Details of the Target

General Information of Target

Target ID LDTP07220
Target Name Protein-cysteine N-palmitoyltransferase HHAT (HHAT)
Gene Name HHAT
Gene ID 55733
Synonyms
MART2; SKI1; Protein-cysteine N-palmitoyltransferase HHAT; EC 2.3.1.-; Hedgehog acyltransferase; Melanoma antigen recognized by T-cells 2; MART-2; Skinny hedgehog protein 1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MLPRWELALYLLASLGFHFYSFYEVYKVSREHEEELDQEFELETDTLFGGLKKDATDFEW
SFWMEWGKQWLVWLLLGHMVVSQMATLLARKHRPWILMLYGMWACWCVLGTPGVAMVLLH
TTISFCVAQFRSQLLTWLCSLLLLSTLRLQGVEEVKRRWYKTENEYYLLQFTLTVRCLYY
TSFSLELCWQQLPAASTSYSFPWMLAYVFYYPVLHNGPILSFSEFIKQMQQQEHDSLKAS
LCVLALGLGRLLCWWWLAELMAHLMYMHAIYSSIPLLETVSCWTLGGLALAQVLFFYVKY
LVLFGVPALLMRLDGLTPPALPRCVSTMFSFTGMWRYFDVGLHNFLIRYVYIPVGGSQHG
LLGTLFSTAMTFAFVSYWHGGYDYLWCWAALNWLGVTVENGVRRLVETPCIQDSLARYFS
PQARRRFHAALASCSTSMLILSNLVFLGGNEVGKTYWNRIFIQGWPWVTLSVLGFLYCYS
HVGIAWAQTYATD
Target Type
Literature-reported
Target Bioclass
Enzyme
Family
Membrane-bound acyltransferase family, HHAT subfamily
Subcellular location
Endoplasmic reticulum membrane
Function
Palmitoyl acyltransferase that catalyzes N-terminal palmitoylation of SHH; which is required for SHH signaling. It also catalyzes N-terminal palmitoylation of DHH. Promotes the transfer of palmitoyl-CoA from the cytoplasmic to the luminal side of the endoplasmic reticulum membrane, where SHH palmitoylation occurs. It is an essential factor for proper embryonic development and testicular organogenesis.
TTD ID
T01725
Uniprot ID
Q5VTY9
DrugMap ID
TT1VNCG
Ensemble ID
ENST00000261458.8
HGNC ID
HGNC:18270
ChEMBL ID
CHEMBL4296243

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
22RV1 SNV: p.G217R .
HCT116 SNV: p.G360D .
HCT15 Deletion: p.L276SfsTer10 .
HUPT3 Insertion: p.V72GfsTer12 .
Ishikawa (Heraklio) 02 ER SNV: p.W70L .
K562 SNV: p.D413E .
KYSE410 SNV: p.V152M .
NALM6 SNV: p.D57N .
NOMO1 SNV: p.V472I .
SNGM SNV: p.A369T .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C410(0.94)  LDD1511  [1]
IPM
 Probe Info  		N.A.  LDD2156  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0281  AC21 HEK-293T C410(0.99)  LDD1520  [1]
 LDCM0289  AC29 HEK-293T C410(0.99)  LDD1528  [1]
 LDCM0298  AC37 HEK-293T C410(0.90)  LDD1537  [1]
 LDCM0307  AC45 HEK-293T C410(0.95)  LDD1546  [1]
 LDCM0312  AC5 HEK-293T C410(0.85)  LDD1551  [1]
 LDCM0316  AC53 HEK-293T C410(0.88)  LDD1555  [1]
 LDCM0325  AC61 HEK-293T C410(0.89)  LDD1564  [1]
 LDCM0248  AKOS034007472 HEK-293T C410(0.94)  LDD1511  [1]
 LDCM0409  CL21 HEK-293T C410(1.63)  LDD1613  [1]
 LDCM0422  CL33 HEK-293T C410(1.60)  LDD1626  [1]
 LDCM0435  CL45 HEK-293T C410(1.47)  LDD1639  [1]
 LDCM0448  CL57 HEK-293T C410(1.49)  LDD1651  [1]
 LDCM0461  CL69 HEK-293T C410(1.53)  LDD1664  [1]
 LDCM0475  CL81 HEK-293T C410(1.29)  LDD1678  [1]
 LDCM0484  CL9 HEK-293T C410(1.31)  LDD1687  [1]
 LDCM0488  CL93 HEK-293T C410(1.27)  LDD1691  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Syntenin-1 (SDCBP) . O00560

References

1 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
2 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019