Details of the Target

General Information of Target

Target ID LDTP10422
Target Name Terminal nucleotidyltransferase 5A (TENT5A)
Gene Name TENT5A
Gene ID 55603
Synonyms
C6orf37; FAM46A; XTP11; Terminal nucleotidyltransferase 5A; EC 2.7.7.19; HBV X-transactivated gene 11 protein; HBV XAg-transactivated protein 11
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MLPCAAGARGRGAMVVLRAGKKTFLPPLCRAFACRGCQLAPERGAERRDTAPSGVSRFCP
PRKSCHDWIGPPDKYSNLRPVHFYIPENESPLEQKLRKLRQETQEWNQQFWANQNLTFSK
EKEEFIHSRLKTKGLGLRTESGQKATLNAEEMADFYKEFLSKNFQKHMYYNRDWYKRNFA
ITFFMGKVALERIWNKLKQKQKKRSN
Target Bioclass
Enzyme
Family
TENT family
Subcellular location
Cytoplasm
Function
Cytoplasmic non-canonical poly(A) RNA polymerase that catalyzes the transfer of one adenosine molecule from an ATP to an mRNA poly(A) tail bearing a 3'-OH terminal group and participates in the cytoplasmic polyadenylation. Polyadenylates mRNA encoding extracellular matrix constituents and other genes crucial for bone mineralization and during osteoblast mineralization, mainly focuses on ER-targeted mRNAs.
Uniprot ID
Q96IP4
Ensemble ID
ENST00000320172.11
HGNC ID
HGNC:18345

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HCT15 SNV: p.T252N .
JURKAT SNV: p.S221T .
LNCaP clone FGC SNV: p.T252I .
NCIH358 SNV: p.C56F .
NOMO1 Insertion: p.Y339PfsTer19 .
P31FUJ SNV: p.V362A .
REH SNV: p.H359Y .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C214(3.10)  LDD3332  [1]
Acrolein
 Probe Info  		N.A.  LDD0221  [2]
TFBX
 Probe Info  		N.A.  LDD0148  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [2]
 LDCM0107  IAA HeLa N.A.  LDD0221  [2]
 LDCM0022  KB02 A204 C316(2.47)  LDD2252  [1]
 LDCM0023  KB03 A204 C214(3.42); C316(5.69)  LDD2669  [1]
 LDCM0024  KB05 MKN45 C214(3.10)  LDD3332  [1]
 LDCM0109  NEM HeLa N.A.  LDD0224  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Tyrosine-protein kinase SYK (SYK) Tyr protein kinase family P43405
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Ataxin-1 (ATXN1) ATXN1 family P54253
SH2 domain-containing protein 2A (SH2D2A) . Q9NP31

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
3 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255