Details of the Target

General Information of Target

Target ID LDTP08342
Target Name Src kinase-associated phosphoprotein 1 (SKAP1)
Gene Name SKAP1
Gene ID 8631
Synonyms
SCAP1; SKAP55; Src kinase-associated phosphoprotein 1; Src family-associated phosphoprotein 1; Src kinase-associated phosphoprotein of 55 kDa; SKAP-55; pp55
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MQAAALPEEIRWLLEDAEEFLAEGLRNENLSAVARDHRDHILRGFQQIKARYYWDFQPQG
GDIGQDSSDDNHSGTLGLSLTSDAPFLSDYQDEGMEDIVKGAQELDNVIKQGYLEKKSKD
HSFFGSEWQKRWCVVSRGLFYYYANEKSKQPKGTFLIKGYGVRMAPHLRRDSKKESCFEL
TSQDRRSYEFTATSPAEARDWVDQISFLLKDLSSLTIPYEEDEEEEEKEETYDDIDGFDS
PSCGSQCRPTILPGSVGIKEPTEEKEEEDIYEVLPDEEHDLEEDESGTRRKGVDYASYYQ
GLWDCHGDQPDELSFQRGDLIRILSKEYNMYGWWVGELNSLVGIVPKEYLTTAFEVEER
Target Bioclass
Other
Family
SKAP family
Subcellular location
Cytoplasm
Function
Positively regulates T-cell receptor signaling by enhancing the MAP kinase pathway. Required for optimal conjugation between T-cells and antigen-presenting cells by promoting the clustering of integrin ITGAL on the surface of T-cells. May be involved in high affinity immunoglobulin epsilon receptor signaling in mast cells.
Uniprot ID
Q86WV1
Ensemble ID
ENST00000336915.11
HGNC ID
HGNC:15605

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
BICR22 SNV: p.K117T .
DU145 Substitution: p.Q204L .
IM95 SNV: p.C133R .
LNCaP clone FGC Insertion: p.D239Ter .
NCIH2286 Substitution: p.E327R .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C177(3.81)  LDD3397  [1]
Acrolein
 Probe Info  		N.A.  LDD0233  [2]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [3]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0022  KB02 DEL C177(1.57)  LDD2315  [1]
 LDCM0023  KB03 Jurkat C177(2.48)  LDD2826  [1]
 LDCM0024  KB05 PF-382 C177(3.81)  LDD3397  [1]
 LDCM0109  NEM HeLa N.A.  LDD0233  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Caspase-7 (CASP7) Peptidase C14A family P55210
Protein disulfide-isomerase (P4HB) Protein disulfide isomerase family P07237
Other
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Bladder cancer-associated protein (BLCAP) BLCAP family P62952
Early estrogen-induced gene 1 protein (EEIG1) EEIG family Q5T9C2
FYN-binding protein 1 (FYB1) . O15117
PML-RARA-regulated adapter molecule 1 (PRAM1) . Q96QH2
SH2 domain-containing protein 4A (SH2D4A) . Q9H788

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
3 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853