Details of the Target

General Information of Target

Target ID LDTP06704
Target Name A-kinase anchor protein SPHKAP (SPHKAP)
Gene Name SPHKAP
Gene ID 80309
Synonyms
KIAA1678; SKIP; A-kinase anchor protein SPHKAP; SPHK1-interactor and AKAP domain-containing protein; Sphingosine kinase type 1-interacting protein
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MDGNSLLSVPSNLESSRMYDVLEPQQGRGCGSSGSGPGNSITACKKVLRSNSLLESTDYW
LQNQRMPCQIGFVEDKSENCASVCFVNLDVNKDECSTEHLQQKLVNVSPDLPKLISSMNV
QQPKENEIVVLSGLASGNLQADFEVSQCPWLPDICLVQCARGNRPNSTNCIIFEINKFLI
GLELVQERQLHLETNILKLEDDTNCSLSSIEEDFLTASEHLEEESEVDESRNDYENINVS
ANVLESKQLKGATQVEWNCNKEKWLYALEDKYINKYPTPLIKTERSPENLTKNTALQSLD
PSAKPSQWKREAVGNGRQATHYYHSEAFKGQMEKSQALYIPKDAYFSMMDKDVPSACAVA
EQRSNLNPGDHEDTRNALPPRQDGEVTTGKYATNLAESVLQDAFIRLSQSQSTLPQESAV
SVSVGSSLLPSCYSTKDTVVSRSWNELPKIVVVQSPDGSDAAPQPGISSWPEMEVSVETS
SILSGENSSRQPQSALEVALACAATVIGTISSPQATERLKMEQVVSNFPPGSSGALQTQA
PQGLKEPSINEYSFPSALCGMTQVASAVAVCGLGEREEVTCSVAPSGSLPPAAEASEAMP
PLCGLASMELGKEAIAKGLLKEAALVLTRPNTYSSIGDFLDSMNRRIMETASKSQTLCSE
NVVRNELAHTLSNVILRHSIDEVHHKNMIIDPNDNRHSSEILDTLMESTNQLLLDVICFT
FKKMSHIVRLGECPAVLSKETIRRRETEPSCQPSDPGASQAWTKATESSSSSPLSNSHNT
SLVINNLVDGMYSKQDKGGVRPGLFKNPTLQSQLSRSHRVPDSSTATTSSKEIYLKGIAG
EDTKSPHHSENECRASSEGQRSPTVSQSRSGSQEAEESIHPNTQEKYNCATSRINEVQVN
LSLLGDDLLLPAQSTLQTKHPDIYCITDFAEELADTVVSMATEIAAICLDNSSGKQPWFC
AWKRGSEFLMTPNVPCRSLKRKKESQGSGTAVRKHKPPRLSEIKRKTDEHPELKEKLMNR
VVDESMNLEDVPDSVNLFANEVAAKIMNLTEFSMVDGMWQAQGYPRNRLLSGDRWSRLKA
SSCESIPEEDSEARAYVNSLGLMSTLSQPVSRASSVSKQSSCESITDEFSRFMVNQMENE
GRGFELLLDYYAGKNASSILNSAMQQACRKSDHLSVRPSCPSKQSSTESITEEFYRYMLR
DIERDSRESASSRRSSQDWTAGLLSPSLRSPVCHRQSSMPDSRSPCSRLTVNVPIKANSL
DGFAQNCPQDFLSVQPVSSASSSGLCKSDSCLYRRGGTDHITNMLIHETWASSIEALMRK
NKIIVDDAEEADTEPVSGGSPSQAEKCANRLAASRMCSGPTLLVQESLDCPRKDSVTECK
QPPVSSLSKTASLTNHSPLDSKKETSSCQDPVPINHKRRSLCSREVPLIQIETDQREACA
GEPEPFLSKSSLLEEAEGHSNDKNIPDVVRGGDTAVSACQIHSDSLDTRDVPEAEASTEA
RAPDEAPNPPSSSEESTGSWTQLANEEDNPDDTSSFLQLSERSMSNGNSSATSSLGIMDL
DIYQESMPSSPMINELVEEKKILKGQSESTEAPASGPPTGTASPQRSLLVINFDLEPECP
DAELRATLQWIAASELGIPTIYFKKSQENRIEKFLDVVQLVHRKSWKVGDIFHAVVQYCK
MHEEQKDGRLSLFDWLLELG
Target Bioclass
Other
Family
AKAP110 family
Subcellular location
Cytoplasm
Function
Anchoring protein that binds preferentially to the type I regulatory subunit of c-AMP-dependent protein kinase (PKA type I) and targets it to distinct subcellular compartments. May act as a converging factor linking cAMP and sphingosine signaling pathways. Plays a regulatory role in the modulation of SPHK1.
Uniprot ID
Q2M3C7
Ensemble ID
ENST00000344657.5
HGNC ID
HGNC:30619

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A375 SNV: p.T741I .
A549 SNV: p.S1107I .
CCK81 SNV: p.A592T; p.R1131M .
COLO800 SNV: p.S1587L .
CORL88 SNV: p.E1192K .
GB2 SNV: p.E174Ter .
HCC44 SNV: p.P380S; p.G1297R .
HDQP1 SNV: p.A1352P .
HT115 SNV: p.P305S; p.L1636R .
IPC298 SNV: p.E1648K .
Ishikawa (Heraklio) 02 ER SNV: p.A765S .
JURKAT SNV: p.R310I .
KURAMOCHI SNV: p.P24Q .
KYSE510 SNV: p.R576T .
LS123 SNV: p.E613K .
MCC13 SNV: p.G251E; p.S484F .
MCC26 SNV: p.E876K; p.P1065L .
MELHO SNV: p.E372K .
MELJUSO SNV: p.G531E; p.S978F .
MEWO SNV: p.S1397N .
MFE319 Insertion: p.G637WfsTer30 .
NCIH2170 SNV: p.R745G .
NCIH2172 SNV: p.S302Ter .
NCIH3122 SNV: p.L814Ter .
NCIH358 SNV: p.C571S .
NCIH446 SNV: p.W470G .
OVK18 SNV: p.E943K .
PF382 SNV: p.N665D .
RKO SNV: p.P821S .
SHP77 Substitution: p.G1600Ter .
SKMEL24 SNV: p.R1068W .
SNU1196 SNV: p.Y391C .
SW1116 SNV: p.P301T; p.L589P .
SW403 SNV: p.R1470I .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
ONAyne
 Probe Info  		K617(6.25)  LDD0275  [1]
DBIA
 Probe Info  		C960(1.59)  LDD2270  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0022  KB02 BRX29 C960(1.59)  LDD2270  [2]
 LDCM0023  KB03 BRX29 C960(2.51)  LDD2687  [2]
 LDCM0024  KB05 BRX29 C960(2.36)  LDD3104  [2]

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840