Details of the Target

General Information of Target

Target ID LDTP07421
Target Name Centriole, cilia and spindle-associated protein (CCSAP)
Gene Name CCSAP
Gene ID 126731
Synonyms
C1orf96; CSAP; Centriole, cilia and spindle-associated protein
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSPGSGVKSEYMKRYQEPRWEEYGPCYRELLHYRLGRRLLEQAHAPWLWDDWGPAGSSED
SASSESSGAGGPAPRCAPPSPPPPVEPATQEEAERRARGAPEEQDAEAGDAEAEDAEDAA
LPALPVKDVEDKPEQQTRTRETDKSPTSTEPRQQPSALFARGNRKAVKSPQRSSSKIKEN
KHPFALYGWGEKQTDTGSQKTHNVCASAPVHEIHESALRAKNRRQVEKRKLVAQRQRAHS
VDVEKNRKMKASSSENPWMTEYMRCYSARA
Target Bioclass
Other
Family
CCSAP family
Subcellular location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole
Function
Plays a role in microtubule (MT) stabilization and this stabilization involves the maintenance of NUMA1 at the spindle poles. Colocalizes with polyglutamylated MTs to promote MT stabilization and regulate bipolar spindle formation in mitosis. Binding of CCSAP to centrosomes and the spindle around centrosomes during mitosis inhibits MT depolymerization, thereby stabilizing the mitotic spindle. May play a role in embryonic development. May be required for proper cilia beating.
Uniprot ID
Q6IQ19
Ensemble ID
ENST00000284617.7
HGNC ID
HGNC:29578

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
KP4 SNV: p.H182N .
LS180 SNV: p.Y33D .
MOLT4 SNV: p.V7G .
SNU449 SNV: p.E22D .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IPM
 Probe Info  		N.A.  LDD2156  [1]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [2]

References

1 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
2 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004