Details of the Target

General Information of Target

Target ID LDTP09648
Target Name Ankyrin repeat, SAM and basic leucine zipper domain-containing protein 1 (ASZ1)
Gene Name ASZ1
Gene ID 136991
Synonyms
ALP1; ANKL1; C7orf7; GASZ; Ankyrin repeat, SAM and basic leucine zipper domain-containing protein 1; Ankyrin-like protein 1; Germ cell-specific ankyrin, SAM and basic leucine zipper domain-containing protein
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAASALRGLPVAGGGESSESEDDGWEIGYLDRTSQKLKRLLPIEEKKEKFKKAMTIGDVS
LVQELLDSGISVDSNFQYGWTPLMYAASVANAELVRVLLDRGANASFEKDKQSILITACS
AHGSEEQILKCVELLLSRNADPNVACRRLMTPIMYAARDGHTQVVALLVAHGAEVNTQDE
NGYTALTWAARQGHKNIVLKLLELGANKMLQTKDGKMPSEIAKRNKHHEIFNLLSFTLNP
LEGKLQQLTKEDTICKILTTDSDREKDHIFSSYTAFGDLEVFLHGLGLEHMTDLLKERDI
TLRHLLTMREDEFTKNGITSKDQQKILAALKELQVEEIQFGELSEETKLEISGDEFLNFL
LKLNKQCGHLITAVQNVITELPVNSQKITLEWASPQNFTSVCEELVNNVEDLSEKVCKLK
DLIQKLQNERENDPTHIQLREEVSTWNSRILKRTAITICGFGFLLFICKLTFQRK
Target Bioclass
Other
Subcellular location
Cytoplasm
Function
Plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Its association with pi-bodies suggests a participation in the primary piRNAs metabolic process. Required prior to the pachytene stage to facilitate the production of multiple types of piRNAs, including those associated with repeats involved in the regulation of retrotransposons. May act by mediating protein-protein interactions during germ cell maturation.
Uniprot ID
Q8WWH4
Ensemble ID
ENST00000284629.7
HGNC ID
HGNC:1350

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
G402 SNV: p.Q63L .
MCC13 SNV: p.H227Y .
MDAMB436 SNV: p.K250R .
MELJUSO Deletion: p.L6PfsTer30 .
MOLT4 SNV: p.E45K .
NCIH716 Deletion: p.L6PfsTer30 .
PC9 SNV: p.S271I .
RKO SNV: p.Q473K .
SNU869 SNV: p.Q386Ter .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IA-alkyne
 Probe Info  		C367(0.54)  LDD2182  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0573  Fragment11 Ramos C367(2.83)  LDD2190  [1]
 LDCM0576  Fragment14 Ramos C367(1.08)  LDD2193  [1]
 LDCM0586  Fragment28 Ramos C367(0.99)  LDD2198  [1]
 LDCM0566  Fragment4 Ramos C367(0.98)  LDD2184  [1]
 LDCM0569  Fragment7 Ramos C367(0.51)  LDD2186  [1]
 LDCM0022  KB02 Ramos C367(0.54)  LDD2182  [1]
 LDCM0023  KB03 Ramos C367(2.75)  LDD2183  [1]
 LDCM0024  KB05 Ramos C367(3.81)  LDD2185  [1]

References

1 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578