Details of the Target

General Information of Target

Target ID LDTP06123
Target Name Growth factor receptor-bound protein 7 (GRB7)
Gene Name GRB7
Gene ID 2886
Synonyms
Growth factor receptor-bound protein 7; B47; Epidermal growth factor receptor GRB-7; GRB7 adapter protein
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MELDLSPPHLSSSPEDLCPAPGTPPGTPRPPDTPLPEEVKRSQPLLIPTTGRKLREEERR
ATSLPSIPNPFPELCSPPSQSPILGGPSSARGLLPRDASRPHVVKVYSEDGACRSVEVAA
GATARHVCEMLVQRAHALSDETWGLVECHPHLALERGLEDHESVVEVQAAWPVGGDSRFV
FRKNFAKYELFKSSPHSLFPEKMVSSCLDAHTGISHEDLIQNFLNAGSFPEIQGFLQLRG
SGRKLWKRFFCFLRRSGLYYSTKGTSKDPRHLQYVADVNESNVYVVTQGRKLYGMPTDFG
FCVKPNKLRNGHKGLRIFCSEDEQSRTCWLAAFRLFKYGVQLYKNYQQAQSRHLHPSCLG
SPPLRSASDNTLVAMDFSGHAGRVIENPREALSVALEEAQAWRKKTNHRLSLPMPASGTS
LSAAIHRTQLWFHGRISREESQRLIGQQGLVDGLFLVRESQRNPQGFVLSLCHLQKVKHY
LILPSEEEGRLYFSMDDGQTRFTDLLQLVEFHQLNRGILPCLLRHCCTRVAL
Target Bioclass
Other
Family
GRB7/10/14 family
Subcellular location
Cytoplasm
Function
Adapter protein that interacts with the cytoplasmic domain of numerous receptor kinases and modulates down-stream signaling. Promotes activation of down-stream protein kinases, including STAT3, AKT1, MAPK1 and/or MAPK3. Promotes activation of HRAS. Plays a role in signal transduction in response to EGF. Plays a role in the regulation of cell proliferation and cell migration. Plays a role in the assembly and stability of RNA stress granules. Binds to the 5'UTR of target mRNA molecules and represses translation of target mRNA species, when not phosphorylated. Phosphorylation impairs RNA binding and promotes stress granule disassembly during recovery after cellular stress.
Uniprot ID
Q14451
Ensemble ID
ENST00000309156.9
HGNC ID
HGNC:4567
ChEMBL ID
CHEMBL1649051

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
COLO678 SNV: p.H512P DBIA    Probe Info 
GB1 SNV: p.S441R .
LNCaP clone FGC Insertion: p.N306KfsTer16 .
NALM6 SNV: p.P77H .
UACC257 SNV: p.E109D .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IPM
 Probe Info  		C521(0.00); C319(0.00); C113(0.00)  LDD0241  [1]
DBIA
 Probe Info  		C544(1.73)  LDD3310  [2]
NAIA_5
 Probe Info  		C75(2.38)  LDD2227  [3]
IA-alkyne
 Probe Info  		C319(0.00); C521(0.00)  LDD0162  [4]
WYneN
 Probe Info  		N.A.  LDD0021  [5]
W1
 Probe Info  		C113(0.00); C521(0.00)  LDD0236  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0632  CL-Sc Hep-G2 C75(2.38)  LDD2227  [3]
 LDCM0022  KB02 769-P C351(2.88)  LDD2246  [2]
 LDCM0023  KB03 769-P C351(3.02)  LDD2663  [2]
 LDCM0024  KB05 COLO792 C544(1.73)  LDD3310  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 11 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Ubiquitin carboxyl-terminal hydrolase 2 (USP2) Peptidase C19 family O75604
Mast/stem cell growth factor receptor Kit (KIT) Tyr protein kinase family P10721
Platelet-derived growth factor receptor beta (PDGFRB) Tyr protein kinase family P09619
Receptor tyrosine-protein kinase erbB-2 (ERBB2) Tyr protein kinase family P04626
Receptor tyrosine-protein kinase erbB-3 (ERBB3) Tyr protein kinase family P21860
Ephrin type-B receptor 1 (EPHB1) Tyr protein kinase family P54762
Focal adhesion kinase 1 (PTK2) Tyr protein kinase family Q05397
Rho-related GTP-binding protein Rho6 (RND1) Rho family Q92730
Protein-glutamine gamma-glutamyltransferase 5 (TGM5) Transglutaminase family O43548
E3 ubiquitin-protein ligase TRIM36 (TRIM36) TRIM/RBCC family Q9NQ86
Tripartite motif-containing protein 43 (TRIM43) TRIM/RBCC family Q96BQ3
Transcription factor
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
CREB3 regulatory factor (CREBRF) BZIP family Q8IUR6
Androgen receptor (AR) Nuclear hormone receptor family P10275
Oligodendrocyte transcription factor 1 (OLIG1) . Q8TAK6
Immunoglobulin
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Lymphocyte antigen 6 complex locus protein G6f (LY6G6F) . Q5SQ64
Other
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Growth factor receptor-bound protein 7 (GRB7) GRB7/10/14 family Q14451
HCLS1-associated protein X-1 (HAX1) HAX1 family O00165
BTB/POZ domain-containing protein KCTD6 (KCTD6) . Q8NC69
Lymphocyte transmembrane adapter 1 (LAX1) . Q8IWV1
PRKCA-binding protein (PICK1) . Q9NRD5
SH3 and cysteine-rich domain-containing protein 3 (STAC3) . Q96MF2

References

1 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
4 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
5 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764