Details of the Target

General Information of Target

Target ID LDTP08075
Target Name Prolyl 4-hydroxylase subunit alpha-3 (P4HA3)
Gene Name P4HA3
Gene ID 283208
Synonyms
Prolyl 4-hydroxylase subunit alpha-3; 4-PH alpha-3; EC 1.14.11.2; Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-3
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MGPGARLAALLAVLALGTGDPERAAARGDTFSALTSVARALAPERRLLGLLRRYLRGEEA
RLRDLTRFYDKVLSLHEDSTTPVANPLLAFTLIKRLQSDWRNVVHSLEASENIRALKDGY
EKVEQDLPAFEDLEGAARALMRLQDVYMLNVKGLARGVFQRVTGSAITDLYSPKRLFSLT
GDDCFQVGKVAYDMGDYYHAIPWLEEAVSLFRGSYGEWKTEDEASLEDALDHLAFAYFRA
GNVSCALSLSREFLLYSPDNKRMARNVLKYERLLAESPNHVVAEAVIQRPNIPHLQTRDT
YEGLCQTLGSQPTLYQIPSLYCSYETNSNAYLLLQPIRKEVIHLEPYIALYHDFVSDSEA
QKIRELAEPWLQRSVVASGEKQLQVEYRISKSAWLKDTVDPKLVTLNHRIAALTGLDVRP
PYAEYLQVVNYGIGGHYEPHFDHATSPSSPLYRMKSGNRVATFMIYLSSVEAGGATAFIY
ANLSVPVVRNAALFWWNLHRSGEGDSDTLHAGCPVLVGDKWVANKWIHEYGQEFRRPCSS
SPED
Target Bioclass
Enzyme
Family
P4HA family
Subcellular location
Endoplasmic reticulum lumen
Function Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.
Uniprot ID
Q7Z4N8
Ensemble ID
ENST00000331597.9
HGNC ID
HGNC:30135

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CORL23 SNV: p.S248R .
HS936T SNV: p.L247I .
HT115 SNV: p.Q316H; p.A411V .
MCC13 SNV: p.Q288Ter .
NCIH82 SNV: p.A492T .
SHSY5Y SNV: p.G213R .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
Acrolein
 Probe Info  		N.A.  LDD0222  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 7 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Creatine kinase S-type, mitochondrial (CKMT2) ATP:guanido phosphotransferase family P17540
Acyl-coenzyme A thioesterase 8 (ACOT8) C/M/P thioester hydrolase family O14734
Inactive glycosyltransferase 25 family member 3 (CERCAM) Glycosyltransferase 25 family Q5T4B2
Mitochondrial peptide methionine sulfoxide reductase (MSRA) MsrA Met sulfoxide reductase family Q9UJ68
ATP-dependent Clp protease proteolytic subunit, mitochondrial (CLPP) Peptidase S14 family Q16740
Proteasome subunit beta type-4 (PSMB4) Peptidase T1B family P28070
Endoribonuclease ZC3H12A (ZC3H12A) ZC3H12 family Q5D1E8
Transcription factor
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Homeobox protein prophet of Pit-1 (PROP1) Paired homeobox family O75360
Homeobox protein MOX-2 (MEOX2) . P50222
GPCR
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Alpha-2C adrenergic receptor (ADRA2C) G-protein coupled receptor 1 family P18825
Other
Click To Hide/Show 27 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
B9 domain-containing protein 2 (B9D2) B9D family Q9BPU9
Biogenesis of lysosome-related organelles complex 1 subunit 2 (BLOC1S2) BLOC1S2 family Q6QNY1
Cancer/testis antigen 1 (CTAG1A; CTAG1B) CTAG/PCC1 family P78358
Beta-catenin-interacting protein 1 (CTNNBIP1) CTNNBIP1 family Q9NSA3
Dysbindin (DTNBP1) Dysbindin family Q96EV8
Protein FAM136A (FAM136A) FAM136 family Q96C01
Keratin-associated protein 19-5 (KRTAP19-5) KRTAP type 19 family Q3LI72
Keratin-associated protein 6-1 (KRTAP6-1) KRTAP type 6 family Q3LI64
Keratin-associated protein 8-1 (KRTAP8-1) KRTAP type 8 family Q8IUC2
Protein Mis18-beta (OIP5) Mis18 family O43482
NUT family member 1 (NUTM1) NUT family Q86Y26
Stathmin-3 (STMN3) Stathmin family Q9NZ72
Vacuolar protein sorting-associated protein 37C (VPS37C) VPS37 family A5D8V6
AP-4 complex accessory subunit RUSC1 (RUSC1) . Q9BVN2
BAG family molecular chaperone regulator 4 (BAG4) . O95429
Cancer/testis antigen 55 (CT55) . Q8WUE5
Complement C1q tumor necrosis factor-related protein 2 (C1QTNF2) . Q9BXJ5
FIGNL1-interacting regulator of recombination and mitosis (FIRRM) . Q9NSG2
G-protein-signaling modulator 3 (GPSM3) . Q9Y4H4
Hepatocyte growth factor-regulated tyrosine kinase substrate (HGS) . O14964
LRP2-binding protein (LRP2BP) . Q9P2M1
Melanoma-associated antigen 2 (MAGEA2; MAGEA2B) . P43356
Melanoma-associated antigen C2 (MAGEC2) . Q9UBF1
Mitochondrial coiled-coil domain protein 1 (MCCD1) . P59942
Nicolin-1 (NICN1) . Q9BSH3
SERTA domain-containing protein 3 (SERTAD3) . Q9UJW9
WD repeat-containing protein 62 (WDR62) . O43379

References

1 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.