Details of the Target

General Information of Target

Target ID LDTP07114
Target Name Inactive glycosyltransferase 25 family member 3 (CERCAM)
Gene Name CERCAM
Gene ID 51148
Synonyms
CEECAM1; GLT25D3; KIAA1502; Inactive glycosyltransferase 25 family member 3; Cerebral endothelial cell adhesion molecule
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MRAARAAPLLQLLLLLGPWLEAAGVAESPLPAVVLAILARNAEHSLPHYLGALERLDYPR
ARMALWCATDHNVDNTTEMLQEWLAAVGDDYAAVVWRPEGEPRFYPDEEGPKHWTKERHQ
FLMELKQEALTFARNWGADYILFADTDNILTNNQTLRLLMGQGLPVVAPMLDSQTYYSNF
WCGITPQGYYRRTAEYFPTKNRQRRGCFRVPMVHSTFLASLRAEGADQLAFYPPHPNYTW
PFDDIIVFAYACQAAGVSVHVCNEHRYGYMNVPVKSHQGLEDERVNFIHLILEALVDGPR
MQASAHVTRPSKRPSKIGFDEVFVISLARRPDRRERMLASLWEMEISGRVVDAVDGWMLN
SSAIRNLGVDLLPGYQDPYSGRTLTKGEVGCFLSHYSIWEEVVARGLARVLVFEDDVRFE
SNFRGRLERLMEDVEAEKLSWDLIYLGRKQVNPEKETAVEGLPGLVVAGYSYWTLAYALR
LAGARKLLASQPLRRMLPVDEFLPIMFDQHPNEQYKAHFWPRDLVAFSAQPLLAAPTHYA
GDAEWLSDTETSSPWDDDSGRLISWSGSQKTLRSPRLDLTGSSGHSLQPQPRDEL
Target Bioclass
Enzyme
Family
Glycosyltransferase 25 family
Subcellular location
Endoplasmic reticulum lumen
Function Probable cell adhesion protein involved in leukocyte transmigration across the blood-brain barrier. Does not express any beta-galactosyltransferase activity in vitro.
Uniprot ID
Q5T4B2
Ensemble ID
ENST00000372838.9
HGNC ID
HGNC:23723

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
COLO320 Insertion: p.L84_A85insGGGGG .
COLO678 Insertion: p.L84_A85insGGGGG .
Ishikawa (Heraklio) 02 ER SNV: p.Y140C .
JURKAT SNV: p.D147N; p.Q491Ter .
LNCaP clone FGC SNV: p.E293Ter .
MEWO Insertion: p.A85GfsTer43 .
NCIH2172 SNV: p.V499L .
NCIH716 Insertion: p.A85GfsTer10
SNV: p.N237H		 .
OVCAR5 Insertion: p.A85GfsTer43 .
SKNSH SNV: p.Q569K .
SW1116 Insertion: p.A85GfsTer10 .
TOV21G SNV: p.S553Y .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C182(1.36)  LDD3332  [1]
m-APA
 Probe Info  		N.A.  LDD2231  [2]
W1
 Probe Info  		D433(0.00); E432(0.00); E435(0.00)  LDD0236  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0022  KB02 42-MG-BA C182(1.79)  LDD2244  [1]
 LDCM0023  KB03 A204 C182(2.03)  LDD2669  [1]
 LDCM0024  KB05 MKN45 C182(1.36)  LDD3332  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 10 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cell division cycle protein 23 homolog (CDC23) APC8/CDC23 family Q9UJX2
Palmitoyltransferase ZDHHC15 (ZDHHC15) DHHC palmitoyltransferase family Q96MV8
Inactive glycosyltransferase 25 family member 3 (CERCAM) Glycosyltransferase 25 family Q5T4B2
Prolyl 4-hydroxylase subunit alpha-3 (P4HA3) P4HA family Q7Z4N8
1-acylglycerol-3-phosphate O-acyltransferase ABHD5 (ABHD5) Peptidase S33 family Q8WTS1
Terminal nucleotidyltransferase 5B (TENT5B) TENT family Q96A09
E3 ubiquitin-protein ligase FANCL (FANCL) . Q9NW38
Glutaredoxin-3 (GLRX3) . O76003
Peptidyl-prolyl cis-trans isomerase FKBP7 (FKBP7) . Q9Y680
Probable E3 ubiquitin-protein ligase makorin-3 (MKRN3) . Q13064
Transcription factor
Click To Hide/Show 7 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Homeobox protein Hox-A1 (HOXA1) Antp homeobox family P49639
Homeobox protein OTX1 (OTX1) Paired homeobox family P32242
Rhox homeobox family member 2 (RHOXF2) Paired-like homeobox family Q9BQY4
POU domain, class 4, transcription factor 2 (POU4F2) POU transcription factor family Q12837
Homeobox protein VENTX (VENTX) . O95231
Pogo transposable element with ZNF domain (POGZ) . Q7Z3K3
Spermatogenesis- and oogenesis-specific basic helix-loop-helix-containing protein 1 (SOHLH1) . Q5JUK2
Other
Click To Hide/Show 13 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cysteine-rich tail protein 1 (CYSRT1) CYSRT1 family A8MQ03
Keratin-associated protein 3-3 (KRTAP3-3) KRTAP type 3 family Q9BYR6
Keratin-associated protein 6-2 (KRTAP6-2) KRTAP type 6 family Q3LI66
Keratin-associated protein 8-1 (KRTAP8-1) KRTAP type 8 family Q8IUC2
Keratin-associated protein 9-3 (KRTAP9-3) KRTAP type 9 family Q9BYQ3
Keratin-associated protein 15-1 (KRTAP15-1) PMG family Q3LI76
Keratin-associated protein 26-1 (KRTAP26-1) PMG family Q6PEX3
Vesicle transport protein SFT2A (SFT2D1) SFT2 family Q8WV19
Fibronectin type III domain-containing protein 11 (FNDC11) . Q9BVV2
Gem-associated protein 4 (GEMIN4) . P57678
Kelch-like protein 38 (KLHL38) . Q2WGJ6
Melanoma-associated antigen D1 (MAGED1) . Q9Y5V3
Ubiquitin-like protein 5 (UBL5) . Q9BZL1

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
3 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.