Details of the Target

General Information of Target

Target ID LDTP09337
Target Name SH3KBP1-binding protein 1 (SHKBP1)
Gene Name SHKBP1
Gene ID 92799
Synonyms
SB1; SH3KBP1-binding protein 1; SETA-binding protein 1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAAAATAAEGVPSRGPPGEVIHLNVGGKRFSTSRQTLTWIPDSFFSSLLSGRISTLKDET
GAIFIDRDPTVFAPILNFLRTKELDPRGVHGSSLLHEAQFYGLTPLVRRLQLREELDRSS
CGNVLFNGYLPPPVFPVKRRNRHSLVGPQQLGGRPAPVRRSNTMPPNLGNAGLLGRMLDE
KTPPSPSGQPEEPGMVRLVCGHHNWIAVAYTQFLVCYRLKEASGWQLVFSSPRLDWPIER
LALTARVHGGALGEHDKMVAAATGSEILLWALQAEGGGSEIGVFHLGVPVEALFFVGNQL
IATSHTGRIGVWNAVTKHWQVQEVQPITSYDAAGSFLLLGCNNGSIYYVDVQKFPLRMKD
NDLLVSELYRDPAEDGVTALSVYLTPKTSDSGNWIEIAYGTSSGGVRVIVQHPETVGSGP
QLFQTFTVHRSPVTKIMLSEKHLISVCADNNHVRTWSVTRFRGMISTQPGSTPLASFKIL
ALESADGHGGCSAGNDIGPYGERDDQQVFIQKVVPSASQLFVRLSSTGQRVCSVRSVDGS
PTTAFTVLECEGSRRLGSRPRRYLLTGQANGSLAMWDLTTAMDGLGQAPAGGLTEQELME
QLEHCELAPPAPSAPSWGCLPSPSPRISLTSLHSASSNTSLSGHRGSPSPPQAEARRRGG
GSFVERCQELVRSGPDLRRPPTPAPWPSSGLGTPLTPPKMKLNETSF
Target Bioclass
Other
Family
KCTD3 family
Subcellular location
Lysosome
Function
Inhibits CBL-SH3KBP1 complex mediated down-regulation of EGFR signaling by sequestration of SH3KBP1. Binds to SH3KBP1 and prevents its interaction with CBL and inhibits translocation of SH3KBP1 to EGFR containing vesicles upon EGF stimulation.
Uniprot ID
Q8TBC3
Ensemble ID
ENST00000291842.10
HGNC ID
HGNC:19214

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
786O SNV: p.D362G .
A431 SNV: p.L620V .
HCT116 SNV: p.C532R .
IGR1 Substitution: p.A608V DBIA    Probe Info 
Ishikawa (Heraklio) 02 ER SNV: p.V196L .
JURKAT SNV: p.R554W Compound 10    Probe Info 
LN18 SNV: p.R460L DBIA    Probe Info 
LS180 SNV: p.G61R .
MEWO SNV: p.P612S .
MFE319 SNV: p.H442R DBIA    Probe Info 
MKN45 SNV: p.S492N .
OV90 SNV: p.A156D .
SNU1 SNV: p.R430H .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 11 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0402  [1]
DBIA
 Probe Info  		C667(7.37)  LDD0209  [2]
IA-alkyne
 Probe Info  		C550(5.82)  LDD1703  [3]
Acrolein
 Probe Info  		N.A.  LDD0221  [4]
Lodoacetamide azide
 Probe Info  		C550(0.00); C491(0.00)  LDD0037  [5]
NAIA_4
 Probe Info  		C491(0.00); C550(0.00)  LDD2226  [6]
Compound 10
 Probe Info  		N.A.  LDD2216  [7]
Compound 11
 Probe Info  		N.A.  LDD2213  [7]
ENE
 Probe Info  		N.A.  LDD0006  [8]
IPM
 Probe Info  		N.A.  LDD0005  [8]
VSF
 Probe Info  		N.A.  LDD0007  [8]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0107  IAA HeLa N.A.  LDD0221  [4]
 LDCM0022  KB02 HEK-293T C491(1.06)  LDD1492  [9]
 LDCM0023  KB03 Jurkat C667(7.37)  LDD0209  [2]
 LDCM0024  KB05 MEWO C550(19.62)  LDD3319  [10]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cullin-3 (CUL3) Cullin family Q13618
Other
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
SH3KBP1-binding protein 1 (SHKBP1) KCTD3 family Q8TBC3
Latexin (LXN) Protease inhibitor I47 (latexin) family Q9BS40
U4/U6 small nuclear ribonucleoprotein Prp3 (PRPF3) . O43395

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
3 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
4 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
5 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
6 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
7 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279
8 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
9 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
10 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840