Details of the Target

General Information of Target

Target ID LDTP09663
Target Name Sialidase-4 (NEU4)
Gene Name NEU4
Gene ID 129807
Synonyms
Sialidase-4; EC 3.2.1.18; N-acetyl-alpha-neuraminidase 4
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MGVPRTPSRTVLFERERTGLTYRVPSLLPVPPGPTLLAFVEQRLSPDDSHAHRLVLRRGT
LAGGSVRWGALHVLGTAALAEHRSMNPCPVHDAGTGTVFLFFIAVLGHTPEAVQIATGRN
AARLCCVASRDAGLSWGSARDLTEEAIGGAVQDWATFAVGPGHGVQLPSGRLLVPAYTYR
VDRRECFGKICRTSPHSFAFYSDDHGRTWRCGGLVPNLRSGECQLAAVDGGQAGSFLYCN
ARSPLGSRVQALSTDEGTSFLPAERVASLPETAWGCQGSIVGFPAPAPNRPRDDSWSVGP
GSPLQPPLLGPGVHEPPEEAAVDPRGGQVPGGPFSRLQPRGDGPRQPGPRPGVSGDVGSW
TLALPMPFAAPPQSPTWLLYSHPVGRRARLHMGIRLSQSPLDPRSWTEPWVIYEGPSGYS
DLASIGPAPEGGLVFACLYESGARTSYDEISFCTFSLREVLENVPASPKPPNLGDKPRGC
CWPS
Target Bioclass
Enzyme
Family
Glycosyl hydrolase 33 family
Subcellular location
Mitochondrion inner membrane; Cell membrane
Function
Exo-alpha-sialidase that catalyzes the hydrolytic cleavage of the terminal sialic acid (N-acetylneuraminic acid, Neu5Ac) of a glycan moiety in the catabolism of glycolipids, glycoproteins and oligosacharides. Efficiently hydrolyzes gangliosides including alpha-(2->3)-sialylated GD1a and GM3 and alpha-(2->8)-sialylated GD3. Hydrolyzes poly-alpha-(2->8)-sialylated neural cell adhesion molecule NCAM1 likely at growth cones, suppressing neurite outgrowth in hippocampal neurons. May desialylate sialyl Lewis A and X antigens at the cell surface, down-regulating these glycan epitopes recognized by SELE/E selectin in the initiation of cell adhesion and extravasation. Has sialidase activity toward mucin, fetuin and sialyllactose.
Uniprot ID
Q8WWR8
Ensemble ID
ENST00000325935.10
HGNC ID
HGNC:21328
ChEMBL ID
CHEMBL4174

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A2058 SNV: p.H314Y .
CHL1 SNV: p.D475E .
COLO800 SNV: p.G312E .
CORL88 SNV: p.A241S .
DU145 SNV: p.G352V .
HCT116 Insertion: p.G33RfsTer15 .
JURKAT SNV: p.P29L; p.T76I .
LOXIMVI Deletion: p.F187LfsTer136 .
MEC1 Substitution: p.G234F .
MOLT4 Deletion: p.C480AfsTer84
SNV: p.D48N		 .
NCIH2286 SNV: p.P300H .
NCIH23 SNV: p.V266L .
NCIH716 SNV: p.L304P .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C224(2.95)  LDD2359  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0022  KB02 HMCB C224(2.95)  LDD2359  [1]
 LDCM0023  KB03 A-375 C224(1.69)  LDD2672  [1]
 LDCM0024  KB05 HMCB C224(2.88)  LDD3193  [1]

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840