Details of the Target

General Information of Target

Target ID LDTP09139
Target Name PH and SEC7 domain-containing protein 4 (PSD4)
Gene Name PSD4
Gene ID 23550
Synonyms
EFA6B; TIC; PH and SEC7 domain-containing protein 4; Exchange factor for ADP-ribosylation factor guanine nucleotide factor 6 B; Exchange factor for ARF6 B; Pleckstrin homology and SEC7 domain-containing protein 4; Telomeric of interleukin-1 cluster protein
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MMGDYRLPDHPQPMEILNLYLGDSLEPHPGECPRETCSHEDPPEPFEEQTWATDPPEPTR
QNVPPWGSGVELTHLGSWVHQDGLEPCQEQTRATDPPESTRQDAPPWGSGVELTHLGSPS
AQREHRQNTASPGSPVNSHLPGSPKQNRSTSTQVVFWAGILQAQMCVLDLEEELEKTEGL
KAGLKCCLPTPPVDLPGDTGLHSSPPENEDSGEDSSEPEGEGQAWLREGTPDSSPQWGAE
EESMFFSNPLFLASPCSENSASGECFSWGASDSHAGVRTGPESPATLEPPLPEDTVLWEL
ESEPDLGDGAAISGHCTPPFPVPIYKPHSICWASVAAAEGAPAAPPGHGESEGDRLGPAP
SAAPCVDEALTWESGCVGSDLGPAAHPVQPWASLSPEGWQRGGPFWPQVTLNSQDRDERE
GGHPQESLPCTLAPCPWRSPASSPEPSSPESESRGPGPRPSPASSQEGSPQLQHHSSGIL
PKWTLDASQSSLLETDGEQPSSLKKKEAGEAPKPGEEVKSEGTARPAETGDVQPDIHLTS
AEHENLRTPMNSSWLPGSPMPQAQSPEEGQRPPAGDKLANGVRNNKVAWNLASRLYRLEG
FRKSEVAAYLQKNNDFSRAVAEEYLSFFQFGGQSLDRALRSFLQALVLSGETQERERILY
QFSRRFHHCNPGIFPSVDSVHTLTCAIMLLNTDLHGQNIGKSMSCQEFITNLNGLRDGGN
FPKELLKALYWSIRSEKLEWAVDEEDTARPEKAQPSLPAGKMSKPFLQLAQDPTVPTYKQ
GILARKMHQDADGKKTPWGKRGWKMFHTLLRGMVLYFLKQGEDHCLEGESLVGQMVDEPV
GVHHSLATPATHYTKKPHVFQLRTADWRLYLFQAPTAKEMSSWIARINLAAATHSAPPFP
AAVGSQRRFVRPILPVGPAQSSLEEQHRSHENCLDAAADDLLDLQRNLPERRGRGRELEE
HRLRKEYLEYEKTRYETYVQLLVARLHCPSDALDLWEEQLGREAGGTREPKLSLKKSHSS
PSLHQDEAPTTAKVKRNISERRTYRKIIPKRNRNQL
Target Bioclass
Other
Subcellular location
Cell membrane
Function
Guanine nucleotide exchange factor for ARF6 and ARL14/ARF7. Through ARL14 activation, controls the movement of MHC class II-containing vesicles along the actin cytoskeleton in dendritic cells. Involved in membrane recycling. Interacts with several phosphatidylinositol phosphate species, including phosphatidylinositol 3,4-bisphosphate, phosphatidylinositol 3,5-bisphosphate and phosphatidylinositol 4,5-bisphosphate.
Uniprot ID
Q8NDX1
Ensemble ID
ENST00000245796.11
HGNC ID
HGNC:19096

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
22RV1 SNV: p.G718D .
CASKI SNV: p.E299Ter .
COLO792 SNV: p.E35K .
COLO800 SNV: p.L941V .
G415 SNV: p.M880I .
HCT15 SNV: p.P231T .
HEC1B SNV: p.E221D .
ICC3 SNV: p.E494Ter .
JURKAT SNV: p.C685Y .
KMS12BM SNV: p.A433V .
LU65 SNV: p.P42A .
MEWO SNV: p.K761N .
MFE319 SNV: p.Q653R .
NCIH1299 SNV: p.L1012H .
OCUM1 Insertion: . .
SKMEL5 SNV: p.P556S .
SW756 SNV: p.Q834H .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C435(25.42)  LDD0209  [1]
4-Iodoacetamidophenylacetylene
 Probe Info  		C988(0.00); C933(0.00)  LDD0038  [2]
IA-alkyne
 Probe Info  		C988(0.00); C933(0.00); C705(0.00)  LDD0036  [2]
Lodoacetamide azide
 Probe Info  		C988(0.00); C933(0.00); C705(0.00)  LDD0037  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0625  F8 Ramos C988(0.30)  LDD2187  [3]
 LDCM0572  Fragment10 Ramos C988(0.58)  LDD2189  [3]
 LDCM0573  Fragment11 Ramos C988(1.07)  LDD2190  [3]
 LDCM0574  Fragment12 Ramos C988(0.66)  LDD2191  [3]
 LDCM0575  Fragment13 Ramos C988(0.69)  LDD2192  [3]
 LDCM0576  Fragment14 Ramos C988(0.96)  LDD2193  [3]
 LDCM0579  Fragment20 Ramos C988(0.54)  LDD2194  [3]
 LDCM0580  Fragment21 Ramos C988(0.60)  LDD2195  [3]
 LDCM0582  Fragment23 Ramos C988(0.84)  LDD2196  [3]
 LDCM0588  Fragment30 Ramos C988(0.90)  LDD2199  [3]
 LDCM0589  Fragment31 Ramos C988(0.58)  LDD2200  [3]
 LDCM0590  Fragment32 Ramos C988(0.82)  LDD2201  [3]
 LDCM0468  Fragment33 Ramos C988(1.62)  LDD2202  [3]
 LDCM0566  Fragment4 Ramos C988(0.70)  LDD2184  [3]
 LDCM0614  Fragment56 Ramos C988(0.76)  LDD2205  [3]
 LDCM0569  Fragment7 Ramos C988(0.53)  LDD2186  [3]
 LDCM0571  Fragment9 Ramos C988(0.48)  LDD2188  [3]
 LDCM0022  KB02 T cell-activated C435(20.00)  LDD1708  [4]
 LDCM0023  KB03 Jurkat C435(25.42)  LDD0209  [1]
 LDCM0024  KB05 OCI-Ly3 C705(1.57)  LDD3372  [5]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Low density lipoprotein receptor adapter protein 1 (LDLRAP1) . Q5SW96

References

1 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
2 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
3 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
4 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
5 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840