Details of the Target

General Information of Target

Target ID LDTP08056
Target Name E3 ubiquitin-protein ligase RNF144B (RNF144B)
Gene Name RNF144B
Gene ID 255488
Synonyms
IBRDC2; P53RFP; E3 ubiquitin-protein ligase RNF144B; EC 2.3.2.31; IBR domain-containing protein 2; RING finger protein 144B; p53-inducible RING finger protein
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MGSAGRLHYLAMTAENPTPGDLAPAPLITCKLCLCEQSLDKMTTLQECQCIFCTACLKQY
MQLAIREGCGSPITCPDMVCLNHGTLQEAEIACLVPVDQFQLYQRLKFEREVHLDPYRTW
CPVADCQTVCPVASSDPGQPVLVECPSCHLKFCSCCKDAWHAEVSCRDSQPIVLPTEHRA
LFGTDAEAPIKQCPVCRVYIERNEGCAQMMCKNCKHTFCWYCLQNLDNDIFLRHYDKGPC
RNKLGHSRASVMWNRTQVVGILVGLGIIALVTSPLLLLASPCIICCVCKSCRGKKKKHDP
STT
Target Bioclass
Enzyme
Family
RBR family, RNF144 subfamily
Subcellular location
Mitochondrion membrane
Function
E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism. However, its overexpression also produces a decrease of the ubiquitin-dependent stability of BAX, a pro-apoptotic protein, ultimately leading to protection of cell death; But, it is not an anti-apoptotic protein per se.
Uniprot ID
Q7Z419
Ensemble ID
ENST00000259939.4
HGNC ID
HGNC:21578

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
KMCH1 SNV: p.Y117Ter .
LS180 SNV: p.R202H .
MCC26 SNV: p.D77N .
MOLT4 SNV: p.P239S .
TCCSUP SNV: p.P24A .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C77(2.25)  LDD3379  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0022  KB02 22RV1 C77(1.72)  LDD2243  [1]
 LDCM0023  KB03 22RV1 C77(2.26)  LDD2660  [1]
 LDCM0024  KB05 OV56 C77(2.25)  LDD3379  [1]

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840