Details of the Target

General Information of Target

Target ID LDTP09052
Target Name Equilibrative nucleobase transporter 1 (SLC43A3)
Gene Name SLC43A3
Gene ID 29015
Synonyms
ENBT1; Equilibrative nucleobase transporter 1; Protein FOAP-13; Solute carrier family 43 member 3
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAGQGLPLHVATLLTGLLECLGFAGVLFGWPSLVFVFKNEDYFKDLCGPDAGPIGNATGQ
ADCKAQDERFSLIFTLGSFMNNFMTFPTGYIFDRFKTTVARLIAIFFYTTATLIIAFTSA
GSAVLLFLAMPMLTIGGILFLITNLQIGNLFGQHRSTIITLYNGAFDSSSAVFLIIKLLY
EKGISLRASFIFISVCSTWHVARTFLLMPRGHIPYPLPPNYSYGLCPGNGTTKEEKETAE
HENRELQSKEFLSAKEETPGAGQKQELRSFWSYAFSRRFAWHLVWLSVIQLWHYLFIGTL
NSLLTNMAGGDMARVSTYTNAFAFTQFGVLCAPWNGLLMDRLKQKYQKEARKTGSSTLAV
ALCSTVPSLALTSLLCLGFALCASVPILPLQYLTFILQVISRSFLYGSNAAFLTLAFPSE
HFGKLFGLVMALSAVVSLLQFPIFTLIKGSLQNDPFYVNVMFMLAILLTFFHPFLVYREC
RTWKESPSAIA
Target Bioclass
Transporter and channel
Family
SLC43A transporter (TC 2.A.1.44) family
Subcellular location
Basolateral cell membrane
Function
Sodium-independent purine-selective nucleobase transporter which mediates the equilibrative transport of extracellular purine nucleobases such as adenine, guanine and hypoxanthine. May regulate fatty acid (FA) transport in adipocytes, acting as a positive regulator of FA efflux and as a negative regulator of FA uptake.; [Isoform 1]: Sodium-independent purine-selective nucleobase transporter which mediates the equilibrative transport of extracellular purine nucleobase adenine. Mediates the influx and efflux of the purine nucleobase analog drug 6-mercaptopurine across the membrane.; [Isoform 2]: Sodium-independent purine-selective nucleobase transporter which mediates the equilibrative transport of extracellular purine nucleobase adenine. Mediates the influx and efflux of the purine nucleobase analog drug 6-mercaptopurine across the membrane.
Uniprot ID
Q8NBI5
Ensemble ID
ENST00000352187.5
HGNC ID
HGNC:17466

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CCK81 Deletion: p.H212TfsTer77 .
HCC1419 SNV: p.M80T .
SKMEL2 SNV: p.Q153L .
SNGM Deletion: p.H212TfsTer77 .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K249(9.09); K255(10.00); K264(4.74); K345(2.28)  LDD0277  [1]
ONAyne
 Probe Info  		K249(9.09); K264(9.58)  LDD0275  [1]
DBIA
 Probe Info  		C344(0.88)  LDD3413  [2]
IA-alkyne
 Probe Info  		C226(2.70)  LDD2182  [3]
IPM
 Probe Info  		N.A.  LDD0147  [4]
TFBX
 Probe Info  		N.A.  LDD0148  [4]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STS-1
 Probe Info  		N.A.  LDD0136  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0625  F8 Ramos C226(1.54)  LDD2187  [3]
 LDCM0572  Fragment10 Ramos C226(2.76)  LDD2189  [3]
 LDCM0573  Fragment11 Ramos C226(2.12)  LDD2190  [3]
 LDCM0575  Fragment13 Ramos C226(1.07)  LDD2192  [3]
 LDCM0576  Fragment14 Ramos C226(1.28)  LDD2193  [3]
 LDCM0580  Fragment21 Ramos C226(1.13)  LDD2195  [3]
 LDCM0582  Fragment23 Ramos C226(0.96)  LDD2196  [3]
 LDCM0578  Fragment27 Ramos C226(0.85)  LDD2197  [3]
 LDCM0586  Fragment28 Ramos C226(1.11)  LDD2198  [3]
 LDCM0588  Fragment30 Ramos C226(0.63)  LDD2199  [3]
 LDCM0589  Fragment31 Ramos C226(0.66)  LDD2200  [3]
 LDCM0590  Fragment32 Ramos C226(1.98)  LDD2201  [3]
 LDCM0468  Fragment33 Ramos C226(0.61)  LDD2202  [3]
 LDCM0566  Fragment4 Ramos C226(2.52)  LDD2184  [3]
 LDCM0610  Fragment52 Ramos C226(0.68)  LDD2204  [3]
 LDCM0614  Fragment56 Ramos C226(0.69)  LDD2205  [3]
 LDCM0022  KB02 Ramos C226(2.70)  LDD2182  [3]
 LDCM0023  KB03 MDA-MB-231 C226(2.64)  LDD1701  [6]
 LDCM0024  KB05 RVH-421 C344(0.88)  LDD3413  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Transitional endoplasmic reticulum ATPase (VCP) AAA ATPase family P55072

The Drug(s) Related To This Target

Approved
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Azathioprine Small molecular drug DB00993

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
4 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
5 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
6 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761