Details of the Target

General Information of Target

Target ID LDTP09546
Target Name Protein DDI1 homolog 1 (DDI1)
Gene Name DDI1
Gene ID 414301
Synonyms
Protein DDI1 homolog 1; EC 3.4.23.-
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MLITVYCVRRDLSEVTFSLQVSPDFELRNFKVLCEAESRVPVEEIQIIHMERLLIEDHCS
LGSYGLKDGDIVVLLQKDNVGPRAPGRAPNQPRVDFSGIAVPGTSSSRPQHPGQQQQRTP
AAQRSQGLASGEKVAGLQGLGSPALIRSMLLSNPHDLSLLKERNPPLAEALLSGSLETFS
QVLMEQQREKALREQERLRLYTADPLDREAQAKIEEEIRQQNIEENMNIAIEEAPESFGQ
VTMLYINCKVNGHPLKAFVDSGAQMTIMSQACAERCNIMRLVDRRWAGVAKGVGTQRIIG
RVHLAQIQIEGDFLQCSFSILEDQPMDMLLGLDMLRRHQCSIDLKKNVLVIGTTGTQTYF
LPEGELPLCSRMVSGQDESSDKEITHSVMDSGRKEH
Target Bioclass
Enzyme
Family
DDI1 family
Function
Probable aspartic protease (Probable). Seems to act as a proteasomal shuttle which links the proteasome and replication fork proteins like RTF2 (Probable). Required, with DDI2, for cellular survival following replication stress. Together or redudantly with DDI2, removes RTF2 from stalled forks to allow cell cycle progression after replication stress and maintains genome integrity.
Uniprot ID
Q8WTU0
Ensemble ID
ENST00000302259.5
HGNC ID
HGNC:18961

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C7(2.20)  LDD3365  [1]
Methacrolein
 Probe Info  		N.A.  LDD0218  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0022  KB02 A-375 C7(1.36)  LDD2255  [1]
 LDCM0023  KB03 A-375 C7(1.71)  LDD2672  [1]
 LDCM0024  KB05 OVCAR-3 C7(2.20)  LDD3365  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 8 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cytoglobin (CYGB) Globin family Q8WWM9
Ribonuclease P protein subunit p25 (RPP25) Histone-like Alba family Q9BUL9
Ubiquitin carboxyl-terminal hydrolase 2 (USP2) Peptidase C19 family O75604
Proteasome subunit beta type-1 (PSMB1) Peptidase T1B family P20618
L-serine dehydratase/L-threonine deaminase (SDS) Serine/threonine dehydratase family P20132
TNF receptor-associated factor 2 (TRAF2) TNF receptor-associated factor family Q12933
Ubiquitin-conjugating enzyme E2 K (UBE2K) Ubiquitin-conjugating enzyme family P61086
F-box only protein 50 (NCCRP1) . Q6ZVX7
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Krueppel-like factor 11 (KLF11) Sp1 C2H2-type zinc-finger protein family O14901
Cytokine and receptor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
C-C motif chemokine 28 (CCL28) Intercrine beta (chemokine CC) family Q9NRJ3
Other
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein FAM161B (FAM161B) FAM161 family Q96MY7
Axonemal dynein light intermediate polypeptide 1 (DNALI1) Inner dynein arm light chain family O14645
Tektin-3 (TEKT3) Tektin family Q9BXF9
G patch domain and ankyrin repeat-containing protein 1 (GPANK1) . O95872
Kelch-like protein 42 (KLHL42) . Q9P2K6
Ral GTPase-activating protein subunit alpha-2 (RALGAPA2) . Q2PPJ7

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.