Details of the Target

General Information of Target

Target ID LDTP10598
Target Name Centrosomal protein of 63 kDa (CEP63)
Gene Name CEP63
Gene ID 80254
Synonyms
Centrosomal protein of 63 kDa; Cep63
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MKEPDDQDTDGEKSVTSKSDGKKSLRSSKSESRSPVQEDNTFLEDDTDETFTKGEGSYLE
EDSDEERLEGSLSSFQYGDLQSTTVPQQTPAPAVEEAEEEVKKKISESFFYDYMELASMP
FVTLDSNIPLDLLTLVHSFGYDCRKRANLQLLDDSIAIYIAGNQLIFLNLKTKEQIYLRS
SSGEGIGVIGVHPHKTYFTVAEKGSFPDIIIYEYPSLRPYRVLRDGTEKGYAYVDFNYSG
NLLASVGSNPDYTLTIWNWKEEQPILRTKAFSQEVFKVTFNPDKEEQLTTSGSGHIKFWE
MAFTFTGLKLQGSLGRFGKTITTDIEGYMELPDGKVLSGSEWGNMLLWEGGLIKVELCRG
TSKSCHNGPINQIMLYEGEVITVGSDGYVRIWDFETIDTADVIDETGLLEIEPINELQVD
KNVNLFSMIKMNETGNNFWLAQDANGAIWKLDLSFSNITQDPECLFSFHSGAIEAVAVSP
LTYLMATTALDCSVRIYDFASKTPLAQMKFKQGGTALVWVPRMVNFTGAQIIVGFEDGVV
RILELYDPKGLTIFAGRKKILDADIQLKQVFKPHTACVTALAYERDGEILATGSKDQTVF
FFEVERDYKPIGYINTPGPVCQLMWSPMSHPESTLLIICENGYILEAPLPTIKQEEDDHD
VVSYEIKDMCIKCFHFSSVKSKILRLIEIEKRERQRELKEKIREERRNKLAAEMGEDGEK
EFQEEEEEKEEEEEEEEPLPEIFIPSTPSPILCGFYSEPGKFWVSLGGYDSGFLYHCEFP
PCDESSDFKEQKDEPIDVRYLADTEDNPIQTITFNINKVMMFCGMKNGAIRVYVLNQNDP
SLTSLVDYWHFNMHDNNYGCIKSIANSFDDRFLVTAGADGNIFVFNIFSEFMLRKDMKAK
VPSPRFGIETEPIPEDIEDPKAYSIENARRKREHDKLMKEVGEIKARKREQIKALRSEFC
NLLEMNEKLPKHMQFKRTDFDVDSQIRAEMHRKTAFKIQQVEKELAWEKEKHELGLMKLK
NRFRDPLESDTIVVHAILSDHKISSYRLVQPSKYSKFKRASQSERKPSKLDRFEKEGPGR
KDSQRDAGGSVTIQEESIIEKGKKFRPKTLSEIIVENQIEKTRKLILKAERAQLKIQQRK
KEWEELYKSKPGDDYEDPKDLQAIKEAQVYMGDFNLKTAPDYKIPEHMRINAAKKEEELG
HLDSLVHGNKRHMNKCILSLRDLKVAVVEEIQCLVQELKNIQSTLHISKHIPIPKIPQIH
PEEVPEKRFQYDEETLLNFKQQQMKSKDEKSPGVEQTGSGGPVGGFLKLSSRKDGDLTTR
DSISRSSKASTFSLDIPKCLEFEKAEPTDVELEIMKRDEIKHVYMQQYLVNRIKELVVTF
DAELRLLRHQKLKLDTQMKLSDLHHVTLFQEILLLKNFEKQENILQERVNSLDKEEQYMQ
WKINETLKEMEEKKNEITKLQEQEKALYAGFQAAIGENNKFANFLMKVLKKKIKRVKKKE
VEGDADEDEESEESSEEESSLESDEDESESEDEVFDDSICPTNCDVALFELALHLREKRL
DIEEALVEEKKIVDNLKKEYDTLSKKVKIVATNLNAAEEALEAYQREKQQRLNELLVVIP
LKLHQIEYVVFGEIPSDLSGTLVFSNHALRRLQERIHELQEENSKQQKLNKEWRERRKQL
IREKREMTKTIHKMEETVRQLMISKFGRVVNLEALQTLSVNTTLEELKIRKLRKELANAK
EMKMWEEKIAQMRWELMMKTKEHTRKLYQMNDLCIEKKKLDSRLNTLQNQQGNAFQGPRE
ADVVAREEVTELIQLQAERISALKEEIALLRRKGSLILPPIQSPREKEIQPADL
Target Bioclass
Other
Family
CEP63 family
Subcellular location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Function
Required for normal spindle assembly. Plays a key role in mother-centriole-dependent centriole duplication; the function seems also to involve CEP152, CDK5RAP2 and WDR62 through a stepwise assembled complex at the centrosome that recruits CDK2 required for centriole duplication. Reported to be required for centrosomal recruitment of CEP152; however, this function has been questioned. Also recruits CDK1 to centrosomes. Plays a role in DNA damage response. Following DNA damage, such as double-strand breaks (DSBs), is removed from centrosomes; this leads to the inactivation of spindle assembly and delay in mitotic progression. Promotes stabilization of FXR1 protein by inhibiting FXR1 ubiquitination.
Uniprot ID
Q96MT8
Ensemble ID
ENST00000332047.10
HGNC ID
HGNC:25815

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
KYSE30 SNV: p.P559R .
OVK18 SNV: p.G355D .
REH SNV: p.R11Q .
RH30 SNV: p.G45A .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C108(4.29)  LDD0209  [1]
IPM
 Probe Info  		N.A.  LDD2156  [2]
TFBX
 Probe Info  		N.A.  LDD0148  [3]
YN-1
 Probe Info  		E668(0.00); E671(0.00)  LDD0447  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0022  KB02 HEK-293T C53(0.99); C108(0.97)  LDD1492  [5]
 LDCM0023  KB03 Jurkat C108(4.29)  LDD0209  [1]
 LDCM0024  KB05 OPM-2 C108(3.36)  LDD3377  [6]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 8 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Histone-lysine N-methyltransferase EZH2 (EZH2) Histone-lysine methyltransferase family Q15910
Dystrobrevin beta (DTNB) Dystrophin family O60941
DNA-directed RNA polymerase III subunit RPC3 (POLR3C) Eukaryotic RPC3/POLR3C RNA polymerase subunit family Q9BUI4
Polyunsaturated fatty acid 5-lipoxygenase (ALOX5) Lipoxygenase family P09917
Ubiquitin carboxyl-terminal hydrolase 2 (USP2) Peptidase C19 family O75604
Calpain-7 (CAPN7) Peptidase C2 family Q9Y6W3
Tumor susceptibility gene 101 protein (TSG101) Ubiquitin-conjugating enzyme family Q99816
E3 ubiquitin-protein ligase DZIP3 (DZIP3) . Q86Y13
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Huntingtin (HTT) Huntingtin family P42858
Transcription factor
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Zinc finger protein 774 (ZNF774) Krueppel C2H2-type zinc-finger protein family Q6NX45
Protein rogdi homolog (ROGDI) Rogdi family Q9GZN7
Teashirt homolog 3 (TSHZ3) Teashirt C2H2-type zinc-finger protein family Q63HK5
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1 (SMARCE1) . Q969G3
Other
Click To Hide/Show 27 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
CWF19-like protein 2 (CWF19L2) CWF19 family Q2TBE0
Death domain-associated protein 6 (DAXX) DAXX family Q9UER7
Protein FAM161A (FAM161A) FAM161 family Q3B820
Protein fem-1 homolog C (FEM1C) Fem-1 family Q96JP0
HAUS augmin-like complex subunit 1 (HAUS1) HAUS1 family Q96CS2
Phakinin (BFSP2) Intermediate filament family Q13515
Harmonin-binding protein USHBP1 (USHBP1) MCC family Q8N6Y0
Meiosis-specific nuclear structural protein 1 (MNS1) MNS1 family Q8NEH6
Kinetochore protein NDC80 homolog (NDC80) NDC80/HEC1 family O14777
Protein SSX3 (SSX3) SSX family Q99909
Synaptotagmin-17 (SYT17) Synaptotagmin family Q9BSW7
Alpha-taxilin (TXLNA) Taxilin family P40222
Beta-taxilin (TXLNB) Taxilin family Q8N3L3
Centrosomal protein CEP57L1 (CEP57L1) Translokin family Q8IYX8
Centrosomal protein of 57 kDa (CEP57) Translokin family Q86XR8
Arfaptin-2 (ARFIP2) . P53365
Coiled-coil domain-containing protein 14 (CCDC14) . Q49A88
Disrupted in schizophrenia 1 protein (DISC1) . Q9NRI5
Hepatocyte growth factor-regulated tyrosine kinase substrate (HGS) . O14964
KN motif and ankyrin repeat domain-containing protein 2 (KANK2) . Q63ZY3
NTF2-related export protein 2 (NXT2) . Q9NPJ8
Progesterone-induced-blocking factor 1 (PIBF1) . Q8WXW3
TBC1 domain family member 15 (TBC1D15) . Q8TC07
TBC1 domain family member 22B (TBC1D22B) . Q9NU19
Testis-specific protein 10-interacting protein (TSGA10IP) . Q3SY00
TRAF family member-associated NF-kappa-B activator (TANK) . Q92844
Zinc finger CCHC domain-containing protein 10 (ZCCHC10) . Q8TBK6

References

1 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
2 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
3 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
4 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
5 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
6 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840