Details of the Target

General Information of Target

Target ID LDTP05813
Target Name Steroidogenic factor 1 (NR5A1)
Gene Name NR5A1
Gene ID 2516
Synonyms
AD4BP; FTZF1; SF1; Steroidogenic factor 1; SF-1; STF-1; hSF-1; Adrenal 4-binding protein; Fushi tarazu factor homolog 1; Nuclear receptor subfamily 5 group A member 1; Steroid hormone receptor Ad4BP
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MDYSYDEDLDELCPVCGDKVSGYHYGLLTCESCKGFFKRTVQNNKHYTCTESQSCKIDKT
QRKRCPFCRFQKCLTVGMRLEAVRADRMRGGRNKFGPMYKRDRALKQQKKAQIRANGFKL
ETGPPMGVPPPPPPAPDYVLPPSLHGPEPKGLAAGPPAGPLGDFGAPALPMAVPGAHGPL
AGYLYPAFPGRAIKSEYPEPYASPPQPGLPYGYPEPFSGGPNVPELILQLLQLEPDEDQV
RARILGCLQEPTKSRPDQPAAFGLLCRMADQTFISIVDWARRCMVFKELEVADQMTLLQN
CWSELLVFDHIYRQVQHGKEGSILLVTGQEVELTTVATQAGSLLHSLVLRAQELVLQLLA
LQLDRQEFVCLKFIILFSLDLKFLNNHILVKDAQEKANAALLDYTLCHYPHCGDKFQQLL
LCLVEVRALSMQAKEYLYHKHLGNEMPRNNLLIEMLQAKQT
Target Bioclass
Transcription factor
Family
Nuclear hormone receptor family, NR5 subfamily
Subcellular location
Nucleus
Function
Transcriptional activator. Essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional activity. Binds phosphatidylcholine. Binds phospholipids with a phosphatidylinositol (PI) headgroup, in particular PI(3,4)P2 and PI(3,4,5)P3. Activated by the phosphorylation of NR5A1 by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.
Uniprot ID
Q13285
Ensemble ID
ENST00000373588.9
HGNC ID
HGNC:7983
ChEMBL ID
CHEMBL4666

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IPM
 Probe Info  		C247(0.00); C266(0.00)  LDD0241  [1]
DBIA
 Probe Info  		C404(1.16)  LDD3310  [2]
W1
 Probe Info  		N.A.  LDD0236  [1]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STS-2
 Probe Info  		N.A.  LDD0139  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0022  KB02 22RV1 C404(1.25)  LDD2243  [2]
 LDCM0023  KB03 22RV1 C404(1.36)  LDD2660  [2]
 LDCM0024  KB05 COLO792 C404(1.16)  LDD3310  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Acid ceramidase (ASAH1) Acid ceramidase family Q13510
Replication factor C subunit 5 (RFC5) Activator 1 small subunits family P40937
Protein N-terminal glutamine amidohydrolase (NTAQ1) NTAQ1 family Q96HA8
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Nuclear receptor subfamily 0 group B member 1 (NR0B1) Nuclear hormone receptor family P51843
Other
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cyclin-dependent kinases regulatory subunit 1 (CKS1B) CKS family P61024
Tektin-4 (TEKT4) Tektin family Q8WW24
Cation channel sperm-associated targeting subunit tau (C2CD6) . Q53TS8
Cytoplasmic protein NCK2 (NCK2) . O43639

The Drug(s) Related To This Target

Investigative
Click To Hide/Show 2 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
(2r)-3-{[{[(2s)-23-dihydroxypropyl]Oxy}(Hydroxy)Phosphoryl]Oxy}-2-[(9e)-hexadec-9-enoyloxy]Propyl (9e)-octadec-9-enoate . DB04683
Phosphatidyl Ethanol . DB04752

References

1 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.