Details of the Target | Chem(Pro)²

General Information of Target

Target ID	LDTP07298
Target Name	Carboxypeptidase Z (CPZ)
Gene Name	CPZ
Gene ID	8532
Synonyms	Carboxypeptidase Z; CPZ; EC 3.4.17.-
3D Structure	Download 2D Sequence (FASTA) Download 3D Structure (PDB) Download
Sequence	MPPPLPLLLLTVLVVAAARPGCEFERNPAGECHRPPAADSATCVDLQLRTCSDAAYNHTT FPNLLQHRSWEVVEASSEYILLSVLHQLLEGQCNPDLRLLGCAVLAPRCEGGWVRRPCRH ICEGLREVCQPAFDAIDMAWPYFLDCHRYFTREDEGCYDPLEKLRGGLEADEALPSGLPP TFIRFSHHSYAQMVRVLRRTASRCAHVARTYSIGRSFDGRELLVIEFSSRPGQHELMEPE VKLIGNIHGNEVAGREMLIYLAQYLCSEYLLGNPRIQRLLNTTRIHLLPSMNPDGYEVAA AEGAGYNGWTSGRQNAQNLDLNRNFPDLTSEYYRLAETRGARSDHIPIPQHYWWGKVAPE TKAIMKWMQTIPFVLSASLHGGDLVVSYPFDFSKHPQEEKMFSPTPDEKMFKLLSRAYAD VHPMMMDRSENRCGGNFLKRGSIINGADWYSFTGGMSDFNYLHTNCFEITVELGCVKFPP EEALYILWQHNKESLLNFVETVHRGIKGVVTDKFGKPVKNARISVKGIRHDITTAPDGDY WRLLPPGIHIVIAQAPGYAKVIKKVIIPARMKRAGRVDFILQPLGMGPKNFIHGLRRTGP HDPLGGASSLGEATEPDPLRARRQPSADGSKPWWWSYFTSLSTHRPRWLLKY
Target Bioclass	Enzyme
Family	Peptidase M14 family
Subcellular location	Secreted, extracellular space, extracellular matrix
Function	Cleaves substrates with C-terminal arginine residues. Probably modulates the Wnt signaling pathway, by cleaving some undefined protein. May play a role in cleavage during prohormone processing.
Uniprot ID	Q66K79
Ensemble ID	ENST00000315782.6
HGNC ID	HGNC:2333

Target Site Mutations in Different Cell Lines

Cell line	Mutation details			Probe for labeling this protein in this cell
DEL	SNV: p.D320N			.
HCT15	SNV: p.W140Ter			.
HT3	SNV: p.S403F			.
IGROV1	SNV: p.C32Y			.
IPC298	SNV: p.E360K			.
KYSE410	SNV: p.M571I			.
MCC13	Substitution: p.R576C			.
NCIH1155	SNV: p.G605R			.
NCIH661	SNV: p.L100Q			.
SBC5	SNV: p.K362E			.
SNU1	SNV: p.C32Y			.

Probe(s) Labeling This Target

ABPP Probe

Click To Hide/Show 2 Probe Related to This Target

Probe name		Structure	Binding Site(Ratio)	Interaction ID	Ref
BTD Probe Info			C433(1.31)	LDD2118	[1]
Acrolein Probe Info			H120(0.00); C122(0.00)	LDD0217	[2]

Competitor(s) Related to This Target

Competitor ID	Name	Cell line	Binding Site(Ratio)	Interaction ID	Ref
LDCM0525	Nucleophilic fragment 25b	MDA-MB-231	C433(1.31)	LDD2118	[1]

References

1	Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29. Mass spectrometry data entry: PXD039908 , PXD029761
2	ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.