Details of the Target

General Information of Target

Target ID LDTP06923
Target Name Plasma membrane ascorbate-dependent reductase CYBRD1 (CYBRD1)
Gene Name CYBRD1
Gene ID 79901
Synonyms
DCYTB; FRRS3; Plasma membrane ascorbate-dependent reductase CYBRD1; EC 7.2.1.3; Cytochrome b reductase 1; Duodenal cytochrome b; Ferric-chelate reductase 3
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAMEGYWRFLALLGSALLVGFLSVIFALVWVLHYREGLGWDGSALEFNWHPVLMVTGFVF
IQGIAIIVYRLPWTWKCSKLLMKSIHAGLNAVAAILAIISVVAVFENHNVNNIANMYSLH
SWVGLIAVICYLLQLLSGFSVFLLPWAPLSLRAFLMPIHVYSGIVIFGTVIATALMGLTE
KLIFSLRDPAYSTFPPEGVFVNTLGLLILVFGALIFWIVTRPQWKRPKEPNSTILHPNGG
TEQGARGSMPAYSGNNMDKSDSELNSEVAARKRNLALDEAGQRSTM
Target Bioclass
Enzyme
Subcellular location
Cell membrane
Function
Plasma membrane reductase that uses cytoplasmic ascorbate as an electron donor to reduce extracellular Fe(3+) into Fe(2+). Probably functions in dietary iron absorption at the brush border of duodenal enterocytes by producing Fe(2+), the divalent form of iron that can be transported into enterocytes. It is also able to reduce extracellular monodehydro-L-ascorbate and may be involved in extracellular ascorbate regeneration by erythrocytes in blood. May also act as a ferrireductase in airway epithelial cells (Probable). May also function as a cupric transmembrane reductase.
Uniprot ID
Q53TN4
Ensemble ID
ENST00000321348.9
HGNC ID
HGNC:20797

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
AGS SNV: p.E197D .
HCC15 SNV: p.L175I .
NCIH1299 SNV: p.L45V .
NCIH1793 SNV: p.F58C .
NCIH196 SNV: p.V110F .
REH SNV: p.A27P .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K228(2.24)  LDD0277  [1]

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270