Details of the Target

General Information of Target

Target ID LDTP10559
Target Name Sphingomyelin synthase-related protein 1 (SAMD8)
Gene Name SAMD8
Gene ID 142891
Synonyms
Sphingomyelin synthase-related protein 1; SMSr; EC 2.7.8.-; Ceramide phosphoethanolamine synthase; CPE synthase; Sterile alpha motif domain-containing protein 8; SAM domain-containing protein 8
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSTEAQRVDDSPSTSGGSSDGDQRESVQQEPEREQVQPKKKEGKISSKTAAKLSTSAKRI
QKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSPDYPFKPPK
VTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQ
YMTNRAEHDRMARQWTKRYAT
Target Bioclass
Enzyme
Family
Sphingomyelin synthase family
Subcellular location
Endoplasmic reticulum membrane
Function
Sphingomyelin synthases synthesize sphingolipids through transfer of a phosphatidyl head group on to the primary hydroxyl of ceramide. SAMD8 is an endoplasmic reticulum (ER) transferase that has no sphingomyelin synthase activity but can convert phosphatidylethanolamine (PE) and ceramide to ceramide phosphoethanolamine (CPE) albeit with low product yield. Appears to operate as a ceramide sensor to control ceramide homeostasis in the endoplasmic reticulum rather than a converter of ceramides. Seems to be critical for the integrity of the early secretory pathway.
Uniprot ID
Q96LT4
Ensemble ID
ENST00000372687.4
HGNC ID
HGNC:26320

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
22RV1 SNV: p.P198S .
A3KAW SNV: p.M170V .
A673 SNV: p.V290G .
HCT15 SNV: p.I153M .
HEC1 SNV: p.C207Ter .
HEC1B SNV: p.C207Ter .
K562 SNV: p.N138K .
TE1 SNV: p.D125N .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IPM
 Probe Info  		N.A.  LDD0025  [1]
PPMS
 Probe Info  		N.A.  LDD0008  [2]
TFBX
 Probe Info  		N.A.  LDD0148  [1]
VSF
 Probe Info  		N.A.  LDD0007  [2]

References

1 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
2 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764