Details of the Target

General Information of Target

Target ID LDTP06729
Target Name Kelch-like protein 40 (KLHL40)
Gene Name KLHL40
Gene ID 131377
Synonyms
KBTBD5; SRYP; Kelch-like protein 40; Kelch repeat and BTB domain-containing protein 5; Sarcosynapsin
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MALGLEQAEEQRLYQQTLLQDGLKDMLDHGKFLDCVVRAGEREFPCHRLVLAACSPYFRA
RFLAEPERAGELHLEEVSPDVVAQVLHYLYTSEIALDEASVQDLFAAAHRFQIPSIFTIC
VSFLQKRLCLSNCLAVFRLGLLLDCARLAVAARDFICAHFTLVARDADFLGLSADELIAI
ISSDGLNVEKEEAVFEAVMRWAGSGDAEAQAERQRALPTVFESVRCRLLPRAFLESRVER
HPLVRAQPELLRKVQMVKDAHEGRITTLRKKKKGKDGAGAKEADKGTSKAKAEEDEEAER
ILPGILNDTLRFGMFLQDLIFMISEEGAVAYDPAANECYCASLSNQVPKNHVSLVTKENQ
VFVAGGLFYNEDNKEDPMSAYFLQFDHLDSEWLGMPPLPSPRCLFGLGEALNSIYVVGGR
EIKDGERCLDSVMCYDRLSFKWGESDPLPYVVYGHTVLSHMDLVYVIGGKGSDRKCLNKM
CVYDPKKFEWKELAPMQTARSLFGATVHDGRIIVAAGVTDTGLTSSAEVYSITDNKWAPF
EAFPQERSSLSLVSLVGTLYAIGGFATLETESGELVPTELNDIWRYNEEEKKWEGVLREI
AYAAGATFLPVRLNVLCLTKM
Target Bioclass
Other
Family
KLHL40 family
Subcellular location
Cytoplasm
Function
Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a key regulator of skeletal muscle development. The BCR(KLHL40) complex acts by mediating ubiquitination and degradation of TFDP1, thereby regulating the activity of the E2F:DP transcription factor complex. Promotes stabilization of LMOD3 by acting as a negative regulator of LMOD3 ubiquitination; the molecular process by which it negatively regulates ubiquitination of LMOD3 is however unclear.
Uniprot ID
Q2TBA0
Ensemble ID
ENST00000287777.5
HGNC ID
HGNC:30372

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HCC1806 SNV: p.R127G .
HCT15 SNV: p.L19M .
HEC1 SNV: p.Y90C .
HEC1B SNV: p.Y90C .
IGROV1 SNV: p.I180V .
JURKAT SNV: p.R147C .
MCC13 SNV: p.V82M .
NCIH358 SNV: p.R598L .
NOMO1 SNV: p.A566D .
OVK18 SNV: p.A197V .
PF382 SNV: p.A179T .
REC1 SNV: p.F117L .
REH SNV: p.R245H
Substitution: p.D175N		 .
RPMI7951 SNV: p.E282Ter .
UMUC3 SNV: p.A499S .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C403(1.70); C145(4.05)  LDD3401  [1]
Acrolein
 Probe Info  		N.A.  LDD0227  [2]
1d-yne
 Probe Info  		N.A.  LDD0357  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0022  KB02 RD C403(1.99); C145(3.12)  LDD2567  [1]
 LDCM0023  KB03 RD C403(1.84); C145(4.28)  LDD2984  [1]
 LDCM0024  KB05 RD C403(1.70); C145(4.05)  LDD3401  [1]
 LDCM0109  NEM HeLa N.A.  LDD0227  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Fibronectin type III and SPRY domain-containing protein 2 (FSD2) . A1L4K1
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Stabilizer of axonemal microtubules 1 (SAXO1) FAM154 family Q8IYX7
Protein TASOR 2 (TASOR2) TASOR family Q5VWN6

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
3 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.