Details of the Target

General Information of Target

Target ID LDTP09486
Target Name 2-phosphoxylose phosphatase 1 (PXYLP1)
Gene Name PXYLP1
Gene ID 92370
Synonyms
ACPL2; HEL124; XYLP; 2-phosphoxylose phosphatase 1; EC 3.1.3.-; Acid phosphatase-like protein 2; Xylosyl phosphatase; epididymis luminal protein 124
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MLFRNRFLLLLALAALLAFVSLSLQFFHLIPVSTPKNGMSSKSRKRIMPDPVTEPPVTDP
VYEALLYCNIPSVAERSMEGHAPHHFKLVSVHVFIRHGDRYPLYVIPKTKRPEIDCTLVA
NRKPYHPKLEAFISHMSKGSGASFESPLNSLPLYPNHPLCEMGELTQTGVVQHLQNGQLL
RDIYLKKHKLLPNDWSADQLYLETTGKSRTLQSGLALLYGFLPDFDWKKIYFRHQPSALF
CSGSCYCPVRNQYLEKEQRRQYLLRLKNSQLEKTYGEMAKIVDVPTKQLRAANPIDSMLC
HFCHNVSFPCTRNGCVDMEHFKVIKTHQIEDERERREKKLYFGYSLLGAHPILNQTIGRM
QRATEGRKEELFALYSAHDVTLSPVLSALGLSEARFPRFAARLIFELWQDREKPSEHSVR
ILYNGVDVTFHTSFCQDHHKRSPKPMCPLENLVRFVKRDMFVALGGSGTNYYDACHREGF
Target Bioclass
Enzyme
Family
Histidine acid phosphatase family
Subcellular location
Golgi apparatus membrane
Function
Responsible for the 2-O-dephosphorylation of xylose in the glycosaminoglycan-protein linkage region of proteoglycans thereby regulating the amount of mature glycosaminoglycan (GAG) chains. Sulfated glycosaminoglycans (GAGs), including heparan sulfate and chondroitin sulfate, are synthesized on the so-called common GAG-protein linkage region (GlcUAbeta1-3Galbeta1-3Galbeta1-4Xylbeta1-O-Ser) of core proteins, which is formed by the stepwise addition of monosaccharide residues by the respective specific glycosyltransferases. Xylose 2-O-dephosphorylation during completion of linkage region formation is a prerequisite for the initiation and efficient elongation of the repeating disaccharide region of GAG chains.
Uniprot ID
Q8TE99
Ensemble ID
ENST00000286353.9
HGNC ID
HGNC:26303

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CAMA1 SNV: p.D224G .
HS936T SNV: p.P31S .
SW620 SNV: p.K256N .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C116(1.03)  LDD1508  [1]
SF
 Probe Info  		N.A.  LDD0028  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HEK-293T C116(1.03)  LDD1508  [1]
 LDCM0226  AC11 HEK-293T C116(1.02)  LDD1509  [1]
 LDCM0277  AC18 HEK-293T C116(0.95)  LDD1516  [1]
 LDCM0278  AC19 HEK-293T C116(1.12)  LDD1517  [1]
 LDCM0279  AC2 HEK-293T C116(0.96)  LDD1518  [1]
 LDCM0286  AC26 HEK-293T C116(0.86)  LDD1525  [1]
 LDCM0287  AC27 HEK-293T C116(0.96)  LDD1526  [1]
 LDCM0290  AC3 HEK-293T C116(1.09)  LDD1529  [1]
 LDCM0295  AC34 HEK-293T C116(1.01)  LDD1534  [1]
 LDCM0296  AC35 HEK-293T C116(0.99)  LDD1535  [1]
 LDCM0304  AC42 HEK-293T C116(0.94)  LDD1543  [1]
 LDCM0305  AC43 HEK-293T C116(1.09)  LDD1544  [1]
 LDCM0313  AC50 HEK-293T C116(0.98)  LDD1552  [1]
 LDCM0314  AC51 HEK-293T C116(1.01)  LDD1553  [1]
 LDCM0321  AC58 HEK-293T C116(1.02)  LDD1560  [1]
 LDCM0322  AC59 HEK-293T C116(0.99)  LDD1561  [1]
 LDCM0020  ARS-1620 HCC44 C241(1.40); C247(1.40)  LDD2171  [3]
 LDCM0405  CL18 HEK-293T C116(1.06)  LDD1609  [1]
 LDCM0406  CL19 HEK-293T C116(1.31)  LDD1610  [1]
 LDCM0419  CL30 HEK-293T C116(1.09)  LDD1623  [1]
 LDCM0420  CL31 HEK-293T C116(1.25)  LDD1624  [1]
 LDCM0432  CL42 HEK-293T C116(1.17)  LDD1636  [1]
 LDCM0433  CL43 HEK-293T C116(1.12)  LDD1637  [1]
 LDCM0445  CL54 HEK-293T C116(1.11)  LDD1648  [1]
 LDCM0446  CL55 HEK-293T C116(1.19)  LDD1649  [1]
 LDCM0451  CL6 HEK-293T C116(1.08)  LDD1654  [1]
 LDCM0458  CL66 HEK-293T C116(1.13)  LDD1661  [1]
 LDCM0459  CL67 HEK-293T C116(1.17)  LDD1662  [1]
 LDCM0462  CL7 HEK-293T C116(1.29)  LDD1665  [1]
 LDCM0471  CL78 HEK-293T C116(1.17)  LDD1674  [1]
 LDCM0472  CL79 HEK-293T C116(1.03)  LDD1675  [1]
 LDCM0485  CL90 HEK-293T C116(1.07)  LDD1688  [1]
 LDCM0486  CL91 HEK-293T C116(1.04)  LDD1689  [1]
 LDCM0021  THZ1 HCT 116 C241(1.40); C247(1.40)  LDD2173  [3]

References

1 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
2 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
3 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.