Details of the Target

General Information of Target

Target ID LDTP06783
Target Name tRNA pseudouridine synthase Pus10 (PUS10)
Gene Name PUS10
Gene ID 150962
Synonyms
CCDC139; DOBI; tRNA pseudouridine synthase Pus10; Hup10; EC 5.4.99.25; Coiled-coil domain-containing protein 139; tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MFPLTEENKHVAQLLLNTGTCPRCIFRFCGVDFHAPYKLPYKELLNELQKFLETEKDELI
LEVMNPPPKKIRLQELEDSIDNLSQNGEGRISVSHVGSTASKNSNLNVCNVCLGILQEFC
EKDFIKKVCQKVEASGFEFTSLVFSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRDDIV
QLKEAYKWITHPLFSEELGVPIDGKSLFEVSVVFAHPETVEDCHFLAAICPDCFKPAKNK
QSVFTRMAVMKALNKIKEEDFLKQFPCPPNSPKAVCAVLEIECAHGAVFVAGRYNKYSRN
LPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVDVRTLGNGRPFAIEL
VNPHRVHFTSQEIKELQQKINNSSNKIQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWT
NKAIQKKDIEFLNDIKDLKIDQKTPLRVLHRRPLAVRARVIHFMETQYVDEHHFRLHLKT
QAGTYIKEFVHGDFGRTKPNIGSLMNVTADILELDVESVDVDWPPALDD
Target Bioclass
Enzyme
Family
Pseudouridine synthase Pus10 family
Subcellular location
Nucleus
Function
Protein with different functions depending on its subcellular location: involved in miRNA processing in the nucleus and acts as a tRNA pseudouridylate synthase in the cytoplasm. In the cytoplasm, acts as a pseudouridylate synthase by catalyzing synthesis of pseudouridine(54) and pseudouridine(55) from uracil-54 and uracil-55, respectively, in the psi GC loop of a subset of tRNAs. tRNA pseudouridylate synthase activity is enhanced by the presence of 1-methyladenosine at position 53-61 of tRNAs. Does not show tRNA pseudouridylate synthase activity in the nucleus. In the nucleus, promotes primary microRNAs (pri-miRNAs) processing independently of its RNA pseudouridylate synthase activity. Binds pri-miRNAs. Modulator of TRAIL/TNFSF10-induced cell death via activation of procaspase-8 and BID cleavage. Required for the progression of the apoptotic signal through intrinsic mitochondrial cell death.
Uniprot ID
Q3MIT2
Ensemble ID
ENST00000316752.11
HGNC ID
HGNC:26505

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IPM
 Probe Info  		C29(0.00); C21(0.00)  LDD0241  [1]
DBIA
 Probe Info  		C29(2.94)  LDD3310  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0367  CL1 HEK-293T C29(0.96)  LDD1571  [3]
 LDCM0370  CL101 HEK-293T C29(1.35)  LDD1574  [3]
 LDCM0371  CL102 HEK-293T C29(0.88)  LDD1575  [3]
 LDCM0374  CL105 HEK-293T C29(1.23)  LDD1578  [3]
 LDCM0375  CL106 HEK-293T C29(1.24)  LDD1579  [3]
 LDCM0378  CL109 HEK-293T C29(0.93)  LDD1582  [3]
 LDCM0380  CL110 HEK-293T C29(0.78)  LDD1584  [3]
 LDCM0383  CL113 HEK-293T C29(1.23)  LDD1587  [3]
 LDCM0384  CL114 HEK-293T C29(0.81)  LDD1588  [3]
 LDCM0387  CL117 HEK-293T C29(1.20)  LDD1591  [3]
 LDCM0388  CL118 HEK-293T C29(1.04)  LDD1592  [3]
 LDCM0392  CL121 HEK-293T C29(0.99)  LDD1596  [3]
 LDCM0393  CL122 HEK-293T C29(0.90)  LDD1597  [3]
 LDCM0396  CL125 HEK-293T C29(1.09)  LDD1600  [3]
 LDCM0397  CL126 HEK-293T C29(0.78)  LDD1601  [3]
 LDCM0400  CL13 HEK-293T C29(1.02)  LDD1604  [3]
 LDCM0401  CL14 HEK-293T C29(0.98)  LDD1605  [3]
 LDCM0407  CL2 HEK-293T C29(0.93)  LDD1611  [3]
 LDCM0413  CL25 HEK-293T C29(1.09)  LDD1617  [3]
 LDCM0414  CL26 HEK-293T C29(0.95)  LDD1618  [3]
 LDCM0426  CL37 HEK-293T C29(1.19)  LDD1630  [3]
 LDCM0439  CL49 HEK-293T C29(1.63)  LDD1643  [3]
 LDCM0441  CL50 HEK-293T C29(0.93)  LDD1645  [3]
 LDCM0453  CL61 HEK-293T C29(1.37)  LDD1656  [3]
 LDCM0454  CL62 HEK-293T C29(1.01)  LDD1657  [3]
 LDCM0466  CL73 HEK-293T C29(1.13)  LDD1669  [3]
 LDCM0467  CL74 HEK-293T C29(1.16)  LDD1670  [3]
 LDCM0479  CL85 HEK-293T C29(1.17)  LDD1682  [3]
 LDCM0480  CL86 HEK-293T C29(0.77)  LDD1683  [3]
 LDCM0492  CL97 HEK-293T C29(1.33)  LDD1695  [3]
 LDCM0493  CL98 HEK-293T C29(0.91)  LDD1696  [3]
 LDCM0427  Fragment51 HEK-293T C29(0.82)  LDD1631  [3]
 LDCM0022  KB02 697 C29(2.02)  LDD2245  [2]
 LDCM0023  KB03 697 C29(3.22)  LDD2662  [2]
 LDCM0024  KB05 COLO792 C29(2.94)  LDD3310  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Lactoylglutathione lyase (GLO1) Glyoxalase I family Q04760
Ubiquitin carboxyl-terminal hydrolase 25 (USP25) Peptidase C19 family Q9UHP3
Other
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein Abitram (ABITRAM) ABITRAM family Q9NX38
Keratin, type I cytoskeletal 14 (KRT14) Intermediate filament family P02533
Keratin, type I cytoskeletal 16 (KRT16) Intermediate filament family P08779

References

1 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402