Details of the Target

General Information of Target

Target ID LDTP07307
Target Name Metalloreductase STEAP4 (STEAP4)
Gene Name STEAP4
Gene ID 79689
Synonyms
STAMP2; TNFAIP9; Metalloreductase STEAP4; EC 1.16.1.-; Six-transmembrane epithelial antigen of prostate 4; SixTransMembrane protein of prostate 2; Tumor necrosis factor, alpha-induced protein 9
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MEKTCIDALPLTMNSSEKQETVCIFGTGDFGRSLGLKMLQCGYSVVFGSRNPQKTTLLPS
GAEVLSYSEAAKKSGIIIIAIHREHYDFLTELTEVLNGKILVDISNNLKINQYPESNAEY
LAHLVPGAHVVKAFNTISAWALQSGALDASRQVFVCGNDSKAKQRVMDIVRNLGLTPMDQ
GSLMAAKEIEKYPLQLFPMWRFPFYLSAVLCVFLFFYCVIRDVIYPYVYEKKDNTFRMAI
SIPNRIFPITALTLLALVYLPGVIAAILQLYRGTKYRRFPDWLDHWMLCRKQLGLVALGF
AFLHVLYTLVIPIRYYVRWRLGNLTVTQAILKKENPFSTSSAWLSDSYVALGILGFFLFV
LLGITSLPSVSNAVNWREFRFVQSKLGYLTLILCTAHTLVYGGKRFLSPSNLRWYLPAAY
VLGLIIPCTVLVIKFVLIMPCVDNTLTRIRQGWERNSKH
Target Bioclass
Enzyme
Family
STEAP family
Subcellular location
Cell membrane
Function
Integral membrane protein that functions as a NADPH-dependent ferric-chelate reductase, using NADPH from one side of the membrane to reduce a Fe(3+) chelate that is bound on the other side of the membrane. Mediates sequential transmembrane electron transfer from NADPH to FAD and onto heme, and finally to the Fe(3+) chelate. Can also reduce Cu(2+) to Cu(1+). Plays a role in systemic metabolic homeostasis, integrating inflammatory and metabolic responses. Associated with obesity and insulin-resistance. Involved in inflammatory arthritis, through the regulation of inflammatory cytokines. Inhibits anchorage-independent cell proliferation.
Uniprot ID
Q687X5
Ensemble ID
ENST00000301959.9
HGNC ID
HGNC:21923

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
COLO320 SNV: p.A186G .
COLO792 SNV: p.D29N .
HT115 SNV: p.R314Ter .
HT3 SNV: p.M13I DBIA    Probe Info 
IGR1 SNV: p.E334K; p.R413K .
K562 SNV: p.S457L .
KASUMI2 SNV: p.S49I .
LN18 SNV: p.T56N .
MCC13 SNV: p.R314Ter .
MEWO SNV: p.P114L .
TE1 SNV: p.R277L .
TOV21G Insertion: p.L194PfsTer37 .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C156(0.77); C5(2.84)  LDD3313  [1]
IA-alkyne
 Probe Info  		C156(0.00); C289(0.00)  LDD0162  [2]
TFBX
 Probe Info  		C5(0.00); C41(0.00)  LDD0148  [3]
PAL-AfBPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STS-1
 Probe Info  		N.A.  LDD0136  [4]
STS-2
 Probe Info  		N.A.  LDD0138  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0022  KB02 A2780 C23(1.17)  LDD2254  [1]
 LDCM0023  KB03 A2780 C156(2.01); C5(1.63)  LDD2671  [1]
 LDCM0024  KB05 Hs 936.T C156(0.77); C5(2.84)  LDD3313  [1]

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
3 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
4 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.