Details of the Target

General Information of Target

Target ID LDTP05335
Target Name Ammonium transporter Rh type A (RHAG)
Gene Name RHAG
Gene ID 6005
Synonyms
RH50; Ammonium transporter Rh type A; Erythrocyte membrane glycoprotein Rh50; Erythrocyte plasma membrane 50 kDa glycoprotein; Rh50A; Rhesus blood group family type A glycoprotein; Rh family type A glycoprotein; Rh type A glycoprotein; Rhesus blood group-associated ammonia channel; Rhesus blood group-associated glycoprotein; CD antigen CD241
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MRFTFPLMAIVLEIAMIVLFGLFVEYETDQTVLEQLNITKPTDMGIFFELYPLFQDVHVM
IFVGFGFLMTFLKKYGFSSVGINLLVAALGLQWGTIVQGILQSQGQKFNIGIKNMINADF
SAATVLISFGAVLGKTSPTQMLIMTILEIVFFAHNEYLVSEIFKASDIGASMTIHAFGAY
FGLAVAGILYRSGLRKGHENEESAYYSDLFAMIGTLFLWMFWPSFNSAIAEPGDKQCRAI
VNTYFSLAACVLTAFAFSSLVEHRGKLNMVHIQNATLAGGVAVGTCADMAIHPFGSMIIG
SIAGMVSVLGYKFLTPLFTTKLRIHDTCGVHNLHGLPGVVGGLAGIVAVAMGASNTSMAM
QAAALGSSIGTAVVGGLMTGLILKLPLWGQPSDQNCYDDSVYWKVPKTR
Target Type
Literature-reported
Target Bioclass
Transporter and channel
Family
Ammonium transporter (TC 2.A.49) family, Rh subfamily
Subcellular location
Membrane
Function
Component of the ankyrin-1 complex, a multiprotein complex involved in the stability and shape of the erythrocyte membrane. Heterotrimer with RHCE (RHAG)2(RHCE), that transports ammonium and its related derivative methylammonium, in both neutral and ionic forms, across the erythrocyte membrane. The transport of NH4(+) is electrogenic and masks the NH3 transport. Also, may act as a CO2 channel. In vitro, leaks monovalent cations. Moreover in erythrocyte, regulates RHD membrane expression and is associated with rhesus blood group antigen expression.
TTD ID
T65239
Uniprot ID
Q02094
DrugMap ID
TTVHFE8
Ensemble ID
ENST00000371175.10
HGNC ID
HGNC:10006

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HCT15 SNV: p.E34K .
HT3 SNV: p.G99E .
IGR1 SNV: p.K135N .
LS123 SNV: p.Q35Ter .
NCIH196 SNV: p.G169V .
NCIH661 SNV: p.G21V .
NUGC3 SNV: p.P223S .
OAW28 SNV: p.G376S .
TGBC52TKB Deletion: p.S103TfsTer13 .
VCAP SNV: p.M269I .
YSCCC SNV: p.Q55Ter .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C396(1.17)  LDD2324  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0022  KB02 EoL-1 C396(1.17)  LDD2324  [1]
 LDCM0023  KB03 HEL C396(2.13)  LDD2768  [1]
 LDCM0024  KB05 HEL C396(2.17)  LDD3185  [1]

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840