Details of the Target

General Information of Target

Target ID LDTP09677
Target Name Cholesterol transporter ABCA5 (ABCA5)
Gene Name ABCA5
Gene ID 23461
Synonyms
KIAA1888; Cholesterol transporter ABCA5; EC 7.6.2.-; ATP-binding cassette sub-family A member 5
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSTAIREVGVWRQTRTLLLKNYLIKCRTKKSSVQEILFPLFFLFWLILISMMHPNKKYEE
VPNIELNPMDKFTLSNLILGYTPVTNITSSIMQKVSTDHLPDVIITEEYTNEKEMLTSSL
SKPSNFVGVVFKDSMSYELRFFPDMIPVSSIYMDSRAGCSKSCEAAQYWSSGFTVLQASI
DAAIIQLKTNVSLWKELESTKAVIMGETAVVEIDTFPRGVILIYLVIAFSPFGYFLAIHI
VAEKEKKIKEFLKIMGLHDTAFWLSWVLLYTSLIFLMSLLMAVIATASLLFPQSSSIVIF
LLFFLYGLSSVFFALMLTPLFKKSKHVGIVEFFVTVAFGFIGLMIILIESFPKSLVWLFS
PFCHCTFVIGIAQVMHLEDFNEGASFSNLTAGPYPLIITIIMLTLNSIFYVLLAVYLDQV
IPGEFGLRRSSLYFLKPSYWSKSKRNYEELSEGNVNGNISFSEIIEPVSSEFVGKEAIRI
SGIQKTYRKKGENVEALRNLSFDIYEGQITALLGHSGTGKSTLMNILCGLCPPSDGFASI
YGHRVSEIDEMFEARKMIGICPQLDIHFDVLTVEENLSILASIKGIPANNIIQEVQKVLL
DLDMQTIKDNQAKKLSGGQKRKLSLGIAVLGNPKILLLDEPTAGMDPCSRHIVWNLLKYR
KANRVTVFSTHFMDEADILADRKAVISQGMLKCVGSSMFLKSKWGIGYRLSMYIDKYCAT
ESLSSLVKQHIPGATLLQQNDQQLVYSLPFKDMDKFSGLFSALDSHSNLGVISYGVSMTT
LEDVFLKLEVEAEIDQADYSVFTQQPLEEEMDSKSFDEMEQSLLILSETKAALVSTMSLW
KQQMYTIAKFHFFTLKRESKSVRSVLLLLLIFFTVQIFMFLVHHSFKNAVVPIKLVPDLY
FLKPGDKPHKYKTSLLLQNSADSDISDLISFFTSQNIMVTMINDSDYVSVAPHSAALNVM
HSEKDYVFAAVFNSTMVYSLPILVNIISNYYLYHLNVTETIQIWSTPFFQEITDIVFKIE
LYFQAALLGIIVTAMPPYFAMENAENHKIKAYTQLKLSGLLPSAYWIGQAVVDIPLFFII
LILMLGSLLAFHYGLYFYTVKFLAVVFCLIGYVPSVILFTYIASFTFKKILNTKEFWSFI
YSVAALACIAITEITFFMGYTIATILHYAFCIIIPIYPLLGCLISFIKISWKNVRKNVDT
YNPWDRLSVAVISPYLQCVLWIFLLQYYEKKYGGRSIRKDPFFRNLSTKSKNRKLPEPPD
NEDEDEDVKAERLKVKELMGCQCCEEKPSIMVSNLHKEYDDKKDFLLSRKVKKVATKYIS
FCVKKGEILGLLGPNGAGKSTIINILVGDIEPTSGQVFLGDYSSETSEDDDSLKCMGYCP
QINPLWPDTTLQEHFEIYGAVKGMSASDMKEVISRITHALDLKEHLQKTVKKLPAGIKRK
LCFALSMLGNPQITLLDEPSTGMDPKAKQHMWRAIRTAFKNRKRAAILTTHYMEEAEAVC
DRVAIMVSGQLRCIGTVQHLKSKFGKGYFLEIKLKDWIENLEVDRLQREIQYIFPNASRQ
ESFSSILAYKIPKEDVQSLSQSFFKLEEAKHAFAIEEYSFSQATLEQVFVELTKEQEEED
NSCGTLNSTLWWERTQEDRVVF
Target Bioclass
Enzyme
Family
ABC transporter superfamily, ABCA family
Subcellular location
Golgi apparatus membrane
Function
Cholesterol efflux transporter in macrophages that is responsible for APOAI/high-density lipoproteins (HDL) formation at the plasma membrane under high cholesterol levels and participates in reverse cholesterol transport. May play a role in the processing of autolysosomes.
Uniprot ID
Q8WWZ7
Ensemble ID
ENST00000392676.8
HGNC ID
HGNC:35

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CMK SNV: p.G1403R .
HCT116 SNV: p.N525D .
HT115 SNV: p.E1549Ter .
KYSE30 SNV: p.S149F .
LN18 SNV: p.G1159R .
MDAMB231 SNV: p.S149F .
MDAMB436 SNV: p.W1066C .
MDST8 SNV: p.S387L .
MEWO SNV: p.P1175L .
NB4 SNV: p.I1082L .
NCIH2170 SNV: p.F131L .
NCIH23 SNV: p.K683Ter; p.E858G .
OVK18 SNV: p.A581T .
RL952 Insertion: p.P143SfsTer2 .
SAOS2 SNV: p.P1555S .
SKMM2 SNV: p.K1431R .
TE10 SNV: p.K556N .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
ONAyne
 Probe Info  		N.A.  LDD0273  [1]
DBIA
 Probe Info  		C736(2.12)  LDD3332  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HEK-293T C1513(0.97)  LDD1508  [3]
 LDCM0277  AC18 HEK-293T C1513(0.95)  LDD1516  [3]
 LDCM0279  AC2 HEK-293T C1513(0.85)  LDD1518  [3]
 LDCM0286  AC26 HEK-293T C1513(1.04)  LDD1525  [3]
 LDCM0295  AC34 HEK-293T C1513(0.94)  LDD1534  [3]
 LDCM0304  AC42 HEK-293T C1513(0.84)  LDD1543  [3]
 LDCM0313  AC50 HEK-293T C1513(0.85)  LDD1552  [3]
 LDCM0321  AC58 HEK-293T C1513(0.99)  LDD1560  [3]
 LDCM0405  CL18 HEK-293T C1513(1.10)  LDD1609  [3]
 LDCM0419  CL30 HEK-293T C1513(1.04)  LDD1623  [3]
 LDCM0432  CL42 HEK-293T C1513(0.88)  LDD1636  [3]
 LDCM0445  CL54 HEK-293T C1513(1.24)  LDD1648  [3]
 LDCM0451  CL6 HEK-293T C1513(1.10)  LDD1654  [3]
 LDCM0458  CL66 HEK-293T C1513(1.09)  LDD1661  [3]
 LDCM0471  CL78 HEK-293T C1513(0.98)  LDD1674  [3]
 LDCM0485  CL90 HEK-293T C1513(1.05)  LDD1688  [3]
 LDCM0022  KB02 HuH-1 C736(1.33)  LDD2374  [2]
 LDCM0023  KB03 HuH-1 C736(1.39)  LDD2791  [2]
 LDCM0024  KB05 MKN45 C736(2.12)  LDD3332  [2]

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402