Details of the Target

General Information of Target

Target ID LDTP10595
Target Name Roundabout homolog 3 (ROBO3)
Gene Name ROBO3
Gene ID 64221
Synonyms
Roundabout homolog 3; Roundabout-like protein 3
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MVVVTGREPDSRRQDGAMSSSDAEDDFLEPATPTATQAGHALPLLPQEFPEVVPLNIGGA
HFTTRLSTLRCYEDTMLAAMFSGRHYIPTDSEGRYFIDRDGTHFGDVLNFLRSGDLPPRE
RVRAVYKEAQYYAIGPLLEQLENMQPLKGEKVRQAFLGLMPYYKDHLERIVEIARLRAVQ
RKARFAKLKVCVFKEEMPITPYECPLLNSLRFERSESDGQLFEHHCEVDVSFGPWEAVAD
VYDLLHCLVTDLSAQGLTVDHQCIGVCDKHLVNHYYCKRPIYEFKITWW
Target Bioclass
Immunoglobulin
Family
Immunoglobulin superfamily, ROBO family
Subcellular location
Membrane
Function
Receptor involved in axon guidance during development. Acts as a multifunctional regulator of pathfinding that simultaneously mediates NELL2 repulsion, inhibits SLIT repulsion, and facilitates Netrin-1/NTN1 attraction. In spinal cord development plays a role in guiding commissural axons probably by preventing premature sensitivity to Slit proteins thus inhibiting Slit signaling through ROBO1/ROBO2. Binding OF NELL2 to the receptor ROBO3 promotes oligomerization of ROBO3, resulting in the repulsion of commissural axons in the midline. ROBO3 also indirectly boosts axon attraction to NTN1 without interacting with NTN1 itself.
Uniprot ID
Q96MS0
Ensemble ID
ENST00000397801.6
HGNC ID
HGNC:13433

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CAL51 SNV: p.A830T .
COLO320 Deletion: p.E244GfsTer6 .
HCC44 SNV: p.N477K .
HCT15 SNV: p.Y931H .
IPC298 SNV: p.G774R .
KYSE150 SNV: p.S974L .
MCC13 SNV: p.P933S; p.P973S .
MEWO SNV: p.G296R .
NCIH358 SNV: p.P764T .
REH SNV: p.R132C .
RKO SNV: p.R65C .
TOV21G SNV: p.P678S .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IPM
 Probe Info  		N.A.  LDD2156  [1]

References

1 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019