Details of the Target

General Information of Target

Target ID LDTP08776
Target Name Protein THEMIS (THEMIS)
Gene Name THEMIS
Gene ID 387357
Synonyms
C6orf190; C6orf207; Protein THEMIS; Thymocyte-expressed molecule involved in selection
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MALSLEEFVHSLDLRTLPRVLEIQAGIYLEGSIYEMFGNECCFSTGEVIKITGLKVKKII
AEICEQIEGCESLQPFELPMNFPGLFKIVADKTPYLTMEEITRTIHIGPSRLGHPCFYHQ
KDIKLENLIIKQGEQIMLNSVEEIDGEIMVSCAVARNHQTHSFNLPLSQEGEFYECEDER
IYTLKEIVEWKIPKNRTRTVNLTDFSNKWDSTNPFPKDFYGTLILKPVYEIQGVMKFRKD
IIRILPSLDVEVKDITDSYDANWFLQLLSTEDLFEMTSKEFPIVTEVIEAPEGNHLPQSI
LQPGKTIVIHKKYQASRILASEIRSNFPKRHFLIPTSYKGKFKRRPREFPTAYDLEIAKS
EKEPLHVVATKAFHSPHDKLSSVSVGDQFLVHQSETTEVLCEGIKKVVNVLACEKILKKS
YEAALLPLYMEGGFVEVIHDKKQYPISELCKQFRLPFNVKVSVRDLSIEEDVLAATPGLQ
LEEDITDSYLLISDFANPTECWEIPVGRLNMTVQLVSNFSRDAEPFLVRTLVEEITEEQY
YMMRRYESSASHPPPRPPKHPSVEETKLTLLTLAEERTVDLPKSPKRHHVDITKKLHPNQ
AGLDSKVLIGSQNDLVDEEKERSNRGATAIAETFKNEKHQK
Target Bioclass
Other
Family
Themis family
Subcellular location
Cytoplasm
Function
Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk.
Uniprot ID
Q8N1K5
Ensemble ID
ENST00000368248.5
HGNC ID
HGNC:21569

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A375 SNV: p.P498R .
A3KAW SNV: p.Y489F .
CAL120 SNV: p.I49M .
CAL33 SNV: p.T578K .
FTC133 SNV: p.P555H .
HCT15 SNV: p.E414K .
HT115 SNV: p.K442N .
IGR1 SNV: p.E289K .
LN18 SNV: p.Q135Ter .
LOXIMVI SNV: p.R324K .
LS180 SNV: p.S278T .
MELJUSO SNV: p.T16A .
MEWO SNV: p.G403E .
MOLT4 SNV: p.M511T IA-alkyne    Probe Info 
NCIH2286 SNV: p.P246R .
RL SNV: p.N636K .
SKN SNV: p.R330T .
WM1552C SNV: p.P505S .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C413(3.37); C450(4.17)  LDD3397  [1]
4-Iodoacetamidophenylacetylene
 Probe Info  		C116(0.00); C176(0.00); C450(0.00); C413(0.00)  LDD0038  [2]
IA-alkyne
 Probe Info  		C413(0.00); C116(0.00); C176(0.00); C450(0.00)  LDD0036  [2]
IPIAA_L
 Probe Info  		N.A.  LDD0031  [3]
Lodoacetamide azide
 Probe Info  		C116(0.00); C176(0.00); C413(0.00); C401(0.00)  LDD0037  [2]
Compound 10
 Probe Info  		N.A.  LDD2216  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0022  KB02 T cell C450(5.46)  LDD1703  [5]
 LDCM0023  KB03 DEL C413(3.64); C81(2.47); C450(6.12)  LDD2732  [1]
 LDCM0024  KB05 PF-382 C413(3.37); C450(4.17)  LDD3397  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein arginine N-methyltransferase 6 (PRMT6) Protein arginine N-methyltransferase family Q96LA8
Tyrosine-protein kinase Lck (LCK) Tyr protein kinase family P06239
Tyrosine-protein kinase ZAP-70 (ZAP70) Tyr protein kinase family P43403
Tyrosine-protein phosphatase non-receptor type 6 (PTPN6) Protein-tyrosine phosphatase family P29350
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 (PLCG1) . P19174
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Growth factor receptor-bound protein 2 (GRB2) GRB2/sem-5/DRK family P62993

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
3 SP3-Enabled Rapid and High Coverage Chemoproteomic Identification of Cell-State-Dependent Redox-Sensitive Cysteines. Mol Cell Proteomics. 2022 Apr;21(4):100218. doi: 10.1016/j.mcpro.2022.100218. Epub 2022 Feb 25.
Mass spectrometry data entry: PXD029500 , PXD031647
4 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279
5 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.