Details of the Target

General Information of Target

Target ID LDTP08372
Target Name Centrosomal protein of 57 kDa (CEP57)
Gene Name CEP57
Gene ID 9702
Synonyms
KIAA0092; TSP57; Centrosomal protein of 57 kDa; Cep57; FGF2-interacting protein; Testis-specific protein 57; Translokin
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAAASVSAASGSHLSNSFAEPSRSNGSMVRHSSSPYVVYPSDKPFLNSDLRRSPSKPTLA
YPESNSRAIFSALKNLQDKIRRLELERIQAEESVKTLSRETIEYKKVLDEQIQERENSKN
EESKHNQELTSQLLAAENKCNLLEKQLEYMRNMIKHAEMERTSVLEKQVSLERERQHDQT
HVQSQLEKLDLLEQEYNKLTTMQALAEKKMQELEAKLHEEEQERKRMQAKAAELQTGLET
NRLIFEDKATPCVPNARRIKKKKSKPPEKKSSRNYFGAQPHYRLCLGDMPFVAGKSTSPS
HAVVANVQLVLHLMKQHSKALCNDRVINSIPLAKQVSSRGGKSKKLSVTPPSSNGINEEL
SEVLQTLQDEFGQMSFDHQQLAKLIQESPTVELKDKLECELEALVGRMEAKANQITKVRK
YQAQLEKQKLEKQKKELKATKKTLDEERNSSSRSGITGTTNKKDFMKLRPGEKRRKNLQL
LKDMQSIQNSLQSSSLCWDY
Target Bioclass
Other
Family
Translokin family
Subcellular location
Nucleus
Function
Centrosomal protein which may be required for microtubule attachment to centrosomes. May act by forming ring-like structures around microtubules. Mediates nuclear translocation and mitogenic activity of the internalized growth factor FGF2, but that of FGF1.
Uniprot ID
Q86XR8
Ensemble ID
ENST00000325486.9
HGNC ID
HGNC:30794

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
22RV1 Deletion: p.K262RfsTer31 .
TOV21G Deletion: p.K262RfsTer31 .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 7 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C252(12.18)  LDD0209  [1]
IA-alkyne
 Probe Info  		N.A.  LDD0165  [2]
NAIA_4
 Probe Info  		N.A.  LDD2226  [3]
Compound 10
 Probe Info  		N.A.  LDD2216  [4]
Compound 11
 Probe Info  		N.A.  LDD2213  [4]
IPM
 Probe Info  		N.A.  LDD0147  [5]
TFBX
 Probe Info  		C285(0.00); C252(0.00)  LDD0148  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0226  AC11 HEK-293T C399(1.09)  LDD1509  [6]
 LDCM0278  AC19 HEK-293T C399(1.17)  LDD1517  [6]
 LDCM0287  AC27 HEK-293T C399(1.03)  LDD1526  [6]
 LDCM0290  AC3 HEK-293T C399(1.05)  LDD1529  [6]
 LDCM0296  AC35 HEK-293T C399(1.05)  LDD1535  [6]
 LDCM0305  AC43 HEK-293T C399(1.00)  LDD1544  [6]
 LDCM0314  AC51 HEK-293T C399(1.06)  LDD1553  [6]
 LDCM0322  AC59 HEK-293T C399(1.04)  LDD1561  [6]
 LDCM0367  CL1 HEK-293T C399(0.96)  LDD1571  [6]
 LDCM0370  CL101 HEK-293T C399(1.01)  LDD1574  [6]
 LDCM0372  CL103 HEK-293T C399(0.94)  LDD1576  [6]
 LDCM0374  CL105 HEK-293T C399(0.95)  LDD1578  [6]
 LDCM0376  CL107 HEK-293T C399(0.90)  LDD1580  [6]
 LDCM0378  CL109 HEK-293T C399(0.97)  LDD1582  [6]
 LDCM0381  CL111 HEK-293T C399(0.90)  LDD1585  [6]
 LDCM0383  CL113 HEK-293T C399(1.00)  LDD1587  [6]
 LDCM0385  CL115 HEK-293T C399(1.08)  LDD1589  [6]
 LDCM0387  CL117 HEK-293T C399(1.01)  LDD1591  [6]
 LDCM0389  CL119 HEK-293T C399(1.02)  LDD1593  [6]
 LDCM0392  CL121 HEK-293T C399(0.90)  LDD1596  [6]
 LDCM0394  CL123 HEK-293T C399(0.91)  LDD1598  [6]
 LDCM0396  CL125 HEK-293T C399(1.04)  LDD1600  [6]
 LDCM0398  CL127 HEK-293T C399(1.07)  LDD1602  [6]
 LDCM0400  CL13 HEK-293T C399(1.01)  LDD1604  [6]
 LDCM0402  CL15 HEK-293T C399(0.86)  LDD1606  [6]
 LDCM0406  CL19 HEK-293T C399(0.89)  LDD1610  [6]
 LDCM0413  CL25 HEK-293T C399(0.94)  LDD1617  [6]
 LDCM0415  CL27 HEK-293T C399(0.97)  LDD1619  [6]
 LDCM0418  CL3 HEK-293T C399(0.92)  LDD1622  [6]
 LDCM0420  CL31 HEK-293T C399(0.97)  LDD1624  [6]
 LDCM0426  CL37 HEK-293T C399(0.93)  LDD1630  [6]
 LDCM0428  CL39 HEK-293T C399(0.91)  LDD1632  [6]
 LDCM0433  CL43 HEK-293T C399(1.06)  LDD1637  [6]
 LDCM0439  CL49 HEK-293T C399(0.95)  LDD1643  [6]
 LDCM0446  CL55 HEK-293T C399(1.05)  LDD1649  [6]
 LDCM0453  CL61 HEK-293T C399(0.98)  LDD1656  [6]
 LDCM0455  CL63 HEK-293T C399(1.01)  LDD1658  [6]
 LDCM0459  CL67 HEK-293T C399(0.95)  LDD1662  [6]
 LDCM0462  CL7 HEK-293T C399(1.11)  LDD1665  [6]
 LDCM0466  CL73 HEK-293T C399(1.02)  LDD1669  [6]
 LDCM0472  CL79 HEK-293T C399(1.04)  LDD1675  [6]
 LDCM0479  CL85 HEK-293T C399(1.02)  LDD1682  [6]
 LDCM0481  CL87 HEK-293T C399(1.05)  LDD1684  [6]
 LDCM0486  CL91 HEK-293T C399(1.13)  LDD1689  [6]
 LDCM0492  CL97 HEK-293T C399(0.97)  LDD1695  [6]
 LDCM0494  CL99 HEK-293T C399(0.90)  LDD1697  [6]
 LDCM0495  E2913 HEK-293T C399(0.96)  LDD1698  [6]
 LDCM0468  Fragment33 HEK-293T C399(1.02)  LDD1671  [6]
 LDCM0022  KB02 T cell-activated C285(5.25)  LDD1704  [7]
 LDCM0023  KB03 Jurkat C252(12.18)  LDD0209  [1]
 LDCM0024  KB05 NCI-H1975 C252(2.08)  LDD3352  [8]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Lysine-specific histone demethylase 1A (KDM1A) Flavin monoamine oxidase family O60341
Serine/threonine-protein kinase N1 (PKN1) AGC Ser/Thr protein kinase family Q16512
Fibroblast growth factor receptor 3 (FGFR3) Tyr protein kinase family P22607
GTPase HRas (HRAS) Ras family P01112
F-box/LRR-repeat protein 3 (FBXL3) . Q9UKT7
RalA-binding protein 1 (RALBP1) . Q15311
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Melanoma inhibitory activity protein 2 (MIA2) MIA/OTOR family Q96PC5
Transcription factor
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
SAM pointed domain-containing Ets transcription factor (SPDEF) ETS family O95238
Myc proto-oncogene protein (MYC) . P01106
Other
Click To Hide/Show 29 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Apoptosis-stimulating of p53 protein 1 (PPP1R13B) ASPP family Q96KQ4
ATP synthase mitochondrial F1 complex assembly factor 2 (ATPAF2) ATP12 family Q8N5M1
Testis-specific gene 10 protein (TSGA10) CEP135/TSGA10 family Q9BZW7
Centrosomal protein of 63 kDa (CEP63) CEP63 family Q96MT8
Deuterosome assembly protein 1 (DEUP1) CEP63 family Q05D60
Keratin, type I cuticular Ha1 (KRT31) Intermediate filament family Q15323
Keratin, type I cytoskeletal 15 (KRT15) Intermediate filament family P19012
Keratin, type I cytoskeletal 40 (KRT40) Intermediate filament family Q6A162
Keratin, type II cuticular Hb3 (KRT83) Intermediate filament family P78385
Keratin, type II cuticular Hb6 (KRT86) Intermediate filament family O43790
Keratin, type II cytoskeletal 4 (KRT4) Intermediate filament family P19013
Keratin, type II cytoskeletal 75 (KRT75) Intermediate filament family O95678
Keratin, type II cytoskeletal 8 (KRT8) Intermediate filament family P05787
Kinesin light chain 4 (KLC4) Kinesin light chain family Q9NSK0
Protein Mis18-beta (OIP5) Mis18 family O43482
Testis-specific Y-encoded-like protein 4 (TSPYL4) Nucleosome assembly protein (NAP) family Q9UJ04
Rab GTPase-binding effector protein 1 (RABEP1) Rabaptin family Q15276
SH3 domain-binding protein 5-like (SH3BP5L) SH3BP5 family Q7L8J4
Alpha-taxilin (TXLNA) Taxilin family P40222
Beta-taxilin (TXLNB) Taxilin family Q8N3L3
Tuftelin-interacting protein 11 (TFIP11) TFP11/STIP family Q9UBB9
Alanine and arginine-rich domain-containing protein (AARD) . Q4LEZ3
Coiled-coil domain-containing protein 185 (CCDC185) . Q8N715
GRIP and coiled-coil domain-containing protein 1 (GCC1) . Q96CN9
Heat shock factor 2-binding protein (HSF2BP) . O75031
Melanoma-associated antigen 2 (MAGEA2; MAGEA2B) . P43356
Ras association domain-containing protein 10 (RASSF10) . A6NK89
Sprouty-related, EVH1 domain-containing protein 1 (SPRED1) . Q7Z699
Testis-expressed protein 12 (TEX12) . Q9BXU0

References

1 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
2 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
3 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
4 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279
5 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
6 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
7 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
8 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840