Details of the Target

General Information of Target

Target ID LDTP10592
Target Name NACHT, LRR and PYD domains-containing protein 4 (NLRP4)
Gene Name NLRP4
Gene ID 147945
Synonyms
NALP4; PAN2; PYPAF4; RNH2; NACHT, LRR and PYD domains-containing protein 4; Cancer/testis antigen 58; CT58; PAAD and NACHT-containing protein 2; PAN2; PYRIN and NACHT-containing protein 2; PYRIN-containing APAF1-like protein 4; PYPAF4; Ribonuclease inhibitor 2
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MALPACAVREFEPPRQPERGAPVRTTCPRRHSRVEAELAASRPGSVAASVRAGPPRGVSH
GFHTRPLLDKPRKASSSLAGAACAPLFALLSRGRRRRMHVLRRRWDLGSLCRALLTRGLA
ALGHSLKHVLGAIFSKIFGPMASVGNMDEKSNKLLLALVMLFLFAVIVLQYVCPGTECQL
LRLQAFSSPVPDPYRSEDESSARFVPRYNFTRGDLLRKVDFDIKGDDLIVFLHIQKTGGT
TFGRHLVRNIQLEQPCECRVGQKKCTCHRPGKRETWLFSRFSTGWSCGLHADWTELTSCV
PSVVDGKRDARLRPSRNFHYITILRDPVSRYLSEWRHVQRGATWKASLHVCDGRPPTSEE
LPSCYTGDDWSGCPLKEFMDCPYNLANNRQVRMLSDLTLVGCYNLSVMPEKQRNKVLLES
AKSNLKHMAFFGLTEFQRKTQYLFEKTFNMNFISPFTQYNTTRASSVEINEEIQKRIEGL
NFLDMELYSYAKDLFLQRYQFMRQKEHQEARRKRQEQRKFLKGRLLQTHFQSQGQGQSQN
PNQNQSQNPNPNANQNLTQNLMQNLTQSLSQKENRESPKQNSGKEQNDNTSNGTNDYIGS
VEKWR
Target Bioclass
Other
Family
NLRP family
Function
May be involved in inflammation and recognition of cytosolic pathogen-associated molecular patterns (PAMPs) not intercepted by membrane-bound receptors. Acts as a negative regulator of the type I interferon signaling pathway by serving as an adapter to promote DTX4-mediated ubiquitination of activated TBK1, and its subsequent degradation. Suppresses NF-kappaB induction by the cytokines TNFA and IL1B, suggesting that it operates at a point of convergence in these two cytokine signaling pathways.
Uniprot ID
Q96MN2
Ensemble ID
ENST00000301295.11
HGNC ID
HGNC:22943

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
COLO792 SNV: p.E875K .
DU145 SNV: p.R541L; p.L791I .
ECC2 SNV: p.S637R .
HEC1 SNV: p.V127I .
HEC1B SNV: p.V127I .
HS936T SNV: p.S678F .
HT3 SNV: p.K751E .
JHH7 SNV: p.I848V .
KPNYN SNV: p.L236M .
KYSE150 SNV: p.W44C; p.Q77L .
MCC13 SNV: p.G406D .
MELJUSO SNV: p.T72A; p.D562N .
MEWO SNV: p.P215T .
MOLM13 SNV: p.A914G .
NCIH1155 SNV: p.M322I .
NCIH2052 SNV: p.K959Q .
NCIH23 SNV: p.H472Y .
NCIH446 SNV: p.A816V .
RPMI7951 SNV: p.P978S .
TE4 SNV: p.P299L .
TOV21G SNV: p.D987G .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C479(3.72)  LDD3331  [1]
IA-alkyne
 Probe Info  		N.A.  LDD0150  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0022  KB02 AGS C99(1.56)  LDD2263  [1]
 LDCM0023  KB03 HGC-27 C99(2.05)  LDD2773  [1]
 LDCM0024  KB05 MKN-1 C479(3.72)  LDD3331  [1]

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Sequence-Based Prediction of Cysteine Reactivity Using Machine Learning. Biochemistry. 2018 Jan 30;57(4):451-460. doi: 10.1021/acs.biochem.7b00897. Epub 2017 Oct 26.