Details of the Target

General Information of Target

Target ID LDTP08308
Target Name Testis-specific Y-encoded-like protein 5 (TSPYL5)
Gene Name TSPYL5
Gene ID 85453
Synonyms
KIAA1750; Testis-specific Y-encoded-like protein 5; TSPY-like protein 5
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MSGRSRGRKSSRAKNRGKGRAKARVRPAPDDAPRDPDPSQYQSLGEDTQAAQVQAGAGWG
GLEAAASAQLLRLGEEAACRLPLDCGLALRARAAGDHGQAAARPGPGKAASLSERLAADT
VFVGTAGTVGRPKNAPRVGNRRGPAGKKAPETCSTAGRGPQVIAGGRQKKGAAGENTSVS
AGEEKKEERDAGSGPPATEGSMDTLENVQLKLENMNAQADRAYLRLSRKFGQLRLQHLER
RNHLIQNIPGFWGQAFQNHPQLASFLNSQEKEVLSYLNSLEVEELGLARLGYKIKFYFDR
NPYFQNKVLIKEYGCGPSGQVVSRSTPIQWLPGHDLQSLSQGNPENNRSFFGWFSNHSSI
ESDKIVEIINEELWPNPLQFYLLSEGARVEKGKEKEGRQGPGKQPMETTQPGVSQSN
Target Bioclass
Other
Family
Nucleosome assembly protein (NAP) family
Function
Involved in modulation of cell growth and cellular response to gamma radiation probably via regulation of the Akt signaling pathway. Involved in regulation of p53/TP53. Suppresses p53/TP53 protein levels and promotes its ubiquitination; the function is dependent on USP7 and independent on MDM2. Proposed to displace p53/TP53 from interaction with USP7.
Uniprot ID
Q86VY4
Ensemble ID
ENST00000322128.5
HGNC ID
HGNC:29367

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 11 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0402  [1]
BTD
 Probe Info  		C153(0.97)  LDD2093  [2]
DBIA
 Probe Info  		C79(22.48); C85(8.99); C315(6.34)  LDD0209  [3]
IPM
 Probe Info  		C153(1.41)  LDD1701  [2]
4-Iodoacetamidophenylacetylene
 Probe Info  		C315(0.00); C85(0.00); C153(0.00)  LDD0038  [4]
IA-alkyne
 Probe Info  		C315(0.00); C79(0.00); C153(0.00)  LDD0036  [4]
Lodoacetamide azide
 Probe Info  		C315(0.00); C79(0.00); C85(0.00); C153(0.00)  LDD0037  [4]
WYneN
 Probe Info  		N.A.  LDD0021  [5]
Compound 10
 Probe Info  		C153(0.00); C315(0.00); C79(0.00); C85(0.00)  LDD2216  [6]
Compound 11
 Probe Info  		C315(0.00); C85(0.00)  LDD2213  [6]
NAIA_5
 Probe Info  		C153(0.00); C85(0.00); C315(0.00)  LDD2223  [7]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0156  Aniline NCI-H1299 11.93  LDD0403  [1]
 LDCM0632  CL-Sc Hep-G2 C85(1.86); C315(0.91); C153(0.90); C153(0.59)  LDD2227  [7]
 LDCM0634  CY-0357 Hep-G2 C79(1.88); C315(0.86); C85(0.53)  LDD2228  [7]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C153(1.05)  LDD1702  [2]
 LDCM0022  KB02 769-P C153(1.14)  LDD2246  [8]
 LDCM0023  KB03 Jurkat C79(22.48); C85(8.99); C315(6.34)  LDD0209  [3]
 LDCM0024  KB05 COLO792 C315(1.61); C85(2.03)  LDD3310  [8]
 LDCM0500  Nucleophilic fragment 13a MDA-MB-231 C153(0.97)  LDD2093  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Ubiquitin carboxyl-terminal hydrolase 7 (USP7) Peptidase C19 family Q93009

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
3 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
4 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
5 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
6 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279
7 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
8 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840