Details of the Target

General Information of Target

Target ID LDTP10627
Target Name Protein broad-minded (TBC1D32)
Gene Name TBC1D32
Gene ID 221322
Synonyms
BROMI; C6orf170; C6orf171; Protein broad-minded; TBC1 domain family member 32
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAAVILPSTAAPSSLFPASQQKGHTQGGELVNELLTSWLRGLVTFEDVAVEFTQEEWALL
DPAQRTLYRDVMLENCRNLASLGCRVNKPSLISQLEQDKKVVTEERGILPSTCPDLETLL
KAKWLTPKKNVFRKEQSKGVKTERSHRGVKLNECNQCFKVFSTKSNLTQHKRIHTGEKPY
DCSQCGKSFSSRSYLTIHKRIHNGEKPYECNHCGKAFSDPSSLRLHLRIHTGEKPYECNQ
CFHVFRTSCNLKSHKRIHTGENHHECNQCGKAFSTRSSLTGHNSIHTGEKPYECHDCGKT
FRKSSYLTQHVRTHTGEKPYECNECGKSFSSSFSLTVHKRIHTGEKPYECSDCGKAFNNL
SAVKKHLRTHTGEKPYECNHCGKSFTSNSYLSVHKRIHNRWI
Target Bioclass
Other
Subcellular location
Cytoplasm
Function
Required for high-level Shh responses in the developing neural tube. Together with CDK20, controls the structure of the primary cilium by coordinating assembly of the ciliary membrane and axoneme, allowing GLI2 to be properly activated in response to Shh signaling.
Uniprot ID
Q96NH3
Ensemble ID
ENST00000275159.11
HGNC ID
HGNC:21485

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
AN3CA SNV: p.E992D; p.V1244A .
HCC15 SNV: p.T580S .
HCT15 SNV: p.D927N .
HEC1 SNV: p.S785T .
HEC1B SNV: p.S785T .
LNCaP clone FGC SNV: p.K1027Ter .
LOXIMVI SNV: p.P654S .
LS180 Deletion: p.K747SfsTer9 .
MDAMB453 SNV: p.A920S .
MOLT4 Insertion: p.L718IfsTer7 .
NCIH1155 Insertion: p.R293SfsTer5 .
NCIH1792 SNV: p.E34D .
SNU1 SNV: p.Y848Ter .
TE4 SNV: p.G1079Ter .
TOV21G SNV: p.E7Ter .
UMUC3 SNV: p.R18L .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K717(5.00)  LDD0277  [1]
Acrolein
 Probe Info  		N.A.  LDD0217  [2]
Crotonaldehyde
 Probe Info  		N.A.  LDD0219  [2]
Methacrolein
 Probe Info  		N.A.  LDD0218  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [2]

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.