Details of the Target

General Information of Target

Target ID LDTP07436
Target Name Pyrin and HIN domain-containing protein 1 (PYHIN1)
Gene Name PYHIN1
Gene ID 149628
Synonyms
IFIX; Pyrin and HIN domain-containing protein 1; Interferon-inducible protein X
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MANNYKKIVLLKGLEVINDYHFRIVKSLLSNDLKLNPKMKEEYDKIQIADLMEEKFPGDA
GLGKLIEFFKEIPTLGDLAETLKREKLKVANKIESIPVKGIIPSKKTKQKEVYPATPACT
PSNRLTAKGAEETLGPQKRKKPSEEETGTKRSKMSKEQTRPSCSAGASTSTAMGRSPPPQ
TSSSAPPNTSSTESLKPLANRHATASKNIFREDPIIAMVLNATKVFKYESSENEQRRMFH
ATVATQTQFFHVKVLNINLKRKFIKKRIIIISNYSKRNSLLEVNEASSVSEAGPDQTFEV
PKDIIRRAKKIPKINILHKQTSGYIVYGLFMLHTKIVNRKTTIYEIQDKTGSMAVVGKGE
CHNIPCEKGDKLRLFCFRLRKRENMSKLMSEMHSFIQIQKNTNQRSHDSRSMALPQEQSQ
HPKPSEASTTLPESHLKTPQMPPTTPSSSSFTKKDETHPGAQSSPANFRITSPTVAPPLS
SDTSTNRHPAVP
Target Bioclass
Transcription factor
Family
HIN-200 family
Subcellular location
Nucleus; Nucleus, nucleoplasm
Function
Major mediator of the tumor suppressor activity of IFN in breast cancer cells. Promotes ubiquitination and subsequent degradation of MDM2, which leads to p53/TP53 stabilization. Promotes ubiquitination and subsequent degradation of HDAC1, which in turn enhances maspin expression, and impairs invasive activity of cancer cells.
Uniprot ID
Q6K0P9
Ensemble ID
ENST00000368135.4
HGNC ID
HGNC:28894

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CCK81 SNV: p.Q397R .
COLO792 SNV: p.P179L .
DU145 SNV: p.S464Ter .
Ishikawa (Heraklio) 02 ER SNV: p.M218T .
JHH7 SNV: p.E94Ter .
JURKAT Insertion: p.P142TfsTer3 .
MCC26 SNV: p.K371Ter .
MDAMB436 SNV: p.C376R .
NCIH1155 SNV: p.K349E .
NCIH196 SNV: p.S406I .
SKMEL5 SNV: p.E67K .
WM793 SNV: p.I216L .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C163(1.87)  LDD3372  [1]
Acrolein
 Probe Info  		N.A.  LDD0221  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [2]
 LDCM0107  IAA HeLa N.A.  LDD0221  [2]
 LDCM0022  KB02 HG-3 C163(2.56); C119(1.93)  LDD2355  [1]
 LDCM0023  KB03 HG-3 C163(2.68); C119(3.48)  LDD2772  [1]
 LDCM0024  KB05 OCI-Ly3 C163(1.87)  LDD3372  [1]
 LDCM0109  NEM HeLa N.A.  LDD0225  [2]

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.