Details of the Target

General Information of Target

Target ID LDTP06454
Target Name Phosphatidylinositol 4,5-bisphosphate 5-phosphatase A (INPP5J)
Gene Name INPP5J
Gene ID 27124
Synonyms
PIB5PA; PIPP; Phosphatidylinositol 4,5-bisphosphate 5-phosphatase A; EC 3.1.3.36; Inositol polyphosphate 5-phosphatase J; Phosphatidylinositol 1,3,4,5-tetrakisphosphate 5-phosphatase; EC 3.1.3.56; Phosphatidylinositol 1,4,5-trisphosphate 5-phosphatase; EC 3.1.3.56
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MEGQSSRGSRRPGTRAGLGSLPMPQGVAQTGAPSKVDSSFQLPAKKNAALGPSEPRLALA
PVGPRAAMSASSEGPRLALASPRPILAPLCTPEGQKTATAHRSSSLAPTSVGQLVMSASA
GPKPPPATTGSVLAPTSLGLVMPASAGPRSPPVTLGPNLAPTSRDQKQEPPASVGPKPTL
AASGLSLALASEEQPPELPSTPSPVPSPVLSPTQEQALAPASTASGAASVGQTSARKRDA
PAPRPLPASEGHLQPPAQTSGPTGSPPCIQTSPDPRLSPSFRARPEALHSSPEDPVLPRP
PQTLPLDVGQGPSEPGTHSPGLLSPTFRPGAPSGQTVPPPLPKPPRSPSRSPSHSPNRSP
CVPPAPDMALPRLGTQSTGPGRCLSPNLQAQEAPAPVTTSSSTSTLSSSPWSAQPTWKSD
PGFRITVVTWNVGTAMPPDDVTSLLHLGGGDDSDGADMIAIGLQEVNSMLNKRLKDALFT
DQWSELFMDALGPFNFVLVSSVRMQGVILLLFAKYYHLPFLRDVQTDCTRTGLGGYWGNK
GGVSVRLAAFGHMLCFLNCHLPAHMDKAEQRKDNFQTILSLQQFQGPGAQGILDHDLVFW
FGDLNFRIESYDLHFVKFAIDSDQLHQLWEKDQLNMAKNTWPILKGFQEGPLNFAPTFKF
DVGTNKYDTSAKKRKPAWTDRILWKVKAPGGGPSPSGRKSHRLQVTQHSYRSHMEYTVSD
HKPVAAQFLLQFAFRDDMPLVRLEVADEWVRPEQAVVRYRMETVFARSSWDWIGLYRVGF
RHCKDYVAYVWAKHEDVDGNTYQVTFSEESLPKGHGDFILGYYSHNHSILIGITEPFQIS
LPSSELASSSTDSSGTSSEGEDDSTLELLAPKSRSPSPGKSKRHRSRSPGLARFPGLALR
PSSRERRGASRSPSPQSRRLSRVAPDRSSNGSSRGSSEEGPSGLPGPWAFPPAVPRSLGL
LPALRLETVDPGGGGSWGPDREALAPNSLSPSPQGHRGLEEGGLGP
Target Bioclass
Enzyme
Family
Inositol 1,4,5-trisphosphate 5-phosphatase type II family
Subcellular location
Cytoplasm
Function
Inositol 5-phosphatase, which converts inositol 1,4,5-trisphosphate to inositol 1,4-bisphosphate. Also converts phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol 4-phosphate and inositol 1,3,4,5-tetrakisphosphate to inositol 1,3,4-trisphosphate in vitro. May be involved in modulation of the function of inositol and phosphatidylinositol polyphosphate-binding proteins that are present at membranes ruffles.
Uniprot ID
Q15735
Ensemble ID
ENST00000331075.10
HGNC ID
HGNC:8956

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CAMA1 SNV: p.S119L .
DU145 SNV: p.R503M .
HCT15 SNV: p.K687N .
HEC1 SNV: p.P312H; p.G462W .
HEC1B SNV: p.P312H; p.K687R .
IPC298 SNV: p.E649K .
JURKAT SNV: p.A98T .
MCC13 SNV: p.P519L .
NCIH1155 SNV: p.G532S .
OCIAML5 SNV: p.N539K .
RKO Deletion: p.P126QfsTer16 .
SNU1 SNV: p.W537Ter .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K617(8.33)  LDD0277  [1]
DBIA
 Probe Info  		C528(0.91)  LDD1492  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0022  KB02 HEK-293T C528(0.91)  LDD1492  [2]
 LDCM0023  KB03 HEK-293T C528(1.06)  LDD1497  [2]
 LDCM0024  KB05 HEK-293T C528(1.10)  LDD1502  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cell division cycle protein 23 homolog (CDC23) APC8/CDC23 family Q9UJX2
E3 ubiquitin-protein ligase pellino homolog 1 (PELI1) Pellino family Q96FA3
Zinc finger protein RFP (TRIM27) TRIM/RBCC family P14373
Tripartite motif-containing protein 54 (TRIM54) . Q9BYV2
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Homeobox protein MOX-1 (MEOX1) . P50221
Other
Click To Hide/Show 10 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
cAMP-dependent protein kinase type I-beta regulatory subunit (PRKAR1B) CAMP-dependent kinase regulatory chain family P31321
Epidermal growth factor receptor kinase substrate 8 (EPS8) EPS8 family Q12929
RNA-binding protein FXR2 (FXR2) FMR1 family P51116
Golgin subfamily A member 2 (GOLGA2) GOLGA2 family Q08379
Proteasome activator complex subunit 3 (PSME3) PA28 family P61289
RAD50-interacting protein 1 (RINT1) RINT1 family Q6NUQ1
Centrosomal protein of 70 kDa (CEP70) . Q8NHQ1
EF-hand domain-containing family member C2 (EFHC2) . Q5JST6
Heat shock factor 2-binding protein (HSF2BP) . O75031
PRKCA-binding protein (PICK1) . Q9NRD5

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402