Details of the Target

General Information of Target

Target ID LDTP06662
Target Name Rho GTPase-activating protein 44 (ARHGAP44)
Gene Name ARHGAP44
Gene ID 9912
Synonyms
KIAA0672; RICH2; Rho GTPase-activating protein 44; NPC-A-10; Rho-type GTPase-activating protein RICH2; RhoGAP interacting with CIP4 homologs protein 2; RICH-2
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MKKQFNRMRQLANQTVGRAEKTEVLSEDLLQVEKRLELVKQVSHSTHKKLTACLQGQQGA
EADKRSKKLPLTTLAQCLMEGSAILGDDTLLGKMLKLCGETEDKLAQELIHFELQVERDV
IEPLFLLAEVEIPNIQKQRKHLAKLVLDMDSSRTRWQQTSKSSGLSSSLQPAGAKADALR
EEMEEAANRVEICRDQLSADMYSFVAKEIDYANYFQTLIEVQAEYHRKSLTLLQAVLPQI
KAQQEAWVEKPSFGKPLEEHLTISGREIAFPIEACVTMLLECGMQEEGLFRVAPSASKLK
KLKAALDCCVVDVQEYSADPHAIAGALKSYLRELPEPLMTFELYDEWIQASNVQEQDKKL
QALWNACEKLPKANHNNIRYLIKFLSKLSEYQDVNKMTPSNMAIVLGPNLLWPQAEGNIT
EMMTTVSLQIVGIIEPIIQHADWFFPGEIEFNITGNYGSPVHVNHNANYSSMPSPDMDPA
DRRQPEQARRPLSVATDNMMLEFYKKDGLRKIQSMGVRVMDTNWVARRGSSAGRKVSCAP
PSMQPPAPPAELAAPLPSPLPEQPLDSPAAPALSPSGLGLQPGPERTSTTKSKELSPGSA
QKGSPGSSQGTACAGTQPGAQPGAQPGASPSPSQPPADQSPHTLRKVSKKLAPIPPKVPF
GQPGAMADQSAGQPSPVSLSPTPPSTPSPYGLSYPQGYSLASGQLSPAAAPPLASPSVFT
STLSKSRPTPKPRQRPTLPPPQPPTVNLSASSPQSTEAPMLDGMSPGESMSTDLVHFDIP
SIHIELGSTLRLSPLEHMRRHSVTDKRDSEEESESTAL
Target Bioclass
Other
Subcellular location
Cell projection, dendritic spine
Function
GTPase-activating protein (GAP) that stimulates the GTPase activity of Rho-type GTPases. Thereby, controls Rho-type GTPases cycling between their active GTP-bound and inactive GDP-bound states. Acts as a GAP at least for CDC42 and RAC1. In neurons, is involved in dendritic spine formation and synaptic plasticity in a specific RAC1-GAP activity. Limits the initiation of exploratory dendritic filopodia. Recruited to actin-patches that seed filopodia, binds specifically to plasma membrane sections that are deformed inward by acto-myosin mediated contractile forces. Acts through GAP activity on RAC1 to reduce actin polymerization necessary for filopodia formation. In association with SHANK3, promotes GRIA1 exocytosis from recycling endosomes and spine morphological changes associated to long-term potentiation.
Uniprot ID
Q17R89
Ensemble ID
ENST00000340825.7
HGNC ID
HGNC:29096

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CCK81 SNV: p.A569T .
CORL23 SNV: p.P413A .
DU145 SNV: p.C193Y .
HCT116 Deletion: p.G173VfsTer46 .
HCT15 SNV: p.S168I .
HEC1 SNV: p.A547T .
HEC1B SNV: p.A628T .
KYSE30 SNV: p.D312N .
LS180 SNV: p.N409T .
RL952 SNV: p.R490L .
SW756 SNV: p.S389Ter .
TOV21G Insertion: p.M760HfsTer19
SNV: p.G703V		 .
U937 SNV: p.E182Ter .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C367(1.14)  LDD1509  [1]
IA-alkyne
 Probe Info  		N.A.  LDD0165  [2]
NAIA_4
 Probe Info  		N.A.  LDD2226  [3]
AOyne
 Probe Info  		13.30  LDD0443  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0226  AC11 HEK-293T C367(1.14)  LDD1509  [1]
 LDCM0278  AC19 HEK-293T C367(1.08)  LDD1517  [1]
 LDCM0287  AC27 HEK-293T C367(1.17)  LDD1526  [1]
 LDCM0290  AC3 HEK-293T C367(1.13)  LDD1529  [1]
 LDCM0296  AC35 HEK-293T C367(1.13)  LDD1535  [1]
 LDCM0305  AC43 HEK-293T C367(1.16)  LDD1544  [1]
 LDCM0314  AC51 HEK-293T C367(1.09)  LDD1553  [1]
 LDCM0322  AC59 HEK-293T C367(1.20)  LDD1561  [1]
 LDCM0372  CL103 HEK-293T C367(1.18)  LDD1576  [1]
 LDCM0376  CL107 HEK-293T C367(1.19)  LDD1580  [1]
 LDCM0381  CL111 HEK-293T C367(0.90)  LDD1585  [1]
 LDCM0385  CL115 HEK-293T C367(1.21)  LDD1589  [1]
 LDCM0389  CL119 HEK-293T C367(1.28)  LDD1593  [1]
 LDCM0394  CL123 HEK-293T C367(1.11)  LDD1598  [1]
 LDCM0398  CL127 HEK-293T C367(0.94)  LDD1602  [1]
 LDCM0402  CL15 HEK-293T C367(1.59)  LDD1606  [1]
 LDCM0406  CL19 HEK-293T C367(1.09)  LDD1610  [1]
 LDCM0415  CL27 HEK-293T C367(1.17)  LDD1619  [1]
 LDCM0418  CL3 HEK-293T C367(1.03)  LDD1622  [1]
 LDCM0420  CL31 HEK-293T C367(1.10)  LDD1624  [1]
 LDCM0428  CL39 HEK-293T C367(1.20)  LDD1632  [1]
 LDCM0433  CL43 HEK-293T C367(1.18)  LDD1637  [1]
 LDCM0446  CL55 HEK-293T C367(1.21)  LDD1649  [1]
 LDCM0455  CL63 HEK-293T C367(1.16)  LDD1658  [1]
 LDCM0459  CL67 HEK-293T C367(1.24)  LDD1662  [1]
 LDCM0462  CL7 HEK-293T C367(1.10)  LDD1665  [1]
 LDCM0472  CL79 HEK-293T C367(1.23)  LDD1675  [1]
 LDCM0481  CL87 HEK-293T C367(0.98)  LDD1684  [1]
 LDCM0486  CL91 HEK-293T C367(1.22)  LDD1689  [1]
 LDCM0494  CL99 HEK-293T C367(0.97)  LDD1697  [1]
 LDCM0495  E2913 HEK-293T C367(1.10)  LDD1698  [1]
 LDCM0468  Fragment33 HEK-293T C367(1.20)  LDD1671  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cdc42-interacting protein 4 (TRIP10) FNBP1 family Q15642

References

1 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
2 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
3 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
4 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.