Details of the Target

General Information of Target

Target ID LDTP08516
Target Name Probable E3 ubiquitin-protein ligase HERC6 (HERC6)
Gene Name HERC6
Gene ID 55008
Synonyms
Probable E3 ubiquitin-protein ligase HERC6; EC 2.3.2.26; HECT domain and RCC1-like domain-containing protein 6; HECT-type E3 ubiquitin transferase HERC6
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MYFCWGADSRELQRRRTAGSPGAELLQAASGERHSLLLLTNHRVLSCGDNSRGQLGRRGA
QRGELPEPIQALETLIVDLVSCGKEHSLAVCHKGRVFAWGAGSEGQLGIGEFKEISFTPK
KIMTLNDIKIIQVSCGHYHSLALSKDSQVFSWGKNSHGQLGLGKEFPSQASPQRVRSLEG
IPLAQVAAGGAHSFALSLCGTSFGWGSNSAGQLALSGRNVPVQSNKPLSVGALKNLGVVY
ISCGDAHTAVLTQDGKVFTFGDNRSGQLGYSPTPEKRGPQLVERIDGLVSQIDCGSYHTL
AYVHTTGQVVSFGHGPSDTSKPTHPEALTENFDISCLISAEDFVDVQVKHIFAGTYANFV
TTHQDTSSTRAPGKTLPEISRISQSMAEKWIAVKRRSTEHEMAKSEIRMIFSSPACLTAS
FLKKRGTGETTSIDVDLEMARDTFKKLTKKEWISSMITTCLEDDLLRALPCHSPHQEALS
VFLLLPECPVMHDSKNWKNLVVPFAKAVCEMSKQSLQVLKKCWAFLQESSLNPLIQMLKA
AIISQLLHQTKTEQDHCNVKALLGMMKELHKVNKANCRLPENTFNINELSNLLNFYIDRG
RQLFRDNHLIPAETPSPVIFSDFPFIFNSLSKIKLLQADSHIKMQMSEKKAYMLMHETIL
QKKDEFPPSPRFILRVRRSRLVKDALRQLSQAEATDFCKVLVVEFINEICPESGGVSSEF
FHCMFEEMTKPEYGMFMYPEMGSCMWFPAKPKPEKKRYFLFGMLCGLSLFNLNVANLPFP
LALYKKLLDQKPSLEDLKELSPRLGKSLQEVLDDAADDIGDALCIRFSIHWDQNDVDLIP
NGISIPVDQTNKRDYVSKYIDYIFNVSVKAVYEEFQRGFYRVCEKEILRHFYPEELMTAI
IGNTDYDWKQFEQNSKYEQGYQKSHPTIQLFWKAFHKLTLDEKKKFLFFLTGRDRLHARG
IQKMEIVFRCPETFSERDHPTSITCHNILSLPKYSTMERMEEALQVAINNNRGFVSPMLT
QS
Target Bioclass
Enzyme
Subcellular location
Cytoplasm, cytosol
Function E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.
Uniprot ID
Q8IVU3
Ensemble ID
ENST00000264346.12
HGNC ID
HGNC:26072

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
22RV1 SNV: p.G564Ter .
769P Deletion: p.L328_T329del .
AGS Substitution: p.P1017S .
CHL1 SNV: p.E874K .
DU145 SNV: p.G211W .
HCT15 SNV: p.M565V .
JVM3 SNV: p.K786I .
KELLY SNV: p.G53A .
MDAMB453 SNV: p.D814H .
NB1 SNV: p.I928R .
NCIH446 SNV: p.G163E .
OV90 SNV: p.W99S .
REH SNV: p.A540P .
SW620 SNV: p.G59S .
TKKK Insertion: p.L764dup .
TOV21G SNV: p.H86Q .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K574(5.00)  LDD0277  [1]
DBIA
 Probe Info  		C47(2.13)  LDD3310  [2]
IA-alkyne
 Probe Info  		C557(20.00)  LDD1705  [3]
NAIA_4
 Probe Info  		N.A.  LDD2226  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0022  KB02 BRX211 C47(1.51)  LDD2268  [2]
 LDCM0023  KB03 BRX211 C47(1.65)  LDD2685  [2]
 LDCM0024  KB05 COLO792 C47(2.13)  LDD3310  [2]

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
4 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264