Details of the Target

General Information of Target

Target ID LDTP09159
Target Name Neuroguidin (NGDN)
Gene Name NGDN
Gene ID 25983
Synonyms
C14orf120; Neuroguidin; Centromere accumulated nuclear protein 1; CANu1; EIF4E-binding protein
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAALGVLESDLPSAVTLLKNLQEQVMAVTAQVKSLTQKVQAGAYPTEKGLSFLEVKDQLL
LMYLMDLTHLILDKASGGSLQGHDAVLRLVEIRTVLEKLRPLDQKLKYQIDKLIKTAVTG
SLSENDPLRFKPHPSNMMSKLSSEDEEEDEAEDDQSEASGKKSVKGVSKKYVPPRLVPVH
YDETEAEREKKRLERAKRRALSSSVIRELKEQYSDAPEEIRDARHPHVTRQSQEDQHRIN
YEESMMVRLSVSKREKGRRKRANVMSSQLHSLTHFSDISALTGGTVHLDEDQNPIKKRKK
IPQKGRKKKGFRRRR
Target Bioclass
Other
Family
SAS10 family
Subcellular location
Nucleus
Function
Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. Its dissociation from the complex determines the transition from state pre-A1 to state pre-A1*. Inhibits mRNA translation in a cytoplasmic polyadenylation element (CPE)-dependent manner.
Uniprot ID
Q8NEJ9
Ensemble ID
ENST00000397154.7
HGNC ID
HGNC:20271

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HCT15 SNV: p.S76A .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
Probe 1
 Probe Info  		Y213(14.42)  LDD3495  [1]
SF
 Probe Info  		N.A.  LDD0028  [2]
Acrolein
 Probe Info  		N.A.  LDD0217  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [3]
 LDCM0107  IAA HeLa N.A.  LDD0221  [3]
 LDCM0109  NEM HeLa H83(0.00); H180(0.00)  LDD0223  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Casein kinase II subunit beta (CSNK2B) Casein kinase 2 subunit beta family P67870
Other
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein AATF (AATF) AATF family Q9NY61
Nucleolar MIF4G domain-containing protein 1 (NOM1) CWC22 family Q5C9Z4
Microfibrillar-associated protein 1 (MFAP1) MFAP1 family P55081

References

1 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
2 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
3 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.