General Information of Target

Target ID LDTP09147
Target Name Cytoplasmic polyadenylation element-binding protein 3 (CPEB3)
Gene Name CPEB3
Gene ID 22849
Synonyms
KIAA0940; Cytoplasmic polyadenylation element-binding protein 3; CPE-BP3; CPE-binding protein 3; hCPEB-3
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MQDDLLMDKSKTQPQPQQQQRQQQQPQPESSVSEAPSTPLSSETPKPEENSAVPALSPAA
APPAPNGPDKMQMESPLLPGLSFHQPPQQPPPPQEPAAPGASLSPSFGSTWSTGTTNAVE
DSFFQGITPVNGTMLFQNFPHHVNPVFGGTFSPQIGLAQTQHHQQPPPPAPAPQPAQPAQ
PPQAQPPQQRRSPASPSQAPYAQRSAAAAYGHQPIMTSKPSSSSAVAAAAAAAAASSASS
SWNTHQSVNAAWSAPSNPWGGLQAGRDPRRAVGVGVGVGVGVPSPLNPISPLKKPFSSNV
IAPPKFPRAAPLTSKSWMEDNAFRTDNGNNLLPFQDRSRPYDTFNLHSLENSLMDMIRTD
HEPLKGKHYPPSGPPMSFADIMWRNHFAGRMGINFHHPGTDNIMALNNAFLDDSHGDQAL
SSGLSSPTRCQNGERVERYSRKVFVGGLPPDIDEDEITASFRRFGPLVVDWPHKAESKSY
FPPKGYAFLLFQEESSVQALIDACLEEDGKLYLCVSSPTIKDKPVQIRPWNLSDSDFVMD
GSQPLDPRKTIFVGGVPRPLRAVELAMIMDRLYGGVCYAGIDTDPELKYPKGAGRVAFSN
QQSYIAAISARFVQLQHNDIDKRVEVKPYVLDDQMCDECQGTRCGGKFAPFFCANVTCLQ
YYCEYCWASIHSRAGREFHKPLVKEGGDRPRHVPFRWS
Target Bioclass
Other
Family
RRM CPEB family
Subcellular location
Cytoplasm
Function
Sequence-specific RNA-binding protein which acts as a translational repressor in the basal unstimulated state but, following neuronal stimulation, acts as a translational activator. In contrast to CPEB1, does not bind to the cytoplasmic polyadenylation element (CPE), a uridine-rich sequence element within the mRNA 3'-UTR, but binds to a U-rich loop within a stem-loop structure. Required for the consolidation and maintenance of hippocampal-based long term memory. In the basal state, binds to the mRNA 3'-UTR of the glutamate receptors GRIA2/GLUR2 mRNA and negatively regulates their translation. Also represses the translation of DLG4, GRIN1, GRIN2A and GRIN2B. When activated, acts as a translational activator of GRIA1 and GRIA2. In the basal state, suppresses SUMO2 translation but activates it following neuronal stimulation. Binds to the 3'-UTR of TRPV1 mRNA and represses TRPV1 translation which is required to maintain normal thermoception. Binds actin mRNA, leading to actin translational repression in the basal state and to translational activation following neuronal stimulation. Negatively regulates target mRNA levels by binding to TOB1 which recruits CNOT7/CAF1 to a ternary complex and this leads to target mRNA deadenylation and decay. In addition to its role in translation, binds to and inhibits the transcriptional activation activity of STAT5B without affecting its dimerization or DNA-binding activity. This, in turn, represses transcription of the STAT5B target gene EGFR which has been shown to play a role in enhancing learning and memory performance. In contrast to CPEB1, CPEB2 and CPEB4, not required for cell cycle progression.
Uniprot ID
Q8NE35
Ensemble ID
ENST00000265997.5
HGNC ID
HGNC:21746

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
8305C SNV: p.E437Q DBIA    Probe Info 
A2780 Insertion: p.G592VfsTer28
SNV: p.G592C
DBIA    Probe Info 
CAL33 SNV: p.W252C .
FADU SNV: p.R528Ter .
HCT116 SNV: p.P178S .
HCT15 SNV: p.Y662H .
HT115 SNV: p.F537C .
IGR37 SNV: p.A238V DBIA    Probe Info 
NCIH1793 SNV: p.A35S DBIA    Probe Info 
OCIAML2 SNV: p.R270L .
OV90 SNV: p.N330T .
RKO SNV: p.S609N DBIA    Probe Info 
SW480 SNV: p.N618I .
SW756 SNV: p.V443M .
TE10 SNV: p.L500F DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info 
C850(1.96)  LDD3314  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0022  KB02 22RV1 C850(1.42)  LDD2243  [1]
 LDCM0023  KB03 22RV1 C850(1.50)  LDD2660  [1]
 LDCM0024  KB05 IGR37 C850(1.96)  LDD3314  [1]

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840