Details of the Target

General Information of Target

Target ID LDTP10558
Target Name Ubiquitin-conjugating enzyme E2 E2 (UBE2E2)
Gene Name UBE2E2
Gene ID 7325
Synonyms
UBCH8; Ubiquitin-conjugating enzyme E2 E2; EC 2.3.2.23; E2 ubiquitin-conjugating enzyme E2; UbcH8; Ubiquitin carrier protein E2; Ubiquitin-protein ligase E2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MEGTVESQTPDLRDVEGKVGRKTPEGLLRGLRGECELGTSGALLLPGASSTGHDLGDKIM
ALKMELAYLRAIDVKILQQLVTLNEGIEAVRWLLEERGTLTSHCSSLTSSQYSLTGGSPG
RSRRGSWDSLPDTSTTDRLDSVSIGSFLDTVAPSELDEQGPPGAPRSEMDWAKVIAGGER
ARTEVDVAATRLGSLRAVWKPPGERLQGGPPESPEDESAKLGFEAHWFWEQCQDDVTFL
Target Type
Literature-reported
Target Bioclass
Enzyme
Family
Ubiquitin-conjugating enzyme family
Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. Catalyzes the ISGylation of influenza A virus NS1 protein.
TTD ID
T90113
Uniprot ID
Q96LR5
DrugMap ID
TTXJEOF
Ensemble ID
ENST00000396703.6
HGNC ID
HGNC:12478

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 8 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		13.71  LDD0402  [1]
STPyne
 Probe Info  		K52(4.48)  LDD0277  [2]
IA-alkyne
 Probe Info  		N.A.  LDD0164  [3]
NAIA_5
 Probe Info  		C75(0.00); C81(0.00)  LDD2224  [4]
Acrolein
 Probe Info  		N.A.  LDD0217  [5]
Methacrolein
 Probe Info  		N.A.  LDD0218  [5]
W1
 Probe Info  		N.A.  LDD0236  [6]
AOyne
 Probe Info  		11.50  LDD0443  [7]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0156  Aniline NCI-H1299 14.51  LDD0403  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 12 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Ubiquitin thioesterase OTUB1 (OTUB1) Peptidase C65 family Q96FW1
E3 ubiquitin-protein ligase RNF5 (RNF5) RNF5 family Q99942
E3 ubiquitin-protein ligase Midline-1 (MID1) TRIM/RBCC family O15344
E3 ubiquitin-protein ligase TRIM39 (TRIM39) TRIM/RBCC family Q9HCM9
E3 ubiquitin-protein ligase TRIM50 (TRIM50) TRIM/RBCC family Q86XT4
Tripartite motif-containing protein 3 (TRIM3) TRIM/RBCC family O75382
E3 ubiquitin-protein ligase DZIP3 (DZIP3) . Q86Y13
E3 ubiquitin-protein ligase RING2 (RNF2) . Q99496
E3 ubiquitin-protein ligase RNF115 (RNF115) . Q9Y4L5
E3 ubiquitin-protein ligase RNF125 (RNF125) . Q96EQ8
E3 ubiquitin-protein ligase RNF185 (RNF185) . Q96GF1
NEDD4-like E3 ubiquitin-protein ligase WWP2 (WWP2) . O00308
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Arrestin domain-containing protein 3 (ARRDC3) Arrestin family Q96B67
Other
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein FAM9B (FAM9B) FAM9 family Q8IZU0
CUE domain-containing protein 1 (CUEDC1) . Q9NWM3
DAZ-associated protein 2 (DAZAP2) . Q15038

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
4 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
5 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
6 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
7 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.