General Information of Target

Target ID LDTP07048
Target Name Alkaline ceramidase 2 (ACER2)
Gene Name ACER2
Gene ID 340485
Synonyms
ASAH3L; Alkaline ceramidase 2; AlkCDase 2; Alkaline CDase 2; haCER2; EC 3.5.1.-; EC 3.5.1.23; Acylsphingosine deacylase 3-like; N-acylsphingosine amidohydrolase 3-like
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MGAPHWWDQLQAGSSEVDWCEDNYTIVPAIAEFYNTISNVLFFILPPICMCLFRQYATCF
NSGIYLIWTLLVVVGIGSVYFHATLSFLGQMLDELAVLWVLMCALAMWFPRRYLPKIFRN
DRGRFKVVVSVLSAVTTCLAFVKPAINNISLMTLGVPCTALLIAELKRCDNMRVFKLGLF
SGLWWTLALFCWISDRAFCELLSSFNFPYLHCMWHILICLAAYLGCVCFAYFDAASEIPE
QGPVIKFWPNEKWAFIGVPYVSLLCANKKSSVKIT
Target Bioclass
Enzyme
Family
Alkaline ceramidase family
Subcellular location
Golgi apparatus membrane
Function
Golgi ceramidase that catalyzes the hydrolysis of ceramides into sphingoid bases like sphingosine and free fatty acids at alkaline pH. Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation. Has a better catalytic efficiency towards unsaturated long-chain ceramides, including C18:1-, C20:1- and C24:1-ceramides. Saturated long-chain ceramides and unsaturated very long-chain ceramides are also good substrates, whereas saturated very long-chain ceramides and short-chain ceramides are poor substrates. Also hydrolyzes dihydroceramides to produce dihydrosphingosine. It is the ceramidase that controls the levels of circulating sphingosine-1-phosphate and dihydrosphingosine-1-phosphate in plasma through their production by hematopoietic cells. Regulates cell proliferation, autophagy and apoptosis by the production of sphingosine and sphingosine-1-phosphate. As part of a p53/TP53-dependent pathway, promotes for instance autophagy and apoptosis in response to DNA damage. Through the production of sphingosine, may also regulate the function of the Golgi complex and regulate the glycosylation of proteins.
Uniprot ID
Q5QJU3
Ensemble ID
ENST00000340967.3
HGNC ID
HGNC:23675
ChEMBL ID
CHEMBL2331067

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
DU145 SNV: p.L88F .
MCAS SNV: p.D93H .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info 
C265(0.91)  LDD1511  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0281  AC21 HEK-293T C265(1.22)  LDD1520  [1]
 LDCM0289  AC29 HEK-293T C265(1.15)  LDD1528  [1]
 LDCM0298  AC37 HEK-293T C265(0.76)  LDD1537  [1]
 LDCM0307  AC45 HEK-293T C265(1.13)  LDD1546  [1]
 LDCM0312  AC5 HEK-293T C265(0.99)  LDD1551  [1]
 LDCM0316  AC53 HEK-293T C265(1.03)  LDD1555  [1]
 LDCM0325  AC61 HEK-293T C265(0.97)  LDD1564  [1]
 LDCM0248  AKOS034007472 HEK-293T C265(0.91)  LDD1511  [1]
 LDCM0409  CL21 HEK-293T C265(1.17)  LDD1613  [1]
 LDCM0422  CL33 HEK-293T C265(1.03)  LDD1626  [1]
 LDCM0435  CL45 HEK-293T C265(1.20)  LDD1639  [1]
 LDCM0448  CL57 HEK-293T C265(1.15)  LDD1651  [1]
 LDCM0461  CL69 HEK-293T C265(0.78)  LDD1664  [1]
 LDCM0475  CL81 HEK-293T C265(1.05)  LDD1678  [1]
 LDCM0484  CL9 HEK-293T C265(0.95)  LDD1687  [1]
 LDCM0488  CL93 HEK-293T C265(0.70)  LDD1691  [1]

References

1 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402