [4]
 LDCM0575  Fragment13 Ramos C512(1.12)  LDD2192  [4]
 LDCM0576  Fragment14 Ramos C512(2.40)  LDD2193  [4]
 LDCM0580  Fragment21 Ramos C512(0.87)  LDD2195  [4]
 LDCM0582  Fragment23 Ramos C512(1.15)  LDD2196  [4]
 LDCM0578  Fragment27 Ramos C512(1.00)  LDD2197  [4]
 LDCM0586  Fragment28 Ramos C512(0.56)  LDD2198  [4]
 LDCM0588  Fragment30 Ramos C512(0.97)  LDD2199  [4]
 LDCM0589  Fragment31 Ramos C512(1.49)  LDD2200  [4]
 LDCM0468  Fragment33 Ramos C512(0.96)  LDD2202  [4]
 LDCM0614  Fragment56 Ramos C512(1.71)  LDD2205  [4]
 LDCM0022  KB02 AU565 C326(2.05)  LDD2265  [1]
 LDCM0023  KB03 AU565 C326(2.90)  LDD2682  [1]
 LDCM0024  KB05 PF-382 C326(3.16)  LDD3397  [1]

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
3 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
4 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578