Details of the Target

General Information of Target

Target ID LDTP04968
Target Name Ubiquitin-conjugating enzyme E2 H (UBE2H)
Gene Name UBE2H
Gene ID 7328
Synonyms
Ubiquitin-conjugating enzyme E2 H; EC 2.3.2.23; (E3-independent) E2 ubiquitin-conjugating enzyme H; EC 2.3.2.24; E2 ubiquitin-conjugating enzyme H; UbcH2; Ubiquitin carrier protein H; Ubiquitin-conjugating enzyme E2-20K; Ubiquitin-protein ligase H
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSSPSPGKRRMDTDVVKLIESKHEVTILGGLNEFVVKFYGPQGTPYEGGVWKVRVDLPDK
YPFKSPSIGFMNKIFHPNIDEASGTVCLDVINQTWTALYDLTNIFESFLPQLLAYPNPID
PLNGDAAAMYLHRPEEYKQKIKEYIQKYATEEALKEQEEGTGDSSSESSMSDFSEDEAQD
MEL
Target Bioclass
Enzyme
Family
Ubiquitin-conjugating enzyme family
Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. E2 ubiquitin conjugating enzyme that transfers ubiquitin to MAEA, a core component of the CTLH E3 ubiquitin-protein ligase complex. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Capable, in vitro, to ubiquitinate histone H2A.
Uniprot ID
P62256
Ensemble ID
ENST00000355621.8
HGNC ID
HGNC:12484
ChEMBL ID
CHEMBL4105951

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
LNCaP clone FGC SNV: p.S171T .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0402  [1]
STPyne
 Probe Info  		K60(2.55); K64(3.96)  LDD0277  [2]
5E-2FA
 Probe Info  		N.A.  LDD2235  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Huntingtin (HTT) Huntingtin family P42858
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2 (GNB2) WD repeat G protein beta family P62879
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Melanoma-associated antigen 2 (MAGEA2; MAGEA2B) . P43356
Melanoma-associated antigen C2 (MAGEC2) . Q9UBF1

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377