Details of the Target

General Information of Target

Target ID LDTP08966
Target Name APC membrane recruitment protein 2 (AMER2)
Gene Name AMER2
Gene ID 219287
Synonyms
FAM123A; APC membrane recruitment protein 2; Amer2; Protein FAM123A
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
METSRSRGGGGAVSERGGAGASVGVCRRKAEAGAGTGTLAADMDLHCDCAAETPAAEPPS
GKINKAAFKLFKKRKSGGTMPSIFGVKNKGDGKSSGPTGLVRSRTHDGLAEVLVLESGRK
EEPRGGGDSGGGGGGRPNPGPPRAAGPGGGSLASSSVAKSHSFFSLLKKNGRSENGKGEP
VDASKAGGKQKRGLRGLFSGMRWHRKDKRAKAEAAEGRAPGGGLILPGSLTASLECVKEE
TPRAAREPEEPSQDAPRDPAGEPAGGEEVPAPADRAPARSCREAEGLAHPGDTGARGEDA
AGHRRAEPGPGEVRTAEDASRTGAVPVKTVPLVDSEGGSGRAPAAPDPASVDPPSDPSAD
RICLMFSDVTSLKSFDSLTGCGDIIADQEEEAGPSCDKHVPGPGKPALSKKNPGVVAYQG
GGEEMASPDEVDDTYLQEFWDMLSQTEEQGPEPQEGAAKVAAALETKVVPETPKDTRCVE
AAKDASSVKRRRLNRIPIEPHPKEEPKHPEKEQQEGVPNSDEGYWDSTTPGPEEDSSSSG
KKAGIPRDSYSGDALYDLYADPDGSPATLPGGKDNEETSSLSRLKPVSPGTITCPLRTPG
SLLKDSKIPISIKHLTNLPSSHPVVHQQPSRSEMPRTKIPVSKVLVRRVSNRGLAGTTIR
ATACHDSAKKL
Target Bioclass
Other
Family
Amer family
Subcellular location
Cell membrane
Function
Negative regulator of the canonical Wnt signaling pathway involved in neuroectodermal patterning. Acts by specifically binding phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2), translocating to the cell membrane and interacting with key regulators of the canonical Wnt signaling pathway, such as components of the beta-catenin destruction complex.
Uniprot ID
Q8N7J2
Ensemble ID
ENST00000357816.2
HGNC ID
HGNC:26360

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CMK SNV: p.E336D .
EFO27 SNV: p.R647C .
HL60 SNV: p.D274A .
IM95 Deletion: p.G222AfsTer3 .
MEWO SNV: p.G90E .
MFE319 Insertion: p.G85WfsTer178 .
OSRC2 SNV: p.R321W .
OZ SNV: p.M1? .
REH SNV: p.D207Y; p.V415M .
RL952 SNV: p.A457V .
SUPT1 SNV: p.A481V .
SW1271 SNV: p.C478S .
SW756 SNV: p.R195W .
SW837 SNV: p.G228V .
TKKK SNV: p.T662M .
TOV21G SNV: p.S165Ter .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C26(1.63); C594(1.79)  LDD3423  [1]
m-APA
 Probe Info  		N.A.  LDD2232  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0022  KB02 A673 C26(2.23); C594(5.78)  LDD2261  [1]
 LDCM0023  KB03 A673 C26(2.14); C594(4.88)  LDD2678  [1]
 LDCM0024  KB05 SK-ES1 C26(1.63); C594(1.79)  LDD3423  [1]

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377