Details of the Target

General Information of Target

Target ID LDTP09173
Target Name Carbohydrate sulfotransferase 13 (CHST13)
Gene Name CHST13
Gene ID 166012
Synonyms
Carbohydrate sulfotransferase 13; EC 2.8.2.5; Chondroitin 4-O-sulfotransferase 3; Chondroitin 4-sulfotransferase 3; C4ST-3; C4ST3
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MGRRCCRRRVLAAACLGAALLLLCAAPRSLRPAFGNRALGSSWLGGEKRSPLQKLYDLDQ
DPRSTLAKVHRQRRDLLNSACSRHSRRQRLLQPEDLRHVLVDDAHGLLYCYVPKVACTNW
KRVLLALSGQARGDPRAISAQEAHAPGRLPSLADFSPAEINRRLRAYLAFLFVREPFERL
ASAYRNKLARPYSAAFQRRYGARIVQRLRPRALPDARARGHDVRFAEFLAYLLDPRTRRE
EPFNEHWERAHALCHPCRLRYDVVGKFETLAEDAAFVLGLAGASDLSFPGPPRPRGAAAS
RDLAARLFRDISPFYQRRLFDLYKMDFLLFNYSAPSYLRLL
Target Bioclass
Enzyme
Family
Sulfotransferase 2 family
Subcellular location
Golgi apparatus membrane
Function
Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Transfers sulfate to the C4 hydroxyl of beta1,4-linked GalNAc that is substituted with a beta-linked glucuronic acid at the C-3 hydroxyl. No activity toward dermatan.
Uniprot ID
Q8NET6
Ensemble ID
ENST00000319340.7
HGNC ID
HGNC:21755

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CAL33 SNV: p.P191L .
CCK81 SNV: p.Y192C .
HEC1 SNV: p.V277M .
RVH421 Deletion: p.R136PfsTer151
SNV: p.A137V		 .
SKMEL28 SNV: p.D302Y .
SUPT1 SNV: p.A252V .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IA-alkyne
 Probe Info  		N.A.  LDD2241  [1]
Compound 10
 Probe Info  		N.A.  LDD2216  [2]

References

1 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
2 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279