Details of the Target

General Information of Target

Target ID LDTP06492
Target Name NEDD8 (NEDD8)
Gene Name NEDD8
Gene ID 4738
Synonyms
NEDD8; Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; NEDD-8; Ubiquitin-like protein Nedd8
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYK
ILGGSVLHLVLALRGGGGLRQ
Target Type
Clinical trial
Target Bioclass
Other
Family
Ubiquitin family
Subcellular location
Nucleus
Function
Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis via its conjugation to a limited number of cellular proteins, such as cullins or p53/TP53 . Attachment of NEDD8 to cullins is critical for the recruitment of E2 to the cullin-RING-based E3 ubiquitin-protein ligase complex, thus facilitating polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. Attachment of NEDD8 to p53/TP53 inhibits p53/TP53 transcriptional activity. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M.
TTD ID
T93995
Uniprot ID
Q15843
DrugMap ID
TTNDC4K
Ensemble ID
ENST00000250495.10
HGNC ID
HGNC:7732
ChEMBL ID
CHEMBL4295831

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 7 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
ONAyne
 Probe Info  		K48(9.13)  LDD0275  [1]
5E-2FA
 Probe Info  		N.A.  LDD2235  [2]
ATP probe
 Probe Info  		K6(0.00); K48(0.00)  LDD0199  [3]
m-APA
 Probe Info  		N.A.  LDD2231  [2]
ATP probe
 Probe Info  		K48(0.00); K54(0.00)  LDD0035  [4]
NHS
 Probe Info  		K54(0.00); K48(0.00)  LDD0010  [5]
STPyne
 Probe Info  		N.A.  LDD0009  [5]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 8 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cullin-1 (CUL1) Cullin family Q13616
DNA polymerase iota (POLI) DNA polymerase type-Y family Q9UNA4
Protein KHNYN (KHNYN) N4BP1 family O15037
Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) Peptidase C12 family P09936
Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform (PPP2R2A) Phosphatase 2A regulatory subunit B family P63151
Ubiquitin-conjugating enzyme E2 K (UBE2K) Ubiquitin-conjugating enzyme family P61086
NEDD8-conjugating enzyme Ubc12 (UBE2M) Ubiquitin-conjugating enzyme family P61081
Small ribosomal subunit protein uS3 (RPS3) Universal ribosomal protein uS3 family P23396
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein S100-B (S100B) S-100 family P04271
Other
Click To Hide/Show 10 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Small ribosomal subunit protein eS7 (RPS7) Eukaryotic ribosomal protein eS7 family P62081
UV excision repair protein RAD23 homolog A (RAD23A) RAD23 family P54725
Stonin-2 (STON2) Stoned B family Q8WXE9
Tubulin beta chain (TUBB) Tubulin family P07437
Large ribosomal subunit protein uL30 (RPL7) Universal ribosomal protein uL30 family P18124
Large ribosomal subunit protein uL5 (RPL11) Universal ribosomal protein uL5 family P62913
Small ribosomal subunit protein uS10 (RPS20) Universal ribosomal protein uS10 family P60866
Gelsolin (GSN) Villin/gelsolin family P06396
F-box/WD repeat-containing protein 1A (BTRC) . Q9Y297
UBX domain-containing protein 7 (UBXN7) . O94888

The Drug(s) Related To This Target

Phase 3
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Mln4924 Small molecular drug D0ZD2M

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
3 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
4 Comparison of Quantitative Mass Spectrometry Platforms for Monitoring Kinase ATP Probe Uptake in Lung Cancer. J Proteome Res. 2018 Jan 5;17(1):63-75. doi: 10.1021/acs.jproteome.7b00329. Epub 2017 Nov 22.
Mass spectrometry data entry: PXD006095 , PXD006096
5 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764