Details of the Target

General Information of Target

Target ID	LDTP08660
Target Name	Ribonuclease SLFN12 (SLFN12)
Gene Name	SLFN12
Gene ID	55106
Synonyms	Ribonuclease SLFN12; EC 3.1.-.-; Schlafen family member 12
3D Structure	Download 2D Sequence (FASTA) Download 3D Structure (PDB) Download
Sequence	MNISVDLETNYAELVLDVGRVTLGENSRKKMKDCKLRKKQNESVSRAMCALLNSGGGVIK AEIENEDYSYTKDGIGLDLENSFSNILLFVPEYLDFMQNGNYFLIFVKSWSLNTSGLRIT TLSSNLYKRDITSAKVMNATAALEFLKDMKKTRGRLYLRPELLAKRPCVDIQEENNMKAL AGVFFDRTELDRKEKLTFTESTHVEIKNFSTEKLLQRIKEILPQYVSAFANTDGGYLFIG LNEDKEIIGFKAEMSDLDDLEREIEKSIRKMPVHHFCMEKKKINYSCKFLGVYDKGSLCG YVCALRVERFCCAVFAKEPDSWHVKDNRVMQLTRKEWIQFMVEAEPKFSSSYEEVISQIN TSLPAPHSWPLLEWQRQRHHCPGLSGRITYTPENLCRKLFLQHEGLKQLICEEMDSVRKG SLIFSRSWSVDLGLQENHKVLCDALLISQDSPPVLYTFHMVQDEEFKGYSTQTALTLKQK LAKIGGYTKKVCVMTKIFYLSPEGMTSCQYDLRSQVIYPESYYFTRRKYLLKALFKALKR LKSLRDQFSFAENLYQIIGIDCFQKNDKKMFKSCRRLT
Target Bioclass	Enzyme
Family	Schlafen family, Subgroup II subfamily
Subcellular location	Nucleus
Function	Ribonuclease which is part of an E2/17beta-estradiol-induced pro-apoptotic signaling pathway. E2 stabilizes the PDE3A/SLFN12 complex in the cytosol, promoting the dephosphorylation of SLFN12 and activating its pro-apoptotic ribosomal RNA/rRNA ribonuclease activity. This apoptotic pathway might be relevant in tissues with high concentration of E2 and be for instance involved in placenta remodeling. May play a role in cell differentiation.
Uniprot ID	Q8IYM2
Ensemble ID	ENST00000304905.10
HGNC ID	HGNC:25500

Probe(s) Labeling This Target

ABPP Probe

Click To Hide/Show 1 Probe Related to This Target

Probe name		Structure	Binding Site(Ratio)	Interaction ID	Ref
IA-alkyne Probe Info			C287(14.67)	LDD1704	[2]

Competitor(s) Related to This Target

Competitor ID	Name	Cell line	Binding Site(Ratio)	Interaction ID	Ref
LDCM0022	KB02	T cell-activated	C287(14.67)	LDD1704	[2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme

Click To Hide/Show 4 Protein(s) Interacting with This Target

Protein name	Family	Uniprot ID
Lipoprotein lipase (LPL)	Lipase family	P06858
Tyrosine--tRNA ligase, cytoplasmic (YARS1)	Class-I aminoacyl-tRNA synthetase family	P54577
Plasma kallikrein (KLKB1)	Peptidase S1 family	P03952
GTP-binding nuclear protein Ran (RAN)	Ran family	P62826

Other

Click To Hide/Show 5 Protein(s) Interacting with This Target

Protein name	Family	Uniprot ID
Cholecystokinin (CCK)	Gastrin/cholecystokinin family	P06307
Prelamin-A/C (LMNA)	Intermediate filament family	P02545
Protein kinase C and casein kinase substrate in neurons protein 1 (PACSIN1)	PACSIN family	Q9BY11
Angiotensinogen (AGT)	Serpin family	P01019
Sprouty-related, EVH1 domain-containing protein 1 (SPRED1)	.	Q7Z699

References

1	Labeling Preferences of Diazirines with Protein Biomolecules. J Am Chem Soc. 2021 May 5;143(17):6691-6700. doi: 10.1021/jacs.1c02509. Epub 2021 Apr 20. Mass spectrometry data entry: PXD025140
2	An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.