Details of the Target

General Information of Target

Target ID LDTP08221
Target Name E3 ubiquitin-protein ligase MARCHF3 (MARCHF3)
Gene Name MARCHF3
Gene ID 115123
Synonyms
MARCH3; RNF173; E3 ubiquitin-protein ligase MARCHF3; EC 2.3.2.27; Membrane-associated RING finger protein 3; Membrane-associated RING-CH protein III; MARCH-III; RING finger protein 173; RING-type E3 ubiquitin transferase MARCHF3
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MTTSRCSHLPEVLPDCTSSAAPVVKTVEDCGSLVNGQPQYVMQVSAKDGQLLSTVVRTLA
TQSPFNDRPMCRICHEGSSQEDLLSPCECTGTLGTIHRSCLEHWLSSSNTSYCELCHFRF
AVERKPRPLVEWLRNPGPQHEKRTLFGDMVCFLFITPLATISGWLCLRGAVDHLHFSSRL
EAVGLIALTVALFTIYLFWTLVSFRYHCRLYNEWRRTNQRVILLIPKSVNVPSNQPSLLG
LHSVKRNSKETVV
Target Bioclass
Enzyme
Subcellular location
Cytoplasmic vesicle membrane
Function
E3 ubiquitin-protein ligase which may be involved in endosomal trafficking. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
Uniprot ID
Q86UD3
Ensemble ID
ENST00000308660.6
HGNC ID
HGNC:28728

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A431 SNV: p.L145V .
HT115 SNV: p.T217A .
KU1919 SNV: p.Y112C .
MEWO SNV: p.D148N .
YSCCC SNV: p.Y112C .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C16(1.29); C6(1.29)  LDD0078  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0020  ARS-1620 HCC44 C16(1.29); C6(1.29)  LDD0078  [1]

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.