Details of the Target

General Information of Target

Target ID LDTP05167
Target Name G-protein-signaling modulator 2 (GPSM2)
Gene Name GPSM2
Gene ID 29899
Synonyms
LGN; G-protein-signaling modulator 2; Mosaic protein LGN
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MEENLISMREDHSFHVRYRMEASCLELALEGERLCKSGDCRAGVSFFEAAVQVGTEDLKT
LSAIYSQLGNAYFYLHDYAKALEYHHHDLTLARTIGDQLGEAKASGNLGNTLKVLGNFDE
AIVCCQRHLDISRELNDKVGEARALYNLGNVYHAKGKSFGCPGPQDVGEFPEEVRDALQA
AVDFYEENLSLVTALGDRAAQGRAFGNLGNTHYLLGNFRDAVIAHEQRLLIAKEFGDKAA
ERRAYSNLGNAYIFLGEFETASEYYKKTLLLARQLKDRAVEAQSCYSLGNTYTLLQDYEK
AIDYHLKHLAIAQELNDRIGEGRACWSLGNAYTALGNHDQAMHFAEKHLEISREVGDKSG
ELTARLNLSDLQMVLGLSYSTNNSIMSENTEIDSSLNGVRPKLGRRHSMENMELMKLTPE
KVQNWNSEILAKQKPLIAKPSAKLLFVNRLKGKKYKTNSSTKVLQDASNSIDHRIPNSQR
KISADTIGDEGFFDLLSRFQSNRMDDQRCCLQEKNCHTASTTTSSTPPKMMLKTSSVPVV
SPNTDEFLDLLASSQSRRLDDQRASFSNLPGLRLTQNSQSVLSHLMTNDNKEADEDFFDI
LVKCQGSRLDDQRCAPPPATTKGPTVPDEDFFSLILRSQGKRMDEQRVLLQRDQNRDTDF
GLKDFLQNNALLEFKNSGKKSADH
Target Bioclass
Other
Family
GPSM family
Subcellular location
Cytoplasm
Function
Plays an important role in mitotic spindle pole organization via its interaction with NUMA1. Required for cortical dynein-dynactin complex recruitment during metaphase. Plays a role in metaphase spindle orientation. Also plays an important role in asymmetric cell divisions. Has guanine nucleotide dissociation inhibitor (GDI) activity towards G(i) alpha proteins, such as GNAI1 and GNAI3, and thereby regulates their activity.
Uniprot ID
P81274
Ensemble ID
ENST00000264126.9
HGNC ID
HGNC:29501

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C614(2.13)  LDD3375  [1]
HHS-465
 Probe Info  		Y146(5.49)  LDD2237  [2]
NAIA_4
 Probe Info  		C161(0.00); C614(0.00)  LDD2226  [3]
Acrolein
 Probe Info  		N.A.  LDD0217  [4]
AOyne
 Probe Info  		13.80  LDD0443  [5]
NAIA_5
 Probe Info  		C325(0.00); C24(0.00); C516(0.00)  LDD2223  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0632  CL-Sc Hep-G2 C516(0.57)  LDD2227  [3]
 LDCM0022  KB02 HEK-293T C124(1.10)  LDD1492  [6]
 LDCM0023  KB03 HEK-293T C124(0.86)  LDD1497  [6]
 LDCM0024  KB05 OE33 C614(2.13)  LDD3375  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Volume-regulated anion channel subunit LRRC8E (LRRC8E) LRRC8 family Q6NSJ5
Other
Click To Hide/Show 7 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Guanine nucleotide-binding protein G(i) subunit alpha-1 (GNAI1) G-alpha family P63096
Guanine nucleotide-binding protein G(i) subunit alpha-3 (GNAI3) G-alpha family P08754
GAS2-like protein 1 (GAS2L1) GAS2 family Q99501
G-protein-signaling modulator 2 (GPSM2) GPSM family P81274
Nuclear mitotic apparatus protein 1 (NUMA1) . Q14980
Protein inscuteable homolog (INSC) . Q1MX18
Whirlin (WHRN) . Q9P202

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
3 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
4 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
5 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
6 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402