Details of the Target

General Information of Target

Target ID LDTP07471
Target Name PWWP domain-containing protein 2B (PWWP2B)
Gene Name PWWP2B
Gene ID 170394
Synonyms
PWWP2; PWWP domain-containing protein 2B
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MEPRAGCRLPVRVEQVVNGALVVTVSCGERSFAGILLDCTKKSGLFGLPPLAPLPQVDES
PVNDSHGRAPEEGDAEVMQLGSSSPPPARGVQPPETTRPEPPPPLVPPLPAGSLPPYPPY
FEGAPFPHPLWLRDTYKLWVPQPPPRTIKRTRRRLSRNRDPGRLILSTIRLRPRQVLCEK
CKSTLSPPEASPGPPAAPRARRRLGSGPDRELRKPEEPENGEPTAAATARRSKRERREED
RAPAEQVPRSPVIKISYSTPQGKGEVVKIPSRVHGSLEPFRPQQAPQDDGSQDPEVLDRE
SRDRPSCAPSASIPKLKLTRPVPAGADLPPPKIRLKPHRLGDSEHEPVYRAELVGELNGY
LRDSSPAPCADGPAGGLADLSSGSSGEDDDFKSCPQGPQGREGLAFLVSCPEGRADCASE
SACSSDSLDEARSSGSEGTPADTGDLSPGHGASAPSVSREARQTVPPLTVRLHTQSVSEC
ITEDGRTVAVGDIVWGKIHGFPWWPARVLDISLGQKEDGEPSWREAKVSWFGSPTTSFLS
ISKLSPFSEFFKLRFNRKKKGMYRKAITEAANAARHVAPEIRELLTQFET
Target Bioclass
Enzyme
Function
Chromatin-binding protein that acts as an adapter between distinct nucleosome components (H3K36me3 or H2A.Z) and chromatin-modifying complexes, contributing to the regulation of the levels of histone acetylation at actively transcribed genes. Competes with CHD4 and MBD3 for interaction with MTA1 to form a NuRD subcomplex, preventing the formation of full NuRD complex (containing CHD4 and MBD3), leading to recruitment of HDACs to gene promoters resulting in turn in the deacetylation of nearby H3K27 and H2A.Z. Plays a role in facilitating transcriptional elongation through regulation of histone acetylation. Negatively regulates brown adipocyte thermogenesis by interacting with and stabilizing HDAC1 at the UCP1 gene promoter, thereby promoting histone deacetylation at the promoter leading to the repression of UCP1 expression.
Uniprot ID
Q6NUJ5
Ensemble ID
ENST00000305233.6
HGNC ID
HGNC:25150

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A3KAW SNV: p.Ter591QextTer4 .
A498 SNV: p.P305T .
CCK81 SNV: p.Q175R .
EFO21 SNV: p.R524Ter .
HEC1B SNV: p.A570T .
IGR1 SNV: p.P119S .
IPC298 SNV: p.I165N .
Ishikawa (Heraklio) 02 ER Insertion: p.E95RfsTer85 .
LOXIMVI SNV: p.Y349H .
LS180 SNV: p.S183N .
MCC13 SNV: p.S256F .
MOLT4 Insertion: p.R414EfsTer7 .
OV56 SNV: p.G264R DBIA    Probe Info 
P31FUJ SNV: p.E211D .
SNU478 SNV: p.S453L DBIA    Probe Info 
SW756 SNV: p.E412D .
UMUC3 SNV: p.V17I .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C410(1.40); C394(1.77)  LDD3310  [1]
NAIA_4
 Probe Info  		N.A.  LDD2226  [2]
NAIA_5
 Probe Info  		C307(0.00); C480(0.00); C39(0.00)  LDD2224  [2]
IPM
 Probe Info  		N.A.  LDD0147  [3]
TFBX
 Probe Info  		N.A.  LDD0148  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0632  CL-Sc Hep-G2 C39(3.00); C39(0.77)  LDD2227  [2]
 LDCM0022  KB02 A-375 C394(1.56)  LDD2255  [1]
 LDCM0023  KB03 A2058 C394(1.27)  LDD2670  [1]
 LDCM0024  KB05 COLO792 C410(1.40); C394(1.77)  LDD3310  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Proteasome subunit alpha type-3 (PSMA3) Peptidase T1A family P25788
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Golgin subfamily A member 6-like protein 9 (GOLGA6L9) GOLGA6 family A6NEM1

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
3 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255